Abstract:
:Cytological examination of cells from bronchoalveolar lavage (BAL) is commonly used for the diagnosis of lung cancer. Proteins released from lung cancer cells into BAL may serve as biomarkers for cancer detection. In this study, N-glycoproteins in eight cases of BAL fluid, as well as eight lung adenocarcinoma tissues and eight tumor-matched normal lung tissues, were analyzed using the solid-phase extraction of N-glycoprotein (SPEG), iTRAQ labeling, and liquid chromatography tandem mass spectrometry (LC-MS/MS). Of 80 glycoproteins found in BAL specimens, 32 were identified in both cancer BAL and cancer tissues, with levels of 25 glycoproteins showing at least a 2-fold difference between cancer and benign BAL. Among them, eight glycoproteins showed greater than 2-fold elevations in cancer BAL, including Neutrophil elastase (NE), Integrin alpha-M, Cullin-4B, Napsin A, lysosome-associated membrane protein 2 (LAMP2), Cathepsin D, BPI fold-containing family B member 2, and Neutrophil gelatinase-associated lipocalin. The levels of Napsin A in cancer BAL were further verified in independently collected 39 BAL specimens using an ELISA assay. Our study demonstrates that potential protein biomarkers in BAL fluid can be detected and quantified.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Li QK,Shah P,Li Y,Aiyetan PO,Chen J,Yung R,Molena D,Gabrielson E,Askin F,Chan DW,Zhang Hdoi
10.1021/pr400274wsubject
Has Abstractpub_date
2013-08-02 00:00:00pages
3689-96issue
8eissn
1535-3893issn
1535-3907journal_volume
12pub_type
杂志文章abstract::We describe a dual-column interface for parallel chromatography to improve throughput during LC-MS experimentation. The system employs a high-voltage switch to operate two capillary column/nanospray emitters fixed at the MS orifice. Sequentially loading one column while operating the second nearly doubles the LC-MS du...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr400738a
更新日期:2013-12-06 00:00:00
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journal_title:Journal of proteome research
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abstract::Mass spectrometry (MS) has become an accessible tool for whole proteome quantitation with the ability to characterize protein expression across thousands of proteins within a single experiment. A subset of MS quantification methods (e.g., SILAC and label-free) monitor the relative intensity of intact peptides, where t...
journal_title:Journal of proteome research
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journal_title:Journal of proteome research
pub_type: 杂志文章,评审
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journal_title:Journal of proteome research
pub_type: 杂志文章
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更新日期:2021-01-25 00:00:00
abstract::Cannabinoid 2 receptor (CB2R), a Class-A G-protein coupled receptor (GPCR), is a promising drug target under a wide array of pathological conditions. Rational drug design has been hindered due to our poor understanding of the structural features involved in ligand binding. Binding of a high-affinity biarylpyrazole inv...
journal_title:Journal of proteome research
pub_type: 杂志文章
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journal_title:Journal of proteome research
pub_type: 杂志文章
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更新日期:2011-02-04 00:00:00
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journal_title:Journal of proteome research
pub_type: 杂志文章
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journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr060503p
更新日期:2007-05-01 00:00:00
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journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.0c00066
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journal_title:Journal of proteome research
pub_type: 杂志文章
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更新日期:2007-07-01 00:00:00
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journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.6b00738
更新日期:2017-02-03 00:00:00
abstract::This paper describes a metabonomic study of prenatal disorders using nuclear magnetic resonance (NMR) spectroscopy of amniotic fluid (AF) collected in the second trimester of pregnancy, to search for metabolite markers of fetal malformations, prediagnostic gestational diabetes (GD), preterm delivery (PTD), early ruptu...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr100815q
更新日期:2010-11-05 00:00:00
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journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr9002118
更新日期:2010-01-01 00:00:00
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journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.6b00106
更新日期:2016-07-01 00:00:00
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journal_title:Journal of proteome research
pub_type: 杂志文章
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更新日期:2018-08-03 00:00:00
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journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.6b00824
更新日期:2017-02-03 00:00:00
abstract::Cyanobacteria developed efficient carbon concentrating mechanisms which significantly improve the photosynthetic performance and survival of cells under limiting CO(2) conditions. Dynamic changes of the Synechocystis proteome to CO(2) limitation were investigated using shotgun LC-MS/MS approach with isobaric tag for r...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr100651w
更新日期:2010-11-05 00:00:00
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journal_title:Journal of proteome research
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更新日期:2017-05-05 00:00:00
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journal_title:Journal of proteome research
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更新日期:2012-08-03 00:00:00
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journal_title:Journal of proteome research
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journal_title:Journal of proteome research
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journal_title:Journal of proteome research
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更新日期:2019-04-05 00:00:00
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更新日期:2007-11-01 00:00:00
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journal_title:Journal of proteome research
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更新日期:2005-01-01 00:00:00
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journal_title:Journal of proteome research
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