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Functional proteome analysis of the banana plant (Musa spp.) using de novo sequence analysis of derivatized peptides.

Abstract：

:We report the use of chemical derivatization with MALDI-MS/MS analysis for de novo sequence analysis. Using three frequently used homology-based search algorithms, we were able to identify more than 40 proteins from banana, a nonmodel plant with unsequenced genome. Furthermore, this approach allowed the identification of different isoforms. We also observed that the identification score obtained varied according to the position of the peptide sequences in the query using the MS-Blast algorithm.

journal_name

J Proteome Res

journal_title

Journal of proteome research

authors

Samyn B,Sergeant K,Carpentier S,Debyser G,Panis B,Swennen R,Van Beeumen J

doi

10.1021/pr0601943

subject

Has Abstract

pub_date

2007-01-01 00:00:00

pages

70-80

issue

1

eissn

1535-3893

issn

1535-3907

journal_volume

6

pub_type

杂志文章

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