Abstract：
:Reported in this work are the development of mass spectrometric immunoassay (MSIA) devices and methods for the qualitative analysis of IGF-1 and -2, and the rigorous quantification of IGF-1 from human plasma. A method involving addition of SDS in moderate concentration to unfractionated plasma for disrupting IGF/IGFBP complexes was initially developed. The method is suitable for the direct extraction of the IGFs and subsequent mass spectrometric analysis. Rat plasma, containing IGF-1 that is mass shifted from human IGF-1, was used as an internal reference standard (IRS) for the quantification of IGF-1 directly from human plasma. A standard curve with linear dynamic range of at least 2 orders of magnitude was constructed from serially diluted IGF-1 standards containing equal amounts of rat plasma. Using the standard curve, IGF-1 levels in plasma samples from eight individuals were determined. The limit of detection for the IGF-1 MSIA was also evaluated and established to be approximately 15 pM. The assay is rapid and can be performed in parallel via high-throughput robotics processing. Furthermore, the mass spectrometry aspect of the developed IGF-1 immunoassay offers a new dimension in the ongoing study of IGF-1 and related diseases.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Nelson RW,Nedelkov D,Tubbs KA,Kiernan UAdoi
10.1021/pr0499388keywords:
subject
Has Abstractpub_date
2004-07-01 00:00:00pages
851-5issue
4eissn
1535-3893issn
1535-3907journal_volume
3pub_type
杂志文章abstract：:Protein tyrosine nitration by oxidative and nitrate stress is important in the pathogenesis of many inflammatory or aging-related diseases. Mass spectrometry analysis of protein nitrotyrosine is very challenging because the non-nitrated peptides suppress the signals of the low-abundance nitrotyrosine (NT) peptides. No...
journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2017-08-04 00:00:00
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr901094j
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr8009929
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journal_title：Journal of proteome research
pub_type： 杂志文章
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journal_title：Journal of proteome research
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doi：10.1021/pr800792z
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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更新日期：2013-09-06 00:00:00
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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更新日期：2010-06-04 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr060514i
更新日期：2007-02-01 00:00:00
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journal_title：Journal of proteome research
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doi：10.1021/pr3004387
更新日期：2012-12-07 00:00:00
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journal_title：Journal of proteome research
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更新日期：2009-03-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.7b00703
更新日期：2018-03-02 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.6b00617
更新日期：2017-01-06 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr300691k
更新日期：2012-11-02 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr070239f
更新日期：2007-10-01 00:00:00
abstract：:Sperm proteomes have emerged for several species; however, the extent of species similarity is unknown. Sheep are an important agricultural species for which a comprehensive sperm proteome has not been produced. In addition, potential proteomic factors from seminal plasma that may contribute to improved fertility afte...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.6b00530
更新日期：2016-10-07 00:00:00
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journal_title：Journal of proteome research
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更新日期：2007-05-01 00:00:00
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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更新日期：2018-01-05 00:00:00
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journal_title：Journal of proteome research
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更新日期：2010-01-01 00:00:00
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journal_title：Journal of proteome research
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abstract：:Proteomics methods are being increasingly used to study archaeological and palaeontological bone, assisting in species identification and phylogenetic studies as well as improving our understanding of bone diagenesis. More recently, there are developing interests in the study of post-translational modifications, some ...
journal_title：Journal of proteome research
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更新日期：2017-02-03 00:00:00
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journal_title：Journal of proteome research
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更新日期：2008-04-01 00:00:00
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journal_title：Journal of proteome research
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更新日期：2013-01-04 00:00:00
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journal_title：Journal of proteome research
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