Urinary proteome profiling using microfluidic technology on a chip.

abstract：:T lymphocytes mediate cellular and humoral defense against foreign bodies or autoantigens. An understanding of T-cell information processing furthers studies of the immunological response. We describe a large-scale phosphorylation analysis of primary T cells using a multidimensional separation strategy, involving prep...

journal_title：Journal of proteome research

authors： Carrascal M,Ovelleiro D,Casas V,Gay M,Abian J

更新日期：2008-12-01 00:00:00

abstract：:The Baculoviral IAP repeat-containing protein 5 (BIRC5), also known as inhibitor of apoptosis protein survivin, is a member of the chromosomal passenger complex and a key player in mitosis. To investigate the function of BIRC5 in liver regeneration, we analyzed a hepatocyte-specific BIRC5-knockout mouse model using a ...

journal_title：Journal of proteome research

authors： Bracht T,Hagemann S,Loscha M,Megger DA,Padden J,Eisenacher M,Kuhlmann K,Meyer HE,Baba HA,Sitek B

更新日期：2014-06-06 00:00:00

abstract：:Although the legume-rhizobium symbiosis is a most-important biological process, there is a limited knowledge about the protein interaction network between host and symbiont. Using interolog- and domain-based approaches, we constructed an interspecies protein interactome containing 5115 protein-protein interactions bet...

journal_title：Journal of proteome research

authors： Zhang L,Liu JY,Gu H,Du Y,Zuo JF,Zhang Z,Zhang M,Li P,Dunwell JM,Cao Y,Zhang Z,Zhang YM

更新日期：2018-09-07 00:00:00

abstract：:Hematopoietic stem cell transplantation is the oldest and most successful form of stem cell therapy. High dose therapy (HDT) followed by hematopoietic stem cell transplantation allows physicians to administer increased amounts of chemotherapy and/or radiation while minimizing negative side effects such as damage to bl...

journal_title：Journal of proteome research

authors： Cano KE,Li L,Bhatia S,Bhatia R,Forman SJ,Chen Y

更新日期：2011-06-03 00:00:00

abstract：:The silk gland of the silkworm Bombyx mori undergoes programmed cell death (PCD) during pupal metamorphosis. On the basis of their morphological changes and the occurrence of a DNA ladder, the tissue cells were categorized into three groups: intact, committed, and dying. To identify the proteins involved in this proce...

journal_title：Journal of proteome research

authors： Jia SH,Li MW,Zhou B,Liu WB,Zhang Y,Miao XX,Zeng R,Huang YP

更新日期：2007-08-01 00:00:00

abstract：:Birth weight is an important indicator of maternal and fetal health and a predictor of health in later life. However, the determinants of variance in birth weight are still poorly understood. We aimed to identify the biological pathways, which may be perturbed by environmental exposures, that are important in determin...

journal_title：Journal of proteome research

authors： Robinson O,Keski-Rahkonen P,Chatzi L,Kogevinas M,Nawrot T,Pizzi C,Plusquin M,Richiardi L,Robinot N,Sunyer J,Vermeulen R,Vrijheid M,Vineis P,Scalbert A,Chadeau-Hyam M

更新日期：2018-03-02 00:00:00

abstract：:Sardinian alcohol-preferring (sP) and Sardinian alcohol-non preferring (sNP) rats have been selectively bred for opposite alcohol preference and consumption. Aiming to verify possible differences at the proteomics level between sP and sNP rats, we investigated the salivary proteome by a a liquid chromatography-mass sp...

journal_title：Journal of proteome research

authors： Cabras T,D'Alessandro A,Serrao S,Isola R,Iavarone F,Vincenzoni F,Colombo G,Ekström J,Messana I,Castagnola M

更新日期：2018-01-05 00:00:00

abstract：:To identify proteins that are involved in the molecular mechanisms of oxygen-induced retinopathy (OIR), a well-established model of blinding ischemic retinopathy, we quantitatively analyzed the retinal proteome in a mouse model of OIR. OIR was induced by exposing C57BL/6 mice on postnatal day 7 (P7) to 75% hyperoxia f...

journal_title：Journal of proteome research

authors： Kim SJ,Jin J,Kim YJ,Kim Y,Yu HG

更新日期：2012-11-02 00:00:00

abstract：:12-O-Tetradecanoyl-phorbol-13-acetate (TPA) is a plant derivative with multiple function as tumor promoter, differentiation revulsant or leukemia therapy drug. The molecular mechanism of its function is perplexing. Many studies have focused on the mechanism of TPA stimulation in tumor promotion of mouse models or term...

journal_title：Journal of proteome research

authors： Jiang P,Gan M,Huang H,Shen X,Wang S,Yao K

更新日期：2005-03-01 00:00:00

abstract：:A glycomic approach is developed to identify oligosaccharide markers for ovarian cancer by rapidly profiling globally released oligosaccharides. Glycoproteins shed by cancer cells are found in the supernatant (or conditioned media) of cultured cells. In this approach, shed glycoproteins are profiled for their oligosac...

journal_title：Journal of proteome research

authors： An HJ,Miyamoto S,Lancaster KS,Kirmiz C,Li B,Lam KS,Leiserowitz GS,Lebrilla CB

更新日期：2006-07-01 00:00:00

abstract：:In-depth, reproducible coverage of complex proteomes is challenging because the complexity of tryptic digests subjected to LC-MS/MS analysis frequently exceeds mass spectrometer analytical capacity, which results in undersampling of data. In this study, we used cancer cell lysates to systematically compare the commonl...

journal_title：Journal of proteome research

authors： Wang H,Chang-Wong T,Tang HY,Speicher DW

更新日期：2010-02-05 00:00:00

abstract：:Flooding has severe negative effects on soybean growth. To explore the flooding-responsive mechanisms in early-stage soybean, a phosphoproteomic approach was used. Two-day-old soybean plants were treated without or with flooding for 3, 6, 12, and 24 h, and root tip proteins were then extracted and analyzed at each tim...

journal_title：Journal of proteome research

authors： Yin X,Sakata K,Komatsu S

更新日期：2014-12-05 00:00:00

abstract：:Anoxygenic phototrophic bacteria are metabolically versatile and survive under different growth modes using diverse organic compounds, yet their metabolic diversity is largely unexplored. In the present study, we employed stable-isotope-assisted metabolic profiling to unravel the l-phenylalanine catabolism in Rubriviv...

journal_title：Journal of proteome research

authors： Mekala LP,Mohammed M,Chintalapati S,Chintalapati VR

更新日期：2018-01-05 00:00:00

abstract：:The mechanisms whereby Mycobacterium tuberculosis (Mtb) rewires the host metabolism in vivo are surprisingly unexplored. Here, we used three high-resolution mass spectrometry platforms to track altered lung metabolic changes associated with Mtb infection of mice. The multiplatform data sets were merged using consensus...

journal_title：Journal of proteome research

authors： Fernández-García M,Rey-Stolle F,Boccard J,Reddy VP,García A,Cumming BM,Steyn AJC,Rudaz S,Barbas C

更新日期：2020-05-01 00:00:00

abstract：:In this work, we developed a general perturbation theory and machine learning method for data mining of proteomes to discover new B-cell epitopes useful for vaccine design. The method predicts the epitope activity εq(cqj) of one query peptide (q-peptide) under a set of experimental query conditions (cqj). The method u...

journal_title：Journal of proteome research

authors： Martínez-Arzate SG,Tenorio-Borroto E,Barbabosa Pliego A,Díaz-Albiter HM,Vázquez-Chagoyán JC,González-Díaz H

更新日期：2017-11-03 00:00:00

abstract：:Protein post-translational modification by ubiquitin-fold modifier 1, UFM1, regulates many biological processes such as response to endoplasmic reticulum stress and regulation of tumor progression. A recent study has indicated that the UFM1-binding and PCI domain-containing protein 1 (UFBP1) is required for the conjug...

journal_title：Journal of proteome research

authors： Zhu Y,Lei Q,Li D,Zhang Y,Jiang X,Hu Z,Xu G

更新日期：2018-04-06 00:00:00

abstract：:Rhizobia, the nitrogen-fixing bacterial symbionts of legumes, represent an agricultural application of primary relevance and a model of plant-microbe molecular dialogues. We recently described rhizobium proteome alterations induced by plant flavonoids using iTRAQ. Herein, we further extend that experimentation, provin...

journal_title：Journal of proteome research

authors： Arrigoni G,Tolin S,Moscatiello R,Masi A,Navazio L,Squartini A

更新日期：2013-11-01 00:00:00

abstract：:The impact of low-dose ionizing radiation (IR) on the human brain has recently attracted attention due to the increased use of IR for diagnostic purposes. The aim of this study was to investigate low-dose radiation response in the hippocampus. Female B6C3F1 mice were exposed to total body irradiation with 0 (control),...

journal_title：Journal of proteome research

authors： Hladik D,Dalke C,von Toerne C,Hauck SM,Azimzadeh O,Philipp J,Ung MC,Schlattl H,Rößler U,Graw J,Atkinson MJ,Tapio S

更新日期：2020-01-03 00:00:00

abstract：:Recently, we reported comparative analysis of glycoproteins which express cancer-specific N-glycans on various cancer cells and identified 24 glycoproteins having polylactosamine (polyLacNAc)-type N-glycans that are abundantly present in malignant cells [ Mitsui et al., J. Pharm. Biomed. Anal. 2012 , 70 , 718 - 726 ]....

journal_title：Journal of proteome research

authors： Kinoshita M,Mitsui Y,Kakoi N,Yamada K,Hayakawa T,Kakehi K

更新日期：2014-02-07 00:00:00

abstract：:Quantifying peptides based on unique peptide fragment ions avoids the issue of ratio distortion that is commonly observed for reporter ion-based quantification approaches. Herein, we present a collision-induced dissociation-cleavable, isobaric acetyl-isoleucine-proline-glycine (Ac-IPG) tag, which conserves the merits ...

journal_title：Journal of proteome research

authors： Tian X,de Vries MP,Permentier HP,Bischoff R

更新日期：2020-09-04 00:00:00

abstract：:A well-described animal model was used to understand the molecular mechanisms of carcinogenesis and metastasis of gastric cancer at the protein level. Gastric cancer was induced in 12 Wistar rats by oral administration of N-methyl-N'-Nitro-N-Nitrosoguanidine (MNNG). The protein expression patterns of normal gastric ti...

journal_title：Journal of proteome research

authors： Chen J,Kähne T,Röcken C,Götze T,Yu J,Sung JJ,Chen M,Hu P,Malfertheiner P,Ebert MP

更新日期：2004-09-01 00:00:00

abstract：:IQGAP1 is a scaffolding protein that binds to a diverse array of signaling and structural molecules that are often associated with cell polarization and adhesion. Through interaction with its target proteins, IQGAP1 participates in multiple cellular functions, including Ca2+-calmodulin signaling, definition of cytoske...

journal_title：Journal of proteome research

authors： Meng X,Krokhin O,Cheng K,Ens W,Wilkins JA

更新日期：2007-02-01 00:00:00

abstract：:The two key steps for analyzing proteomic data generated by high-resolution MS are database searching and postprocessing. While the two steps are interrelated, studies on their combinatory effects and the optimization of these procedures have not been adequately conducted. Here, we investigated the performance of thre...

journal_title：Journal of proteome research

authors： Tu C,Sheng Q,Li J,Ma D,Shen X,Wang X,Shyr Y,Yi Z,Qu J

更新日期：2015-11-06 00:00:00

abstract：:Human saliva is a protein-rich, easily accessible source of potential local and systemic biomarkers to monitor changes that occur under pathological conditions; however, little is known about the changes in abundance associated with normal aging. In this study, we performed a comprehensive proteomic profiling of poole...

journal_title：Journal of proteome research

authors： Ambatipudi KS,Lu B,Hagen FK,Melvin JE,Yates JR

更新日期：2009-11-01 00:00:00

abstract：:Phosphorylation enhances IGFBP-1 binding to IGF-I, thereby limiting the bioavailability of IGF-I that may be important in fetal growth. Our goal in this study was to determine whether changes in site-specific IGFBP-1 phosphorylation were unique to fetal growth restriction. To establish a link, we compared IGFBP-1 phos...

journal_title：Journal of proteome research

authors： Abu Shehab M,Khosravi J,Han VK,Shilton BH,Gupta MB

更新日期：2010-04-05 00:00:00

abstract：:Ribosomal proteins of the model gram-positive bacterium B. subtilis 168 were extensively characterized in a proteomic study. Mass spectra of the 52 proteins expected to be constitutive components of the 70S ribosome were recorded. Peptide MS/MS analysis with an average sequence coverage of 85% supported the identifica...

journal_title：Journal of proteome research

authors： Lauber MA,Running WE,Reilly JP

更新日期：2009-09-01 00:00:00

abstract：:The processing of peptide tandem mass spectrometry data involves matching observed spectra against a sequence database. The ranking and calibration of these peptide-spectrum matches can be improved substantially using a machine learning postprocessor. Here, we describe our efforts to speed up one widely used postproce...

journal_title：Journal of proteome research

authors： Halloran JT,Zhang H,Kara K,Renggli C,The M,Zhang C,Rocke DM,Käll L,Noble WS

更新日期：2019-09-06 00:00:00

abstract：:Protein tyrosine nitration by oxidative and nitrate stress is important in the pathogenesis of many inflammatory or aging-related diseases. Mass spectrometry analysis of protein nitrotyrosine is very challenging because the non-nitrated peptides suppress the signals of the low-abundance nitrotyrosine (NT) peptides. No...

journal_title：Journal of proteome research

authors： Zhao Y,Zhang Y,Sun H,Maroto R,Brasier AR

更新日期：2017-08-04 00:00:00

abstract：:Src family kinases (SFKs) are the earliest known family of tyrosine kinases and are widely thought to play essential roles in cellular signal transduction. Although numerous functional analyses have been performed, no study has analyzed the specificity of all SFKs on an equal platform. To gain a better understanding o...

journal_title：Journal of proteome research

authors： Takeda H,Kawamura Y,Miura A,Mori M,Wakamatsu A,Yamamoto J,Isogai T,Matsumoto M,Nakayama KI,Natsume T,Nomura N,Goshima N

更新日期：2010-11-05 00:00:00

abstract：:The application of a data-independent acquisition (DIA) method ("SONAR") that employs a rapidly scanning quadrupole is described for the lipidomic analysis of complex biological extracts. Using this approach, the MS acquisition window can be varied between 1 and 25 Da, enabling the isolation of ions prior to their ent...

journal_title：Journal of proteome research

authors： King A,Baginski M,Morikawa Y,Rainville PD,Gethings LA,Wilson ID,Plumb RS

更新日期：2019-11-01 00:00:00