Proteome analysis of gastric cancer metastasis by two-dimensional gel electrophoresis and matrix assisted laser desorption/ionization-mass spectrometry for identification of metastasis-related proteins.

abstract：:Acute stress may trigger systemic biochemical and physiological changes in living organisms, leading to a rapid loss of homeostasis, which can be gradually reinstated by self-regulatory mechanisms and/or drug intervention strategy. However, such a sophisticated metabolic regulatory process has so far been poorly under...

journal_title：Journal of proteome research

authors： Wang X,Su M,Qiu Y,Ni Y,Zhao T,Zhou M,Zhao A,Yang S,Zhao L,Jia W

更新日期：2007-09-01 00:00:00

abstract：:Increased throughput as well as increased multiplexing of liquid chromatography coupled to selected reaction monitoring mass spectrometry (LC-SRM-MS) assays for protein quantification challenges routine data analysis. Despite the measurement of multiple transitions from multiple peptides, for clinical applications a s...

journal_title：Journal of proteome research

authors： van den Broek I,Mastali M,Mouapi K,Bystrom C,Bairey Merz CN,Van Eyk JE

更新日期：2020-06-05 00:00:00

abstract：:Neutrophilic granulocytes play a major role in the initiation and resolution of the inflammatory response, and demonstrate significant transcriptional and translational activity. Although much was known about neutrophils prior to the introduction of proteomics, the use of MS-based methodologies has provided an unprece...

journal_title：Journal of proteome research

authors： Tomazella GG,daSilva I,Thomé CH,Greene LJ,Koehler CJ,Thiede B,Wiker HG,de Souza GA

更新日期：2010-04-05 00:00:00

abstract：:Oxidation generates multiple diverse post-translational modifications resulting in changes in protein structure and function associated with a wide range of diseases. Of these modifications, carbonylations have often been used as hallmarks of oxidative damage. However, accumulating evidence supports the hypothesis tha...

journal_title：Journal of proteome research

authors： Rykær M,Svensson B,Davies MJ,Hägglund P

更新日期：2017-11-03 00:00:00

abstract：:Influenza viruses continue evolving and have the ability to cause a global pandemic, so it is very important to elucidate its pathogenesis and find new treatment methods. In recent years, proteomics has made important contributions to describing the dynamic interaction between influenza viruses and their hosts, especi...

journal_title：Journal of proteome research

authors： Zhang J,Peng Q,Zhao W,Sun W,Yang J,Liu N

更新日期：2021-01-01 00:00:00

abstract：:One of the ultimate goals of systems biology research is to obtain a comprehensive understanding of the control mechanisms of complex cellular metabolisms. Metabolic Flux Analysis (MFA) is a important method for the quantitative estimation of intracellular metabolic flows through metabolic pathways and the elucidation...

journal_title：Journal of proteome research

authors： Toya Y,Kono N,Arakawa K,Tomita M

更新日期：2011-08-05 00:00:00

abstract：:A proteome of a model organism, Caenorhabditis elegans, was analyzed by an integrated liquid chromatography (LC)-based protein identification system, which was constructed by microscale two-dimensional liquid chromatography (2DLC) coupled with electrospray ionization (ESI) tandem mass spectrometry (MS/MS) on a high-re...

journal_title：Journal of proteome research

authors： Mawuenyega KG,Kaji H,Yamuchi Y,Shinkawa T,Saito H,Taoka M,Takahashi N,Isobe T

更新日期：2003-01-01 00:00:00

abstract：:Pancreatic cystic neoplasms represent 10-15% of primary cystic masses of the pancreas. While pancreatic cysts are detected with an increasing frequency due to the use of advanced imaging modalities in clinical practice, the diagnosis of pancreatic cystic neoplasms remains unsatisfactory because available diagnostic te...

journal_title：Journal of proteome research

authors： Cuoghi A,Farina A,Z'graggen K,Dumonceau JM,Tomasi A,Hochstrasser DF,Genevay M,Lescuyer P,Frossard JL

更新日期：2011-05-06 00:00:00

abstract：:The process of extracting comprehensive proteome representations is a crucial step for many proteomic studies. While antibody microarrays are an evolving and promising methodology in proteomics, the issue of protein extraction from tissues for this kind of analysis has never been addressed. Here, we describe a single-...

journal_title：Journal of proteome research

authors： Alhamdani MS,Schröder C,Werner J,Giese N,Bauer A,Hoheisel JD

更新日期：2010-02-05 00:00:00

abstract：:Protein phosphorylation is a reversible post-translational modification that regulates many biological processes in almost all living forms. In the case of the hepatitis C virus (HCV), the nonstructural protein 5A (NS5A) is believed to transit between hypo- and hyper-phosphorylated forms that interact with host protei...

journal_title：Journal of proteome research

authors： Pan TC,Lo CW,Chong WM,Tsai CN,Lee KY,Chen PY,Liao JC,Yu MJ

更新日期：2019-07-05 00:00:00

abstract：:The term "spectral clustering" is sometimes used to refer to the clustering of mass spectrometry data. However, it also classically refers to a family of popular clustering algorithms. To avoid confusion, a more specific term could advantageously be coined. ...

journal_title：Journal of proteome research

authors： Borges H,Guibert R,Permiakova O,Burger T

更新日期：2019-01-04 00:00:00

abstract：:This nonrandomized phase I/II trial assessed the efficacy/tolerability of imatinib plus panitumumab in patients affected by metastatic colorectal cancer (mCRC) after stratification to treatment by selection of activated imatinib drug targets using reverse-phase protein array (RPPA). mCRC patients presenting with a bio...

journal_title：Journal of proteome research

authors： Pierobon M,Silvestri A,Spira A,Reeder A,Pin E,Banks S,Parasido E,Edmiston K,Liotta L,Petricoin E

更新日期：2014-06-06 00:00:00

abstract：:Proteomics by mass spectrometry (MS) allows the large-scale identification and quantitation of the cellular proteins in a given biological context. Systems biology studies from proteomics data are largely limited by the accuracy and coverage of quantitative proteomics along with missing values. Toward this end, statis...

journal_title：Journal of proteome research

authors： Aggarwal S,Talukdar NC,Yadav AK

更新日期：2019-06-07 00:00:00

abstract：:The protein expression pattern in the cytosol fraction of the adriamycin resistant MCF-7 cell line (MCF-7/ADR) was compared to that of the parental MCF-7 cell line using two-dimensional gel electrophoresis and mass spectrometry. Twenty proteins with altered abundances were identified and studied in MCF-7/ADR. Both up ...

journal_title：Journal of proteome research

authors： Gehrmann ML,Fenselau C,Hathout Y

更新日期：2004-05-01 00:00:00

abstract：:Stroke remains a major public health problem worldwide; it causes severe disability and is associated with high mortality rates. However, early diagnosis of stroke is difficult, and no reliable biomarkers are currently established. In this study, mass-spectrometry-based metabolomics was utilized to characterize the me...

journal_title：Journal of proteome research

authors： Liu P,Li R,Antonov AA,Wang L,Li W,Hua Y,Guo H,Wang L,Liu P,Chen L,Tian Y,Xu F,Zhang Z,Zhu Y,Huang Y

更新日期：2017-02-03 00:00:00

abstract：:Minimal hepatic encephalopathy (MHE) reduces quality of life of cirrhotic patients, predicts overt hepatic encephalopathy, and is associated with poor prognosis. We hypothesized that MHE arises once metabolic alterations derived from the liver reach a particular threshold. Our aim was to assess whether metabolic profi...

journal_title：Journal of proteome research

authors： Jiménez B,Montoliu C,MacIntyre DA,Serra MA,Wassel A,Jover M,Romero-Gomez M,Rodrigo JM,Pineda-Lucena A,Felipo V

更新日期：2010-10-01 00:00:00

abstract：:Schistosomes are blood-dwelling helminth parasites that cause schistosomiasis, a debilitating disease resulting in inflammation and, in extreme cases, multiple organ damage. Major challenges to control the transmission persist, and the discovery of protective antigens remains of critical importance for vaccine develop...

journal_title：Journal of proteome research

authors： Neves LX,Wilson RA,Brownridge P,Harman VM,Holman SW,Beynon RJ,Eyers CE,DeMarco R,Castro-Borges W

更新日期：2020-01-03 00:00:00

abstract：:In the past few years, the focus of phosphoproteomics has shifted from merely qualitative to quantitative and targeted studies. Tryptic digestion is a critical step that directly affects quantification and that can be impaired by phosphorylation. Therefore, we systematically characterized the digestion efficiency of 1...

journal_title：Journal of proteome research

authors： Dickhut C,Feldmann I,Lambert J,Zahedi RP

更新日期：2014-06-06 00:00:00

abstract：:Protein phosphorylation is a major mechanism of signal transduction in bacteria. Here, we analyzed the proteome and phosphoproteome of a wild-type strain of the food-borne pathogen Listeria monocytogenes that was grown in either chemically defined medium or rich medium containing glucose. We then compared these result...

journal_title：Journal of proteome research

authors： Misra SK,Moussan Désirée Aké F,Wu Z,Milohanic E,Cao TN,Cossart P,Deutscher J,Monnet V,Archambaud C,Henry C

更新日期：2014-12-05 00:00:00

abstract：:Although the "missing protein" is a temporary concept in C-HPP, the biological information for their "missing" could be an important clue in evolutionary studies. Here we classified missing-protein-encoding genes into two groups, the genes encoding PE2 proteins (with transcript evidence) and the genes encoding PE3/4 p...

journal_title：Journal of proteome research

authors： Xu A,Li G,Yang D,Wu S,Ouyang H,Xu P,He F

更新日期：2015-12-04 00:00:00

abstract：:Phencyclidine (PCP), a noncompetitive N-methyl-D-aspartate receptor antagonist, induces psychotomimetic effects in humans and animals. Administration of PCP to rodents is used as a preclinical model for schizophrenia; however, the molecular mechanisms underlying the symptoms remain largely unknown. Acute PCP treatment...

journal_title：Journal of proteome research

authors： Palmowski P,Rogowska-Wrzesinska A,Williamson J,Beck HC,Mikkelsen JD,Hansen HH,Jensen ON

更新日期：2014-03-07 00:00:00

abstract：:Protein microarrays consist of known proteins spotted onto solid substrates and are used to perform highly multivariate assessments of protein-binding interactions. Human protein arrays are routinely applied to pathogen detection, immune response biomarker profiling, and antibody specificity profiling. Here, we descri...

journal_title：Journal of proteome research

authors： Feng F,Ataca ST,Ran M,Wang Y,Breen M,Kepler TB

更新日期：2020-07-02 00:00:00

abstract：:Nostoc punctiforme ATCC 29133 is a photoautotrophic cyanobacterium with the capacity to fix atmospheric N 2. Its ability to mediate this process is similar to that described for Nostoc sp. PCC 7120, where vegetative cells differentiate into heterocysts. Quantitative proteomic investigations at both the filament level ...

journal_title：Journal of proteome research

authors： Ow SY,Noirel J,Cardona T,Taton A,Lindblad P,Stensjö K,Wright PC

更新日期：2009-01-01 00:00:00

abstract：:Patients with triple-negative breast cancer (TNBC) are unresponsive to endocrine and anti-HER2 pharmacotherapy, limiting their therapeutic options to chemotherapy. TNBC is frequently associated with abnormalities in the PI3K/AKT/mTOR signaling pathway; drugs targeting this pathway are currently being evaluated in thes...

journal_title：Journal of proteome research

authors： Euceda LR,Hill DK,Stokke E,Hatem R,El Botty R,Bièche I,Marangoni E,Bathen TF,Moestue SA

更新日期：2017-05-05 00:00:00

abstract：:Numerous reports have predicted/hypothesized a role for probenazole-induced protein (PBZ1) as a molecular marker in rice self-defense mechanism. However, the precise function of PBZ1 remains unknown. In the present study, we examined PBZ1 as a putative cell death marker in rice. For this, we focused our attention on a...

journal_title：Journal of proteome research

authors： Kim ST,Kim SG,Kang YH,Wang Y,Kim JY,Yi N,Kim JK,Rakwal R,Koh HJ,Kang KY

更新日期：2008-04-01 00:00:00

abstract：:Our aim was to analyze the plasma proteome in aspirin (acetylsalicylic acid [ASA])-sensitive and ASA-resistant coronary ischemic patients. Plasma from 19 ASA-sensitive and 19 ASA-resistant patients was analyzed. For the proteomic study, two-dimensional electrophoresis was performed. The expression of one isotype of th...

journal_title：Journal of proteome research

authors： López-Farré AJ,Mateos-Cáceres PJ,Sacristán D,Azcona L,Bernardo E,de Prada TP,Alonso-Orgaz S,Fernández-Arquero M,Fernández-Ortiz A,Macaya C

更新日期：2007-07-01 00:00:00

abstract：:The honeybees Apis mellifera ligustica (Aml) and Apis cerana cerana (Acc) are two different western and eastern bee species that evolved in distinct ecologies and developed specific antennal olfactory systems for their survival. Knowledge of how their antennal olfactory systems function in regards to the success of ea...

journal_title：Journal of proteome research

authors： Woltedji D,Song F,Zhang L,Gala A,Han B,Feng M,Fang Y,Li J

更新日期：2012-09-07 00:00:00

abstract：:Using an integrated transcriptomic and proteomic approach, we characterized the venom peptidome of the European red ant, Manica rubida. We identified 13 "myrmicitoxins" that share sequence similarities with previously identified ant venom peptides, one of them being identified as an EGF-like toxin likely resulting fro...

journal_title：Journal of proteome research

authors： Touchard A,Aili SR,Téné N,Barassé V,Klopp C,Dejean A,Kini RM,Mrinalini,Coquet L,Jouenne T,Lefranc B,Leprince J,Escoubas P,Nicholson GM,Treilhou M,Bonnafé E

更新日期：2020-04-03 00:00:00

abstract：:Sexual dimorphism is initialed by the components of the sex determination pathway and is most evident in gonads and germ cells. Although striking dimorphic expressions have been detected at the transcriptional level between the silkworm larval testis and the ovary, the sex-dimorphic expressions at the protein level ha...

journal_title：Journal of proteome research

authors： Chen JE,Li JY,You ZY,Liu LL,Liang JS,Ma YY,Chen M,Zhang HR,Jiang ZD,Zhong BX

更新日期：2013-06-07 00:00:00

abstract：:Frontotemporal lobar degeneration (FTLD) is an umbrella term for a heterogeneous group of young-onset dementias of uncertain prevalence and incidence worldwide. Atypical cases of FTLD with fused in sarcoma inclusions (aFTLD-U) have been described recently, but their molecular characterization is still due. Using shotg...

journal_title：Journal of proteome research

authors： Martins-de-Souza D,Guest PC,Mann DM,Roeber S,Rahmoune H,Bauder C,Kretzschmar H,Volk B,Baborie A,Bahn S

更新日期：2012-04-06 00:00:00