Abstract:
:The application of a data-independent acquisition (DIA) method ("SONAR") that employs a rapidly scanning quadrupole is described for the lipidomic analysis of complex biological extracts. Using this approach, the MS acquisition window can be varied between 1 and 25 Da, enabling the isolation of ions prior to their entering the collision cell. By rapidly scanning the resolving quadrupole window over a specified mass range, co-eluting precursor ions are transmitted sequentially into the collision cell, where collision energies are cycled between low and elevated levels to induce fragmentation. This method of data generation provides both precursor and fragment ion information at high specificity, allowing for greater accuracy of compound identification, whether using a database, spectral libraries, or comparison to authentic standards. The value of the approach in simplifying and "de-cluttering" the spectra of co-eluting lipids is shown with examples from lipidomic profiles obtained in investigations of the composition of organic extracts of livers obtained from SCID and chimeric liver-humanized mice administered under various experimental conditions.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
King A,Baginski M,Morikawa Y,Rainville PD,Gethings LA,Wilson ID,Plumb RSdoi
10.1021/acs.jproteome.9b00334subject
Has Abstractpub_date
2019-11-01 00:00:00pages
4055-4064issue
11eissn
1535-3893issn
1535-3907journal_volume
18pub_type
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