Abstract:
:New mass-tagging reagents for quantitative proteomics measurements have been designed using solid phase peptide synthesis technology. The solid phase mass tags have been used to accurately measure the relative amounts of cysteine-containing peptides in model peptide mixtures as well as in mixtures of tryptic digests in the femtomol range. Measurements were made using both matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS) and online reversed-phase capillary liquid chromatography coupled through a nanoelectrospray interface to an ion trap mass spectrometer (capillary LC/ESI-MS). Results of mass-tagging experiments obtained from these two mass spectrometry techniques and their relative advantages and disadvantages for identification and quantitation of mass tagged peptides are compared. These reagents provide a simple, rapid and cost-effective alternative to currently available mass tagging technologies.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Shi Y,Xiang R,Crawford JK,Colangelo CM,Horváth C,Wilkins JAdoi
10.1021/pr034081kkeywords:
subject
Has Abstractpub_date
2004-01-01 00:00:00pages
104-11issue
1eissn
1535-3893issn
1535-3907journal_volume
3pub_type
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