Abstract:
:Protein citrullination (or deimination), an irreversible post-translational modification, has been implicated in several physiological and pathological processes, including gene expression regulation, apoptosis, rheumatoid arthritis, and Alzheimer's disease. Several research studies have been carried out on citrullination under many conditions. However, until now, challenges in sample preparation and data analysis have made it difficult to confidently identify a citrullinated protein and assign the citrullinated site. To overcome these limitations, we generated a mouse hyper-citrullinated spectral library and set up coordinates to confidently identify and validate citrullinated sites. Using this workflow, we detect a four-fold increase in citrullinated proteome coverage across six mouse organs compared with the current state-of-the art techniques. Our data reveal that the subcellular distribution of citrullinated proteins is tissue-type-dependent and that citrullinated targets are involved in fundamental physiological processes, including the metabolic process. These data represent the first report of a hyper-citrullinated library for the mouse and serve as a central resource for exploring the role of citrullination in this organism.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Fert-Bober J,Venkatraman V,Hunter CL,Liu R,Crowgey EL,Pandey R,Holewinski RJ,Stotland A,Berman BP,Van Eyk JEdoi
10.1021/acs.jproteome.9b00118subject
Has Abstractpub_date
2019-05-03 00:00:00pages
2270-2278issue
5eissn
1535-3893issn
1535-3907journal_volume
18pub_type
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