Proteomic analysis of high-grade dysplastic cervical cells obtained from ThinPrep slides using laser capture microdissection and mass spectrometry.

abstract：:Large-scale phosphoproteomic analysis employing liquid chromatography-tandem mass spectrometry (LC-MS/MS) often requires a significant amount of manual manipulation of phosphopeptide datasets in the post-acquisition phase. To assist in this process, we have created software, PhosphoPIC (PhosphoPeptide Identification a...

journal_title：Journal of proteome research

authors： Hoffert JD,Wang G,Pisitkun T,Shen RF,Knepper MA

更新日期：2007-09-01 00:00:00

abstract：:Spontaneous preterm birth (SPTB) is a major contributor to perinatal morbidity and mortality. However, the diagnosis of preterm labor (PTL) that leads to preterm birth is difficult, and there is a pressing need for improved diagnosis. We utilized multidimensional liquid chromatography-tandem mass spectrometry (LC/LC-M...

journal_title：Journal of proteome research

authors： Pereira L,Reddy AP,Jacob T,Thomas A,Schneider KA,Dasari S,Lapidus JA,Lu X,Rodland M,Roberts CT Jr,Gravett MG,Nagalla SR

更新日期：2007-04-01 00:00:00

abstract：:The aim of current study was to investigate the metabolic changes associated with histidine supplementation in serum and urine metabolic signatures and serum amino acid (AA) profiles. Serum and urine 1H NMR-based metabolomics and serum AA profiles were employed in 32 and 37 obese women with metabolic syndrome (MetS) i...

journal_title：Journal of proteome research

authors： Du S,Sun S,Liu L,Zhang Q,Guo F,Li C,Feng R,Sun C

更新日期：2017-06-02 00:00:00

abstract：:Protein glycosylation was explored by direct nanoESI MS and MS/MS analysis of ZIC-HILIC-enriched proteolytic glycopeptides without further separation or purification. In a previous publication, we demonstrated that a direct MS-based analysis of proteolytic glycopeptides is feasible for a number of proteins (Henning , ...

journal_title：Journal of proteome research

authors： Neue K,Mormann M,Peter-Katalinić J,Pohlentz G

更新日期：2011-05-06 00:00:00

abstract：:We performed proteomic analyses of human olfactory epithelial tissue to identify missing proteins using liquid chromatography-tandem mass spectrometry. Using a next-generation proteomic pipeline with a < 1.0% false discovery rate at the peptide and protein levels, we identified 3731 proteins, among which five were mis...

journal_title：Journal of proteome research

authors： Hwang H,Jeong JE,Lee HK,Yun KN,An HJ,Lee B,Paik YK,Jeong TS,Yee GT,Kim JY,Yoo JS

更新日期：2018-12-07 00:00:00

abstract：:Post-translational nitration of proteins was analyzed by capillary reversed-phase high-performance liquid chromatography (RP-HPLC) on-line interfaced to electrospray ionization mass spectrometry (ESI--MS) or tandem mass spectrometry (ESI--MS/MS). Both methods were compared using a tryptic digest of bovine serum albumi...

journal_title：Journal of proteome research

authors： Walcher W,Franze T,Weller MG,Pöschl U,Huber CG

更新日期：2003-09-01 00:00:00

abstract：:A proteome of a model organism, Caenorhabditis elegans, was analyzed by an integrated liquid chromatography (LC)-based protein identification system, which was constructed by microscale two-dimensional liquid chromatography (2DLC) coupled with electrospray ionization (ESI) tandem mass spectrometry (MS/MS) on a high-re...

journal_title：Journal of proteome research

authors： Mawuenyega KG,Kaji H,Yamuchi Y,Shinkawa T,Saito H,Taoka M,Takahashi N,Isobe T

更新日期：2003-01-01 00:00:00

abstract：:Severe acute pancreatitis (SAP) is caused by complicated biological factors, and revealing its complex pathogenesis by single-target analysis is difficult. Systematic studies have developed slowly because extraction of degradable pancreatic proteins exposed to multiple proteases is challenging. We present integrated w...

journal_title：Journal of proteome research

authors： Wang C,Zhang Y,Tan J,Chen B,Sun L

更新日期：2020-06-05 00:00:00

abstract：:By foliar fortification with selenite, selenium (Se)-enriched rice with a higher Se content and grain yield has been generated. However, the regulatory mechanisms of Se response in rice grains remain unknown; therefore, we carried out a comparative proteomics study in Se-enriched rice grains by using two approaches in...

journal_title：Journal of proteome research

authors： Wang YD,Wang X,Ngai SM,Wong YS

更新日期：2013-02-01 00:00:00

abstract：:Histone linker proteins H1 and H5 were purified from chicken erythrocyte cell nuclei under nondenaturing conditions. The purified linker histones were analyzed using in-solution enzymatic digestions followed by nanoflow reverse-phase high-performance liquid chromatography tandem mass spectrometry. We have identified a...

journal_title：Journal of proteome research

authors： Snijders AP,Pongdam S,Lambert SJ,Wood CM,Baldwin JP,Dickman MJ

更新日期：2008-10-01 00:00:00

abstract：:A major challenge in the life sciences is the extraction of detailed molecular information from plants and animals that are not among the handful of exhaustively studied "model organisms." As a consequence, certain species with novel phenotypes are often ignored due to the lack of searchable databases, tractable genet...

journal_title：Journal of proteome research

authors： Russeth KP,Higgins L,Andrews MT

更新日期：2006-04-01 00:00:00

abstract：:Ozone (O3), a potent air pollutant and a significant greenhouse gas, has been recognized as a major component of global climate change. However, current increasing trends in its background level are projecting a more severe threat to natural and cultivated plants in the near future. The present study has been designed...

journal_title：Journal of proteome research

authors： Sarkar A,Rakwal R,Bhushan Agrawal S,Shibato J,Ogawa Y,Yoshida Y,Kumar Agrawal G,Agrawal M

更新日期：2010-09-03 00:00:00

abstract：:Ovarian cancer remains a deadly threat to women as the disease is often diagnosed in the late stages when the chance of survival is low. There are no good biomarkers available for early detection and only a few markers have shown clinical utility for prognosis, response to therapy and disease recurrence. We mined cond...

journal_title：Journal of proteome research

authors： Gunawardana CG,Kuk C,Smith CR,Batruch I,Soosaipillai A,Diamandis EP

更新日期：2009-10-01 00:00:00

abstract：:Quorum sensing (QS) in Xanthomonas oryzae pv. oryzicola (Xoc), the causal agent of bacterial leaf streak, is mediated by the diffusible signal factor (DSF). DSF-mediating QS has been shown to control virulence and a set of virulence-related functions; however, the expression profiles and functions of extracellular pro...

journal_title：Journal of proteome research

authors： Qian G,Zhou Y,Zhao Y,Song Z,Wang S,Fan J,Hu B,Venturi V,Liu F

更新日期：2013-07-05 00:00:00

abstract：:"Dry eye" is a multifactorial inflammatory disease affecting the ocular surface. Tear hyperosmolarity in dry eye contributes to inflammation and cell damage. Recent research efforts on dry eye have been directed toward biomarker discovery for diagnosis, response to treatment, and disease mechanisms. This study employe...

journal_title：Journal of proteome research

authors： Chen L,Li J,Guo T,Ghosh S,Koh SK,Tian D,Zhang L,Jia D,Beuerman RW,Aebersold R,Chan EC,Zhou L

更新日期：2015-09-04 00:00:00

abstract：:An unusual sulfotyrosine-, phosphoserine-containing motif was mapped on a differentially post-translationally modified 60 residue antimicrobial neuroendocrine peptide called chrombacin. The study was performed by high resolution FT MS using complementary fragmentation techniques. The peptide was analyzed at low levels...

journal_title：Journal of proteome research

authors： Taylor SW,Sun C,Hsieh A,Andon NL,Ghosh SS

更新日期：2008-02-01 00:00:00

abstract：:Wilson's Disease (WD), a copper transport disorder caused by a genetic defect in the ATP7B gene, has been a long time strong candidate for newborn screening (NBS), since early interventions can give better results by preventing irreversible neurological disability or liver cirrhosis. Several previous pilot studies mea...

journal_title：Journal of proteome research

authors： Jung S,Whiteaker JR,Zhao L,Yoo HW,Paulovich AG,Hahn SH

更新日期：2017-02-03 00:00:00

abstract：:Monoassociation of germfree Interleukin 10 gene deficient (IL-10-/-) 129SvEv but not wild-type mice with Enterococcus faecalis induces severe chronic colitis. Bacterial strain-specific effects on the development of chronic intestinal inflammation are not understood. We investigated the molecular mechanisms of E. faeca...

journal_title：Journal of proteome research

authors： Hoffmann M,Kim SC,Sartor RB,Haller D

更新日期：2009-03-01 00:00:00

abstract：:The majority of mitochondrial preproteins are targeted via N-terminal presequences that are cleaved upon import into the organelle. The essential mitochondrial processing protease (MPP) is assumed to cleave the majority of incoming precursors; however, only a small fraction of mitochondrial precursors have been experi...

journal_title：Journal of proteome research

authors： Burkhart JM,Taskin AA,Zahedi RP,Vögtle FN

更新日期：2015-11-06 00:00:00

abstract：:Many proteins can be modified by multiple types of post-translational modifications (Mtp-proteins). Although some post-translational modifications (PTMs) have recently been found to be associated with life-threatening diseases like cancers and neurodegenerative disorders, the underlying mechanisms remain enigmatic to ...

journal_title：Journal of proteome research

authors： Huang Q,Chang J,Cheung MK,Nong W,Li L,Lee MT,Kwan HS

更新日期：2014-06-06 00:00:00

abstract：:Metabolomic approaches have the potential to make an exceptional contribution to understanding how chemicals and other environmental stressors can affect both human and environmental health. However, the application of metabolomics to environmental exposures, although getting underway, has not yet been extensively exp...

journal_title：Journal of proteome research

authors： Miller MG

更新日期：2007-02-01 00:00:00

abstract：:N-Glycans are an important source of milk oligosaccharides. In addition to free oligosaccharides found in milk, N-glycans can also be utilized by gut microbes. A potential for milk N-glycans to act as gut microbe regulators in suckling animals has attracted considerable attention; however, sow milk N-glycans and their...

journal_title：Journal of proteome research

authors： Mu C,Cai Z,Bian G,Du Y,Ma S,Su Y,Liu L,Voglmeir J,Huang R,Zhu W

更新日期：2019-03-01 00:00:00

abstract：:Acute stress may trigger systemic biochemical and physiological changes in living organisms, leading to a rapid loss of homeostasis, which can be gradually reinstated by self-regulatory mechanisms and/or drug intervention strategy. However, such a sophisticated metabolic regulatory process has so far been poorly under...

journal_title：Journal of proteome research

authors： Wang X,Su M,Qiu Y,Ni Y,Zhao T,Zhou M,Zhao A,Yang S,Zhao L,Jia W

更新日期：2007-09-01 00:00:00

abstract：:We report a hybrid search method combining database and spectral library searches that allows for a straightforward approach to characterizing the error rates from the combined data. Using these methods, we demonstrate significantly increased sensitivity and specificity in matching peptides to tandem mass spectra. The...

journal_title：Journal of proteome research

authors： Cannon WR,Rawlins MM,Baxter DJ,Callister SJ,Lipton MS,Bryant DA

更新日期：2011-05-06 00:00:00

abstract：:Direct tissue profiling and imaging mass spectrometry (MS) provides a detailed assessment of the complex protein pattern within a tissue sample. MALDI MS analysis of thin tissue sections results in over of 500 individual protein signals in the mass range of 2 to 70 kDa that directly correlate with protein composition ...

journal_title：Journal of proteome research

authors： Chaurand P,Schwartz SA,Caprioli RM

更新日期：2004-03-01 00:00:00

abstract：:Candida albicans (C. albicans) is a worldwide cause of fungal infectious diseases. As a general post-translational modification (PTM), lysine acetylation of proteins play an important regulatory role in almost every cell. In our research, we used a high-resolution proteomic technique (LC-MS/MS) to present the comprehe...

journal_title：Journal of proteome research

authors： Zhou X,Qian G,Yi X,Li X,Liu W

更新日期：2016-08-05 00:00:00

abstract：:Coronary artery disease is one of the most frequent causes of morbidity and mortality worldwide. It is even more prevalent in patients with type 2 diabetes mellitus who suffer from obesity and increased accumulation of epicardial fat with a possible contributing role in the development of coronary artery disease. We p...

journal_title：Journal of proteome research

authors： Tomášová P,Čermáková M,Pelantová H,Vecka M,Kratochvílová H,Lipš M,Lindner J,Ivák P,Netuka I,Šedivá B,Haluzík M,Kuzma M

更新日期：2020-10-02 00:00:00

abstract：:Mass spectrometry is being used to identify protein biomarkers that can facilitate development of drug treatment. Mass spectrometry-based labeling proteomic experiments result in complex proteomic data that is hierarchical in nature often with small sample size studies. The generalized linear model (GLM) is the most p...

journal_title：Journal of proteome research

authors： D'Angelo G,Chaerkady R,Yu W,Hizal DB,Hess S,Zhao W,Lekstrom K,Guo X,White WI,Roskos L,Bowen MA,Yang H

更新日期：2017-09-01 00:00:00

abstract：:Major depressive disorder (MDD) is a socially detrimental psychiatric disorder, contributing to increased healthcare expenditures and suicide rates. However, no empirical laboratory-based tests are available to support the diagnosis of MDD. In this study, a NMR-based plasma metabonomic method for the diagnosis of MDD ...

journal_title：Journal of proteome research

authors： Zheng P,Gao HC,Li Q,Shao WH,Zhang ML,Cheng K,Yang DY,Fan SH,Chen L,Fang L,Xie P

更新日期：2012-03-02 00:00:00

abstract：:A detailed understanding of the relationships between the distinct metabolic compartments of blood and milk would be of potential benefit to our understanding of the physiology of lactation, and potentially for development of biomarkers for health and commercially relevant traits in dairy cattle. NMR methods were used...

journal_title：Journal of proteome research

authors： Maher AD,Hayes B,Cocks B,Marett L,Wales WJ,Rochfort SJ

更新日期：2013-03-01 00:00:00