当前位置： SCI文献检索 > JOURNAL OF PROTEOME RESEARCH期刊下所有文献 > Enterococcus faecalis strains differentially regulate Alix/AIP1 protein expression and ERK 1/2 activation in intestinal epithelial cells in the context of chronic experimental colitis.

Enterococcus faecalis strains differentially regulate Alix/AIP1 protein expression and ERK 1/2 activation in intestinal epithelial cells in the context of chronic experimental colitis.

Abstract：

:Monoassociation of germfree Interleukin 10 gene deficient (IL-10-/-) 129SvEv but not wild-type mice with Enterococcus faecalis induces severe chronic colitis. Bacterial strain-specific effects on the development of chronic intestinal inflammation are not understood. We investigated the molecular mechanisms of E. faecalis OG1RF (human clinical isolate, colitogenic) and E. faecalis ms2 (endogenous isolate from an IL-10-/- mouse) in initiating chronic experimental colitis using IL-10-/- mice. Monoassociation of IL-10-/- mice for 14 weeks revealed significant differences in colonic inflammation (3.6 +/- 0.2 and 2.4 +/- 0.6 for OG1RF and ms2, respectively) (n = 5 mice in each group) (histological scoring (0-4)). Consistent with the tissue pathology, gene expression of the pro-inflammatory chemokine interferon-gamma inducible protein-10 (IP-10) was significantly higher in intestinal epithelial cells (IEC) derived from E. faecalis OG1RF monoassociated IL-10-/- mice. We further compared the differentially E. faecalis induced colitis on the epithelial level by 2D-SDS PAGE coupled with MALDI-TOF MS. Proteome analysis identified 13 proteins which were differentially regulated during disease progression in the epithelium of E. faecalis-monoassociated IL-10-/- mice. Regulation of Alix/AIP1 protein expression and ERK1/2 phosphorylation was validated in primary IEC and epithelial cell lines, suggesting a protective role for Alix/AIP1 in the process of disease progression. Alix/AIP1 protein expression was further characterized in epithelial cell lines using siRNA-mediated knock-down. Our study demonstrates E. faecalis strain-specific induction of colitis in IL-10-/- mice after 14 weeks of monoassociation. Our study suggests that Alix/AIP1 protein expression and ERK1/2 activation are decreased in severe colitis.

journal_name

J Proteome Res

journal_title

Journal of proteome research

authors

Hoffmann M,Kim SC,Sartor RB,Haller D

doi

10.1021/pr800785m

subject

Has Abstract

pub_date

2009-03-01 00:00:00

pages

1183-92

issue

3

eissn

1535-3893

issn

1535-3907

journal_volume

8

pub_type

杂志文章

相关文献

JOURNAL OF PROTEOME RESEARCH文献大全
  • Proteomic Alterations in Response to Hypoxia Inducible Factor 2α in Normoxic Neuroblastoma Cells.

    abstract：:Hypoxia inducible factor (HIF)-2α protein expression in solid tumors promotes stem-like phenotype in cancer stem cells and increases tumorigenic potential in nonstem cancer cells. Recently, we have shown that HIF-1/2α gene expression is correlated to neuroblastoma (NB) poor survival and to undifferentiated tumor state...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/acs.jproteome.6b00457

    authors： Cimmino F,Pezone L,Avitabile M,Persano L,Vitale M,Sassi M,Bresolin S,Serafin V,Zambrano N,Scaloni A,Basso G,Iolascon A,Capasso M

    更新日期：2016-10-07 00:00:00

  • In-depth proteomic and glycomic analysis of the adult-stage Cooperia oncophora excretome/secretome.

    abstract：:Cooperia oncophora is one of the most common intestinal parasitic nematodes in cattle worldwide. To date, C. oncophora infections are treated using broad-spectrum anthelmintics. However, during the past decade, reports of anthelmintic resistance in this parasite species have emerged worldwide, necessitating new avenue...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr400114y

    authors： Borloo J,De Graef J,Peelaers I,Nguyen DL,Mitreva M,Devreese B,Hokke CH,Vercruysse J,Claerebout E,Geldhof P

    更新日期：2013-09-06 00:00:00

  • In vivo proximity labeling for the detection of protein-protein and protein-RNA interactions.

    abstract：:Accurate and sensitive detection of protein-protein and protein-RNA interactions is key to understanding their biological functions. Traditional methods to identify these interactions require cell lysis and biochemical manipulations that exclude cellular compartments that cannot be solubilized under mild conditions. H...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr500196b

    authors： Beck DB,Narendra V,Drury WJ 3rd,Casey R,Jansen PW,Yuan ZF,Garcia BA,Vermeulen M,Bonasio R

    更新日期：2014-12-05 00:00:00

  • Probity: a protein identification algorithm with accurate assignment of the statistical significance of the results.

    abstract：:An algorithm for protein identification based on mass spectrometric proteolytic peptide mapping and genome database searching is presented. The algorithm ranks database proteins based on direct calculation of the probability of random matching and assigns the statistical significance to each result. We investigate the...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr034048y

    authors： Eriksson J,Fenyö D

    更新日期：2004-01-01 00:00:00

  • IDAWG: Metabolic incorporation of stable isotope labels for quantitative glycomics of cultured cells.

    abstract：:Robust quantification is an essential component of comparative -omic strategies. In this regard, glycomics lags behind proteomics. Although various isotope-tagging and direct quantification methods have recently enhanced comparative glycan analysis, a cell culture labeling strategy, that could provide for glycomics th...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr8010028

    authors： Orlando R,Lim JM,Atwood JA 3rd,Angel PM,Fang M,Aoki K,Alvarez-Manilla G,Moremen KW,York WS,Tiemeyer M,Pierce M,Dalton S,Wells L

    更新日期：2009-08-01 00:00:00

  • Selective Affinity Enrichment of Nitrotyrosine-Containing Peptides for Quantitative Analysis in Complex Samples.

    abstract：:Protein tyrosine nitration by oxidative and nitrate stress is important in the pathogenesis of many inflammatory or aging-related diseases. Mass spectrometry analysis of protein nitrotyrosine is very challenging because the non-nitrated peptides suppress the signals of the low-abundance nitrotyrosine (NT) peptides. No...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/acs.jproteome.7b00275

    authors： Zhao Y,Zhang Y,Sun H,Maroto R,Brasier AR

    更新日期：2017-08-04 00:00:00

  • Selenite-induced toxicity in cancer cells is mediated by metabolic generation of endogenous selenium nanoparticles.

    abstract：:Selenite has been a touted cancer chemopreventative agent but generates conflicting outcomes. Multiple mechanisms of selenite cytotoxicity in cancer cells are thought to be induced by metabolites of selenite. We observed that intracellular metabolism of selenite generates endogenous selenium nanoparticles (SeNPs) in c...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr501086e

    authors： Bao P,Chen Z,Tai RZ,Shen HM,Martin FL,Zhu YG

    更新日期：2015-02-06 00:00:00

  • Proteomic analysis of Pinus radiata needles: 2-DE map and protein identification by LC/MS/MS and substitution-tolerant database searching.

    abstract：:Pinus radiata is one of the most economically important forest tree species, with a worldwide production of around 370 million m (3) of wood per year. Current selection of elite trees to be used in conservation and breeding programes requires the physiological and molecular characterization of available populations. T...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr7006285

    authors： Valledor L,Castillejo MA,Lenz C,Rodríguez R,Cañal MJ,Jorrín J

    更新日期：2008-07-01 00:00:00

  • Metabolomic analysis reveals a unique urinary pattern in normozoospermic infertile men.

    abstract：:Normozoospermic infertility has become a common and important health problem worldwide. We designed this metabolomic case-control study to investigate the possible mechanism and urinary biomarkers of normozoospermic infertility. Normozoospermic infertile cases (n = 71) and fertile controls (n = 47) were recruited. A u...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr5003142

    authors： Zhang J,Mu X,Xia Y,Martin FL,Hang W,Liu L,Tian M,Huang Q,Shen H

    更新日期：2014-06-06 00:00:00

  • Automated N-glycopeptide identification using a combination of single- and tandem-MS.

    abstract：:We describe Peptoonist, a program that can automatically identify the glycans (sugars) present at each N-glycosylation site of a protein. The input to Peptoonist is a series of mass spectra, both MS and MS/MS, obtained from a liquid chromatography (LC) run of proteolytically digested purified glycoproteins. The progra...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr070239f

    authors： Goldberg D,Bern M,Parry S,Sutton-Smith M,Panico M,Morris HR,Dell A

    更新日期：2007-10-01 00:00:00

  • Analysis of serum haptoglobin fucosylation in hepatocellular carcinoma and liver cirrhosis of different etiologies.

    abstract：:We have developed herein a quantitative mass spectrometry-based approach to analyze the etiology-related alterations in fucosylation degree of serum haptoglobin in patients with liver cirrhosis and hepatocellular carcinoma (HCC). The three most common etiologies, including infection with hepatitis B virus (HBV), infec...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr500128t

    authors： Zhu J,Lin Z,Wu J,Yin H,Dai J,Feng Z,Marrero J,Lubman DM

    更新日期：2014-06-06 00:00:00

  • AKT1 and AKT2 induce distinct phosphorylation patterns in HL-1 cardiac myocytes.

    abstract：:The protein kinase AKT is a central kinase in the heart and has a major impact on growth/hypertrophy, survival/apoptosis, and metabolism. To gain more insight into AKT isoform-specific signaling at the molecular level, we investigated the phosphoproteome of HL-1 cardiomyocytes carrying AKT1 or AKT2 isoform-specific kn...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr500131g

    authors： Reinartz M,Raupach A,Kaisers W,Gödecke A

    更新日期：2014-10-03 00:00:00

  • TagRecon: high-throughput mutation identification through sequence tagging.

    abstract：:Shotgun proteomics produces collections of tandem mass spectra that contain all the data needed to identify mutated peptides from clinical samples. Identifying these sequence variations, however, has not been feasible with conventional database search strategies, which require exact matches between observed and expect...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr900850m

    authors： Dasari S,Chambers MC,Slebos RJ,Zimmerman LJ,Ham AJ,Tabb DL

    更新日期：2010-04-05 00:00:00

  • Novel Mechanistic Insights into Bacterial Fluoroquinolone Resistance.

    abstract：:Advancements in studies on the evolutionary mechanisms underlying bacterial antibiotic resistance are unclear. This study aimed to investigate the evolutionary mechanism underlying bacterial antibiotic resistance using isobaric tags for relative and absolute quantitation-based quantitative proteomics along with functi...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/acs.jproteome.9b00410

    authors： Du GF,Zheng YD,Chen J,He QY,Sun X

    更新日期：2019-11-01 00:00:00

  • Impact of digestion conditions on phosphoproteomics.

    abstract：:In the past few years, the focus of phosphoproteomics has shifted from merely qualitative to quantitative and targeted studies. Tryptic digestion is a critical step that directly affects quantification and that can be impaired by phosphorylation. Therefore, we systematically characterized the digestion efficiency of 1...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr401181y

    authors： Dickhut C,Feldmann I,Lambert J,Zahedi RP

    更新日期：2014-06-06 00:00:00

  • Quality Control and Outlier Detection of Targeted Mass Spectrometry Data from Multiplex Protein Panels.

    abstract：:Increased throughput as well as increased multiplexing of liquid chromatography coupled to selected reaction monitoring mass spectrometry (LC-SRM-MS) assays for protein quantification challenges routine data analysis. Despite the measurement of multiple transitions from multiple peptides, for clinical applications a s...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/acs.jproteome.9b00854

    authors： van den Broek I,Mastali M,Mouapi K,Bystrom C,Bairey Merz CN,Van Eyk JE

    更新日期：2020-06-05 00:00:00

  • Common glycoproteins expressing polylactosamine-type glycans on matched patient primary and metastatic melanoma cells show different glycan profiles.

    abstract：:Recently, we reported comparative analysis of glycoproteins which express cancer-specific N-glycans on various cancer cells and identified 24 glycoproteins having polylactosamine (polyLacNAc)-type N-glycans that are abundantly present in malignant cells [ Mitsui et al., J. Pharm. Biomed. Anal. 2012 , 70 , 718 - 726 ]....

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr401015b

    authors： Kinoshita M,Mitsui Y,Kakoi N,Yamada K,Hayakawa T,Kakehi K

    更新日期：2014-02-07 00:00:00

  • Proteome characterization of human NK-92 cells identifies novel IFN-alpha and IL-15 target genes.

    abstract：:Natural killer (NK) cells are important components of innate immune defense. NK cells kill virus-infected cells and secrete cytokines that are involved in activation of other immune cells. Macrophage-derived cytokines interferon-alpha (IFN-alpha) and interleukin-15 (IL-15) are in turn important activators of NK cells,...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr049857b

    authors： Rakkola R,Matikainen S,Nyman TA

    更新日期：2005-01-01 00:00:00

  • Comparative Proteomics of Contrasting Maize Genotypes Provides Insights into Salt-Stress Tolerance Mechanisms.

    abstract：:Salt stress is a major abiotic factor limiting maize yield. To characterize the mechanism underlying maize salt tolerance, we compared the seedling root proteomes of salt-tolerant Jing724 and salt-sensitive D9H. The germination rate and growth parameter values (weight and length) were higher for Jing724 than for D9H u...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/acs.jproteome.7b00455

    authors： Luo M,Zhao Y,Wang Y,Shi Z,Zhang P,Zhang Y,Song W,Zhao J

    更新日期：2018-01-05 00:00:00

  • Correlation of phenotypic profiles using targeted proteomics identifies mycobacterial esx-1 substrates.

    abstract：:The Esx/WXG-100 (ESAT-6/Wss) exporters are multiprotein complexes that promote protein translocation across the cytoplasmic membrane in a diverse range of pathogenic and nonpathogenic bacterial species. The Esx-1 (ESAT-6 System-1) system mediates virulence factor translocation in mycobacterial pathogens, including the...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr500484w

    authors： Champion MM,Williams EA,Pinapati RS,Champion PA

    更新日期：2014-11-07 00:00:00

  • Optimization of Search Engines and Postprocessing Approaches to Maximize Peptide and Protein Identification for High-Resolution Mass Data.

    abstract：:The two key steps for analyzing proteomic data generated by high-resolution MS are database searching and postprocessing. While the two steps are interrelated, studies on their combinatory effects and the optimization of these procedures have not been adequately conducted. Here, we investigated the performance of thre...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/acs.jproteome.5b00536

    authors： Tu C,Sheng Q,Li J,Ma D,Shen X,Wang X,Shyr Y,Yi Z,Qu J

    更新日期：2015-11-06 00:00:00

  • Proteome analysis of a hepatocyte-specific BIRC5 (survivin)-knockout mouse model during liver regeneration.

    abstract：:The Baculoviral IAP repeat-containing protein 5 (BIRC5), also known as inhibitor of apoptosis protein survivin, is a member of the chromosomal passenger complex and a key player in mitosis. To investigate the function of BIRC5 in liver regeneration, we analyzed a hepatocyte-specific BIRC5-knockout mouse model using a ...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr401188r

    authors： Bracht T,Hagemann S,Loscha M,Megger DA,Padden J,Eisenacher M,Kuhlmann K,Meyer HE,Baba HA,Sitek B

    更新日期：2014-06-06 00:00:00

  • Validation of endogenous peptide identifications using a database of tandem mass spectra.

    abstract：:The SwePep database is designed for endogenous peptides and mass spectrometry. It contains information about the peptides such as mass, pl, precursor protein and potential post-translational modifications. Here, we have improved and extended the SwePep database with tandem mass spectra, by adding a locally curated ver...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr800036d

    authors： Fälth M,Svensson M,Nilsson A,Sköld K,Fenyö D,Andren PE

    更新日期：2008-07-01 00:00:00

  • We are what we eat: food safety and proteomics.

    abstract：:In this review, we lead the reader through the evolution of proteomics application to the study of quality control in production processes of foods (including food of plant origin and transgenic plants in particular, but also meat, wine and beer, and milk) and food safety (screening for foodborne pathogens). These top...

    journal_title：Journal of proteome research

    pub_type： 杂志文章,评审

    doi：10.1021/pr2008829

    authors： D'Alessandro A,Zolla L

    更新日期：2012-01-01 00:00:00

  • Compartment proteomics analysis of white perch (Morone americana) ovary using support vector machines.

    abstract：:Compartment proteomics enable broad characterization of target tissues. We employed a simple fractionation method and filter-aided sample preparation (FASP) to characterize the cytosolic and membrane fractions of white perch ovary tissues by semiquantitative tandem mass spectrometry using label-free quantitation based...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr401067g

    authors： Schilling J,Nepomuceno A,Schaff JE,Muddiman DC,Daniels HV,Reading BJ

    更新日期：2014-03-07 00:00:00

  • Phosphoproteomic analysis of human mesenchymal stromal cells during osteogenic differentiation.

    abstract：:Human mesenchymal stromal cells (hMSCs) are promising candidates for cell therapy and tissue regeneration. Knowledge of the molecular mechanisms governing hMSC commitment into osteoblasts is critical to the development of therapeutic applications for human bone diseases. Because protein phosphorylation plays a critica...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr200868p

    authors： Lo T,Tsai CF,Shih YR,Wang YT,Lu SC,Sung TY,Hsu WL,Chen YJ,Lee OK

    更新日期：2012-02-03 00:00:00

  • Biochemical and proteomic analysis of a potential anticancer agent: Palladium(II) Saccharinate complex of terpyridine acting through double strand break formation.

    abstract：:Metal based chemotherapeutic drugs are widely used as an effective method to defeat various cancers. In this study, the mechanism of action of a novel therapeutic agent, [Pd(sac)(terpy)](sac)·4H2O (sac = saccharinate, and terpy = 2,2':6',2″-terpyridine) was studied. To better understand the proteomic changes in respon...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr5006718

    authors： Adiguzel Z,Baykal AT,Kacar O,Yilmaz VT,Ulukaya E,Acilan C

    更新日期：2014-11-07 00:00:00

  • Proteomic analysis of multiprotein complexes in the thylakoid membrane upon cadmium treatment.

    abstract：:The time course of the thylakoid membranes proteomic profile changes upon cadmium (Cd) addition to hydroponic Spinacia oleracea L. plants has been investigated. Two different proteomic approaches have been used: blue native gel electrophoresis followed by SDS-PAGE (2D BN-SDS-PAGE) and sucrose density gradient ultracen...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr800507x

    authors： Fagioni M,D'Amici GM,Timperio AM,Zolla L

    更新日期：2009-01-01 00:00:00

  • Proteomic characteristics of ex vivo-enriched adult human bone marrow mononuclear cells in continuous perfusion cultures.

    abstract：:A major challenge in developing cell therapies is reliable characterization of the cell product at the molecular level. Fresh autologous and passaged human bone marrow enriched for stem and mesenchymal stromal stem cells have been used to regenerate bone. We report the proteome of an innovative autologous human bone m...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr801064u

    authors： Prahalad AK,Hock JM

    更新日期：2009-04-01 00:00:00

  • A limited screen for protein interactions reveals new roles for protein phosphatase 1 in cell cycle control and apoptosis.

    abstract：:Protein phosphatase 1 (PP1) catalytic subunits typically combine with other proteins that modulate their activity, direct them to distinct substrates, or serve as substrates for PP1. More than 50 PP1-interacting proteins (PIPs) have been identified so far. Given there are approximately 10 000 phosphoproteins in mammal...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr060504h

    authors： Flores-Delgado G,Liu CW,Sposto R,Berndt N

    更新日期：2007-03-01 00:00:00