AKT1 and AKT2 induce distinct phosphorylation patterns in HL-1 cardiac myocytes.


:The protein kinase AKT is a central kinase in the heart and has a major impact on growth/hypertrophy, survival/apoptosis, and metabolism. To gain more insight into AKT isoform-specific signaling at the molecular level, we investigated the phosphoproteome of HL-1 cardiomyocytes carrying AKT1 or AKT2 isoform-specific knock down, respectively. We combined stable isotope labeling with high resolution mass spectrometry and identified 377 regulated phosphopeptides. Although AKT1 is expressed at 4-fold higher levels, insulin stimulation mainly activated AKT2, which might in part rely on a preferred interaction of AKT2 with the mammalian target of rapamycin complex 2. In line with this result, the highest number of regulated phosphopeptides was identified in the AKT2 knock down cells. Isoform-specific regulation of AKT targets not previously described could be observed, and specific regulation of indirect target sites allows a deeper insight into affected biological processes. In the myocardial context, we identified many phosphosites supporting a connection of AKT to excitation-contraction coupling. Phosphoproteins identified included L-type calcium channel, ryanodine receptor, junctophilin, histidine-rich calcium binding protein, phospholamban, heat shock protein beta-6, and Ca²⁺/calmodulin-dependent kinase II. In conclusion, AKT isoform-specific knock down combined with quantitative phosphoproteomics provided a powerful strategy to unravel AKT isoform-specific signaling.


J Proteome Res


Reinartz M,Raupach A,Kaisers W,Gödecke A




Has Abstract


2014-10-03 00:00:00












  • Serum Antibody Profile during Colonization of the Mouse Gut by Candida albicans: Relevance for Protection during Systemic Infection.

    abstract::Candida albicans is a commensal microorganism in the oral cavity and gastrointestinal and urogenital tracts of most individuals that acts as an opportunistic pathogen when the host immune response is reduced. Here, we established different immunocompetent murine models to analyze the antibody responses to the C. albic...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Huertas B,Prieto D,Pitarch A,Gil C,Pla J,Díez-Orejas R

    更新日期:2017-01-06 00:00:00

  • Impact of digestion conditions on phosphoproteomics.

    abstract::In the past few years, the focus of phosphoproteomics has shifted from merely qualitative to quantitative and targeted studies. Tryptic digestion is a critical step that directly affects quantification and that can be impaired by phosphorylation. Therefore, we systematically characterized the digestion efficiency of 1...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Dickhut C,Feldmann I,Lambert J,Zahedi RP

    更新日期:2014-06-06 00:00:00

  • Sonoreactor-based technology for fast high-throughput proteolytic digestion of proteins.

    abstract::Fast (120 s) and high-throughput (more than six samples at once) in-gel trypsin digestion of proteins using sonoreactor technology has been achieved. Successful protein identification was done by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, MALDI-TOF-MS. Specific identification of the ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Rial-Otero R,Carreira RJ,Cordeiro FM,Moro AJ,Fernandes L,Moura I,Capelo JL

    更新日期:2007-02-01 00:00:00

  • Comparative proteome cataloging of Lactobacillus rhamnosus strains GG and Lc705.

    abstract::The present study reports an in-depth proteome analysis of two Lactobacillus rhamnosus strains, the well-known probiotic strain GG and the dairy strain Lc705. We used GeLC-MS/MS, in which proteins are separated using 1-DE and identified using nanoLC-MS/MS, to generate high-quality protein catalogs. To maximize the num...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Savijoki K,Lietzén N,Kankainen M,Alatossava T,Koskenniemi K,Varmanen P,Nyman TA

    更新日期:2011-08-05 00:00:00

  • Proteomic analysis of left ventricular remodeling in an experimental model of heart failure.

    abstract::The development of chronic heart failure (CHF) following myocardial infarction is characterized by progressive alterations of left ventricle (LV) structure and function called left ventricular remodeling (LVR), but the mechanism of LVR remains still unclear. Moreover, information concerning the global alteration prote...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Cieniewski-Bernard C,Mulder P,Henry JP,Drobecq H,Dubois E,Pottiez G,Thuillez C,Amouyel P,Richard V,Pinet F

    更新日期:2008-11-01 00:00:00

  • Integrity of Glycosylation Processing of a Glycan-Depleted Trimeric HIV-1 Immunogen Targeting Key B-Cell Lineages.

    abstract::Broadly neutralizing antibodies (bNAbs) that target the trimeric HIV-1 envelope glycoprotein spike (Env) are tools that can guide the design of recombinant Env proteins intended to engage the predicted human germline precursors of bNAbs (gl-bNAbs). The protein components of gl-bNAb epitopes are often masked by glycans...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Behrens AJ,Kumar A,Medina-Ramirez M,Cupo A,Marshall K,Cruz Portillo VM,Harvey DJ,Ozorowski G,Zitzmann N,Wilson IA,Ward AB,Struwe WB,Moore JP,Sanders RW,Crispin M

    更新日期:2018-03-02 00:00:00

  • Alterations in the Glycan Profile of Mouse Transferrin: New Insights in Collagen-Induced Arthritis.

    abstract::Transferrin purification from mice serum samples by immunoaffinity chromatography (IAC) was optimized in order to study the possible modifications occurring in its glycans in collagen-induced arthritis (CIA) samples. SDS-PAGE and nanoLC-MS/MS were used to monitor the IAC purification performance. Afterward, a relative...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Mancera-Arteu M,Giménez E,Sancho J,Sanz-Nebot V

    更新日期:2020-04-03 00:00:00

  • Lipid Profiling in Epicardial and Subcutaneous Adipose Tissue of Patients with Coronary Artery Disease.

    abstract::Coronary artery disease is one of the most frequent causes of morbidity and mortality worldwide. It is even more prevalent in patients with type 2 diabetes mellitus who suffer from obesity and increased accumulation of epicardial fat with a possible contributing role in the development of coronary artery disease. We p...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Tomášová P,Čermáková M,Pelantová H,Vecka M,Kratochvílová H,Lipš M,Lindner J,Ivák P,Netuka I,Šedivá B,Haluzík M,Kuzma M

    更新日期:2020-10-02 00:00:00

  • Direct protein detection from biological media through electrospray-assisted laser desorption ionization/mass spectrometry.

    abstract::We report here using a novel technology-electrospray-assisted laser desorption ionization (ELDI)/mass spectrometry-for the rapid and sensitive detection of the major proteins that exist in dried biological fluids (e.g., blood, tears, saliva, serum), bacterial cultures, and tissues (e.g., porcine liver and heart) under...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Huang MZ,Hsu HJ,Lee JY,Jeng J,Shiea J

    更新日期:2006-05-01 00:00:00

  • Proteomic identification of in vivo interactors reveals novel function of skin cornification proteins.

    abstract::Protection against injurious external insults and loss of vital fluids is essential for life and is in all organisms, from bacteria to plants and humans, provided by some form of barrier. Members of the small proline-rich (SPRR) protein family are major components of the cornified cell envelope (CE), a structure respo...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Vermeij WP,Florea BI,Isenia S,Alia A,Brouwer J,Backendorf C

    更新日期:2012-06-01 00:00:00

  • Radioactive Chernobyl environment has produced high-oil flax seeds that show proteome alterations related to carbon metabolism during seed development.

    abstract::Starting in 2007, we have grown soybean (Glycine max [L.] Merr. variety Soniachna) and flax (Linum usitatissimum, L. variety Kyivskyi) in the radio-contaminated Chernobyl area and analyzed the seed proteomes. In the second-generation flax seeds, we detected a 12% increase in oil content. To characterize the bases for ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Klubicová K,Danchenko M,Skultety L,Berezhna VV,Rashydov NM,Hajduch M

    更新日期:2013-11-01 00:00:00

  • Circulating Lamin B1 (LMNB1) biomarker detects early stages of liver cancer in patients.

    abstract::Hepatocellular carcinoma (HCC) is a major liver malignancy possessing a high mortality rate and is particularly prevalent in China and Asia. While surgery is the most effective treatment for liver tumor, about 80% of HCC patients are inoperable at presentation and die early due to late diagnosis. For early cancer dete...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Sun S,Xu MZ,Poon RT,Day PJ,Luk JM

    更新日期:2010-01-01 00:00:00

  • Comparative proteomics implicates a role for multiple secretion systems in electrode-respiring Geobacter sulfurreducens biofilms.

    abstract::Geobacter sulfurreducens is a dissimilatory metal-reducing bacterium capable of forming thick electron-conducting biofilms on solid electrodes. Here, we employ for the first time comparative proteomics to identify key physiological changes involved in G. sulfurreducens adaptation from fumarate-respiring planktonic cel...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Kavanagh P,Botting CH,Jana PS,Leech D,Abram F

    更新日期:2016-01-01 00:00:00

  • High-affinity recombinant antibody fragments (Fabs) can be applied in peptide enrichment immuno-MRM assays.

    abstract::High-affinity antibodies binding to linear peptides in solution are a prerequisite for performing immuno-MRM, an emerging technology for protein quantitation with high precision and specificity using peptide immunoaffinity enrichment coupled to stable isotope dilution and targeted mass spectrometry. Recombinant antibo...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Whiteaker JR,Zhao L,Frisch C,Ylera F,Harth S,Knappik A,Paulovich AG

    更新日期:2014-04-04 00:00:00

  • "Differential Visual Proteomics": Enabling the Proteome-Wide Comparison of Protein Structures of Single-Cells.

    abstract::Proteins are involved in all tasks of life, and their characterization is essential to understand the underlying mechanisms of biological processes. We present a method called "differential visual proteomics" geared to study proteome-wide structural changes of proteins and protein-complexes between a disturbed and an ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Syntychaki A,Rima L,Schmidli C,Stohler T,Bieri A,Sütterlin R,Stahlberg H,Castaño-Díez D,Braun T

    更新日期:2019-09-06 00:00:00

  • Sputum proteomics identifies elevated PIGR levels in smokers and mild-to-moderate COPD.

    abstract::Chronic obstructive pulmonary disease (COPD) is one of the leading causes of morbidity and mortality around the world. However, the exact mechanisms leading to COPD and its progression are still poorly understood. In this study, induced sputum was analyzed by cysteine-specific two-dimensional difference gel electropho...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Ohlmeier S,Mazur W,Linja-Aho A,Louhelainen N,Rönty M,Toljamo T,Bergmann U,Kinnula VL

    更新日期:2012-02-03 00:00:00

  • Glycomic analyses of glycoproteins in bile and serum during rat hepatocarcinogenesis.

    abstract::Fucosylated alpha-fetoprotein (AFP) is a specific tumor marker for hepatocellular carcinomas (HCC). However, the mechanisms underlying the increase in fucosylated AFP in serum of patients with HCC are largely unknown. Recently, we reported that fucosylation is a possible signal for the secretion of glycoproteins into ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Nakagawa T,Takeishi S,Kameyama A,Yagi H,Yoshioka T,Moriwaki K,Masuda T,Matsumoto H,Kato K,Narimatsu H,Taniguchi N,Miyoshi E

    更新日期:2010-10-01 00:00:00

  • Vimentin is a functional partner of hormone sensitive lipase and facilitates lipolysis.

    abstract::Lipolysis involves a number of components including signaling pathways, droplet-associated proteins, and lipases such as hormone-sensitive lipase (HSL). We used surface enhanced laser desorption/ionization time-of-flight mass spectroscopy to identify cellular proteins that might interact with HSL and potentially influ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Shen WJ,Patel S,Eriksson JE,Kraemer FB

    更新日期:2010-04-05 00:00:00

  • Selective DOT1L, LSD1, and HDAC Class I Inhibitors Reduce HOXA9 Expression in MLL-AF9 Rearranged Leukemia Cells, But Dysregulate the Expression of Many Histone-Modifying Enzymes.

    abstract::Mixed lineage leukemia results from chromosomal rearrangements of the gene mixed lineage leukemia (MLL). MLL-AF9 is one such rearrangement that recruits the lysine methyltransferase, human disruptor of telomere silencing 1-like (DOT1L) and lysine specific demethylase 1 (LSD1), resulting in elevated expression of the H...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Lillico R,Lawrence CK,Lakowski TM

    更新日期:2018-08-03 00:00:00

  • Glycoproteomic analysis of glioblastoma stem cell differentiation.

    abstract::Cancer stem cells are responsible for tumor formation through self-renewal and differentiation into multiple cell types and thus represent a new therapeutic target for tumors. Glycoproteins play a critical role in determining the fates of stem cells such as self-renewal, proliferation, and differentiation. Here we app...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: He J,Liu Y,Zhu TS,Xie X,Costello MA,Talsma CE,Flack CG,Crowley JG,Dimeco F,Vescovi AL,Fan X,Lubman DM

    更新日期:2011-01-07 00:00:00

  • Insights from Chromosome-Centric Mapping of Disease-Associated Genes: Chromosome 12 Perspective.

    abstract::In line with the aims of the Chromosome-based Human Proteome Project and the Biology/Disease-based Human Proteome Project, we have been studying differentially expressed transcripts and proteins in gliomas—the most prevalent primary brain tumors. Here, we present a perspective on important insights from this analysis ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Jayaram S,Gupta MK,Shivakumar BM,Ghatge M,Sharma A,Vangala RK,Sirdeshmukh R

    更新日期:2015-09-04 00:00:00

  • Citrate boosts the performance of phosphopeptide analysis by UPLC-ESI-MS/MS.

    abstract::Incomplete recovery from the LC column is identified as a major cause for poor detection efficiency of phosphopeptides by LC-MS/MS. It is proposed that metal ions adsorbed on the stationary phase interact with the phosphate group of phosphopeptides via an ion-pairing mechanism related to IMAC (IMAC: immobilized metal ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Winter D,Seidler J,Ziv Y,Shiloh Y,Lehmann WD

    更新日期:2009-01-01 00:00:00

  • Metabolomic Profiling of the Aqueous Humor in Patients with Wet Age-Related Macular Degeneration Using UHPLC-MS/MS.

    abstract::Assessing metabolomic alterations in age-related macular degeneration (AMD) can provide insights into its pathogenesis. We compared the metabolomic profiles of the aqueous humor between wet AMD patients (n = 26) and age- and sex-matched patients undergoing cataract surgery without AMD as controls (n = 20). A global un...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Han G,Wei P,He M,Teng H,Chu Y

    更新日期:2020-06-05 00:00:00

  • Different residues in channel turret determining the selectivity of ADWX-1 inhibitor peptide between Kv1.1 and Kv1.3 channels.

    abstract::The low selectivity of Kv1 peptide inhibitors for specific isoforms makes them poor candidates for the development of theraputics. Using combined approaches, we showed that the Kv1 turret is the critical determinant for ADWX-1 peptide inhibitor selectivity of Kv1.3 over Kv1.1. Mutation of Kv1.1 turret residues to matc...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Yin SJ,Jiang L,Yi H,Han S,Yang DW,Liu ML,Liu H,Cao ZJ,Wu YL,Li WX

    更新日期:2008-11-01 00:00:00

  • Proteomic Characterization of Epithelial-Like Extracellular Vesicles in Advanced Endometrial Cancer.

    abstract::Endometrial cancer (EC) is the most frequent gynecological cancer. Tumor dissemination affecting ∼20% of EC patients is characterized at the primary carcinoma by epithelial-to-mesenchymal transition (EMT) associated with myometrial infiltration. At distant sites, the interaction of circulating tumor cells (CTCs) with ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Mariscal J,Fernandez-Puente P,Calamia V,Abalo A,Santacana M,Matias-Guiu X,Lopez-Lopez R,Gil-Moreno A,Alonso-Alconada L,Abal M

    更新日期:2019-03-01 00:00:00

  • Model for Quality Control of Allergen Products with Mass Spectrometry.

    abstract::Birch pollen allergy is diagnosed and treated with aqueous extracts from birch pollen, which contain a mixture of allergens and nonallergenic proteins, including large numbers of closely related sequence variants, so-called iso-allergens of the major allergen, Bet v 1. The quality of therapeutic and diagnostic allerge...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Spiric J,Schulenborg T,Schwaben L,Engin AM,Karas M,Reuter A

    更新日期:2017-10-06 00:00:00

  • B. subtilis ribosomal proteins: structural homology and post-translational modifications.

    abstract::Ribosomal proteins of the model gram-positive bacterium B. subtilis 168 were extensively characterized in a proteomic study. Mass spectra of the 52 proteins expected to be constitutive components of the 70S ribosome were recorded. Peptide MS/MS analysis with an average sequence coverage of 85% supported the identifica...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Lauber MA,Running WE,Reilly JP

    更新日期:2009-09-01 00:00:00

  • Challenges in Peptide-Spectrum Matching: A Robust and Reproducible Statistical Framework for Removing Low-Accuracy, High-Scoring Hits.

    abstract::Workflows for large-scale (MS)-based shotgun proteomics can potentially lead to costly errors in the form of incorrect peptide-spectrum matches (PSMs). To improve the robustness of these workflows, we have investigated the use of the precursor mass discrepancy (PMD) to detect and filter potentially false PSMs that hav...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Hubler SL,Kumar P,Mehta S,Easterly C,Johnson JE,Jagtap PD,Griffin TJ

    更新日期:2020-01-03 00:00:00

  • Modulation of colon cancer by nutmeg.

    abstract::Colon cancer is the most common cancer and the third leading cause of cancer mortality in humans. Using mass spectrometry-based metabolomics, the current study revealed the accumulation of four uremic toxins (cresol sulfate, cresol glucuronide, indoxyl sulfate, and phenyl sulfate) in the serum of mice harboring adenom...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Li F,Yang XW,Krausz KW,Nichols RG,Xu W,Patterson AD,Gonzalez FJ

    更新日期:2015-04-03 00:00:00

  • On the development of plasma protein biomarkers.

    abstract::The development of plasma biomarkers has proven to be more challenging than initially anticipated. Many studies have reported lists of candidate proteins rather than validated candidate markers with an assigned performance to a specific clinical objective. Biomarker research necessitates a clear rational framework wit...

    journal_title:Journal of proteome research

    pub_type: 杂志文章,评审


    authors: Surinova S,Schiess R,Hüttenhain R,Cerciello F,Wollscheid B,Aebersold R

    更新日期:2011-01-07 00:00:00