Abstract:
:Advances in mass spectrometry have made the quantitative measurement of proteins across multiple samples a reality, allowing for the study of complex biological systems such as the metabolic syndrome. Although the deregulation of lipid metabolism and increased hepatic storage of triacylglycerides are known to play a part in the onset of the metabolic syndrome, its molecular basis and dependency on dietary and genotypic factors are poorly characterized. Here, we used an experimental design with two different mouse strains and dietary and metabolic perturbations to generate a compendium of quantitative proteome data using three mass spectrometric techniques. The data reproduce known properties of the metabolic system and indicate differential molecular adaptation of the two mouse strains to perturbations, contributing to a better understanding of the metabolic syndrome. We show that high-quality, high-throughput proteomic data sets provide an unbiased broad overview of the behavior of complex systems after perturbation.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Terfve C,Sabidó E,Wu Y,Gonçalves E,Choi M,Vaga S,Vitek O,Saez-Rodriguez J,Aebersold Rdoi
10.1021/acs.jproteome.6b00815subject
Has Abstractpub_date
2017-02-03 00:00:00pages
831-841issue
2eissn
1535-3893issn
1535-3907journal_volume
16pub_type
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