Protein array based interactome analysis of amyloid-β indicates an inhibition of protein translation.

abstract：:Reference materials are vital to benchmarking the reproducibility of clinical tests and essential for monitoring laboratory performance for clinical proteomics. The reference material utilized for mass spectrometric analysis of the human proteome would ideally contain enough proteins to be suitably representative of t...

journal_title：Journal of proteome research

authors： Clark DJ,Hu Y,Bocik W,Chen L,Schnaubelt M,Roberts R,Shah P,Whiteley G,Zhang H

更新日期：2018-06-01 00:00:00

abstract：:Compartment proteomics enable broad characterization of target tissues. We employed a simple fractionation method and filter-aided sample preparation (FASP) to characterize the cytosolic and membrane fractions of white perch ovary tissues by semiquantitative tandem mass spectrometry using label-free quantitation based...

journal_title：Journal of proteome research

authors： Schilling J,Nepomuceno A,Schaff JE,Muddiman DC,Daniels HV,Reading BJ

更新日期：2014-03-07 00:00:00

abstract：:Phospholipase C gamma 1 (PLC-γ1) occupies a critically important position in the T-cell signaling pathway. While its functions as a regulator of both Ca2+ signaling and PKC-family kinases are well characterized, PLC-γ1's role in the regulation of early T-cell receptor signaling events is incompletely understood. Activ...

journal_title：Journal of proteome research

authors： Belmont J,Gu T,Mudd A,Salomon AR

更新日期：2017-08-04 00:00:00

abstract：:Glycosylation is an abundant and important protein modification with large influence on the properties and interactions of glycoconjugates. Human plasma N-glycosylation has been the subject of frequent investigation, revealing strong associations with physiological and pathological conditions. Less well-characterized ...

journal_title：Journal of proteome research

authors： Reiding KR,Hipgrave Ederveen AL,Rombouts Y,Wuhrer M

更新日期：2016-10-07 00:00:00

abstract：:A comprehensive study of the molecular active landscape of human cells can be undertaken to integrate two different but complementary perspectives: transcriptomics, and proteomics. After the genome era, proteomics has emerged as a powerful tool to simultaneously identify and characterize the compendium of thousands of...

journal_title：Journal of proteome research

authors： Díez P,Droste C,Dégano RM,González-Muñoz M,Ibarrola N,Pérez-Andrés M,Garin-Muga A,Segura V,Marko-Varga G,LaBaer J,Orfao A,Corrales FJ,De Las Rivas J,Fuentes M

更新日期：2015-09-04 00:00:00

abstract：:Flooding has severe negative effects on soybean growth. To explore the flooding-responsive mechanisms in early-stage soybean, a phosphoproteomic approach was used. Two-day-old soybean plants were treated without or with flooding for 3, 6, 12, and 24 h, and root tip proteins were then extracted and analyzed at each tim...

journal_title：Journal of proteome research

authors： Yin X,Sakata K,Komatsu S

更新日期：2014-12-05 00:00:00

abstract：:The vast majority of human multiexon genes undergo alternative splicing and produce a variety of splice variant transcripts and proteins, which can perform different functions. These protein-coding splice variants (PCSVs) greatly increase the functional diversity of proteins. Most functional annotation algorithms have...

journal_title：Journal of proteome research

authors： Panwar B,Menon R,Eksi R,Li HD,Omenn GS,Guan Y

更新日期：2016-06-03 00:00:00

abstract：:The plant cell wall is a dynamic cellular compartment consisting of a complex matrix of components that can change dramatically in response to environmental stresses. During pathogen attack, for instance, a wide spectrum of proteins that participate in various sequential processes involved in plant defense is secreted...

journal_title：Journal of proteome research

authors： Cheng FY,Blackburn K,Lin YM,Goshe MB,Williamson JD

更新日期：2009-01-01 00:00:00

abstract：:Flooding is a serious problem for soybeans because it reduces growth and grain yield. Proteomic and metabolomic techniques were used to examine whether mitochondrial function is altered in soybeans by flooding stress. Mitochondrial fractions were purified from the roots and hypocotyls of 4-day-old soybean seedlings th...

journal_title：Journal of proteome research

authors： Komatsu S,Yamamoto A,Nakamura T,Nouri MZ,Nanjo Y,Nishizawa K,Furukawa K

更新日期：2011-09-02 00:00:00

abstract：:Royal jelly (RJ) is an exclusive food for queen honey bee (Apis mellifera L.) that is synthesized and secreted by young worker bees. RJ is also widely used in medical products, cosmetics, and as health foods. However, little is known about RJ functionality and the total protein components, although recent research is ...

journal_title：Journal of proteome research

authors： Furusawa T,Rakwal R,Nam HW,Shibato J,Agrawal GK,Kim YS,Ogawa Y,Yoshida Y,Kouzuma Y,Masuo Y,Yonekura M

更新日期：2008-08-01 00:00:00

abstract：:Ion mobility-mass spectrometry (IM-MS) is a useful technique for determining information about analyte ion conformation in addition to mass/charge ratio. The physical principles that govern the mobility of an ion through a gas in the presence of a uniform electric field are well understood, enabling rotationally avera...

journal_title：Journal of proteome research

authors： Chawner R,McCullough B,Giles K,Barran PE,Gaskell SJ,Eyers CE

更新日期：2012-11-02 00:00:00

abstract：:Protein phosphorylation is the most abundant reversible covalent modification. Human protein kinases participate in almost all biological pathways, and approximately half of the kinases are associated with disease. PhoSigNet was designed to store and display human phosphorylation-mediated signal transduction networks,...

journal_title：Journal of proteome research

authors： Zhang M,Li H,He Y,Sun H,Xia L,Wang L,Sun B,Ma L,Zhang G,Li J,Li Y,Xie L

更新日期：2015-07-02 00:00:00

abstract：:Birch pollen allergy is diagnosed and treated with aqueous extracts from birch pollen, which contain a mixture of allergens and nonallergenic proteins, including large numbers of closely related sequence variants, so-called iso-allergens of the major allergen, Bet v 1. The quality of therapeutic and diagnostic allerge...

journal_title：Journal of proteome research

authors： Spiric J,Schulenborg T,Schwaben L,Engin AM,Karas M,Reuter A

更新日期：2017-10-06 00:00:00

abstract：:Modeling and simulation of biological networks is an effective and widely used research methodology. The Biological Network Simulator (BioNSi) is a tool for modeling biological networks and simulating their discrete-time dynamics, implemented as a Cytoscape App. BioNSi includes a visual representation of the network t...

journal_title：Journal of proteome research

authors： Rubinstein A,Bracha N,Rudner L,Zucker N,Sloin HE,Chor B

更新日期：2016-08-05 00:00:00

abstract：:Decoy database searches are used to filter out false positive protein identifications derived from search engines, but there is no consensus about which decoy is "the best". We evaluate nine different decoy designs using public data sets from samples of known composition. Statistically significant performance differen...

journal_title：Journal of proteome research

authors： Blanco L,Mead JA,Bessant C

更新日期：2009-04-01 00:00:00

abstract：:The Mycobacterium tuberculosis membrane is rich in antigens that are potential targets for diagnostics and the development of new vaccines. To better understand the mechanisms underlying MTB virulence and identify new targets for therapeutic intervention, we investigated the differential composition of membrane proteo...

journal_title：Journal of proteome research

authors： Gunawardena HP,Feltcher ME,Wrobel JA,Gu S,Braunstein M,Chen X

更新日期：2013-12-06 00:00:00

abstract：:The physical, endocrine, and metabolic responses of livestock to road transport have been evaluated by conventional hematological and biochemistry parameters for more than 20 years. However, these measures are relatively insensitive to subtle metabolic adaptations. We applied NMR-based metabonomics to assess system-wi...

journal_title：Journal of proteome research

authors： Li J,Wijffels G,Yu Y,Nielsen LK,Niemeyer DO,Fisher AD,Ferguson DM,Schirra HJ

更新日期：2011-03-04 00:00:00

abstract：:The Baculoviral IAP repeat-containing protein 5 (BIRC5), also known as inhibitor of apoptosis protein survivin, is a member of the chromosomal passenger complex and a key player in mitosis. To investigate the function of BIRC5 in liver regeneration, we analyzed a hepatocyte-specific BIRC5-knockout mouse model using a ...

journal_title：Journal of proteome research

authors： Bracht T,Hagemann S,Loscha M,Megger DA,Padden J,Eisenacher M,Kuhlmann K,Meyer HE,Baba HA,Sitek B

更新日期：2014-06-06 00:00:00

abstract：:Histone post-translational modifications (PTMs) are fundamental players of chromatin regulation, as they contribute to editing histone chemical properties and recruiting proteins for gene transcription and DNA repair. Mass spectrometry (MS)-based proteomics is currently the most widely adopted strategy for high-throug...

journal_title：Journal of proteome research

authors： Guo Q,Sidoli S,Garcia BA,Zhao X

更新日期：2018-01-05 00:00:00

abstract：:Cancers are initiated and developed from a small population of stem-like cells termed cancer stem cells (CSCs). There is heterogeneity among this CSC population that leads to multiple subpopulations with their own distinct biological features and protein expression. The protein expression and function may be impacted ...

journal_title：Journal of proteome research

authors： Tan Z,Nie S,McDermott SP,Wicha MS,Lubman DM

更新日期：2017-02-03 00:00:00

abstract：:Human saliva is a protein-rich, easily accessible source of potential local and systemic biomarkers to monitor changes that occur under pathological conditions; however, little is known about the changes in abundance associated with normal aging. In this study, we performed a comprehensive proteomic profiling of poole...

journal_title：Journal of proteome research

authors： Ambatipudi KS,Lu B,Hagen FK,Melvin JE,Yates JR

更新日期：2009-11-01 00:00:00

abstract：:Histones regulate a variety of chromatin templated events by their post-translational modifications (PTMs). Although there are extensive reports on the PTMs of canonical histones, the information on the histone variants remains very scanty. Here, we report the identification of different PTMs, such as acetylation, met...

journal_title：Journal of proteome research

authors： Pentakota SK,Sandhya S,P Sikarwar A,Chandra N,Satyanarayana Rao MR

更新日期：2014-12-05 00:00:00

abstract：:Coronary artery disease remains a leading cause of death in industrialized nations, and early detection of disease is a critical intervention target to effectively treat patients and manage risk. Proteomic analysis of mixed tissue homogenates may obscure subtle protein changes that occur uniquely in underlying tissue ...

journal_title：Journal of proteome research

authors： Parker SJ,Chen L,Spivia W,Saylor G,Mao C,Venkatraman V,Holewinski RJ,Mastali M,Pandey R,Athas G,Yu G,Fu Q,Troxlair D,Vander Heide R,Herrington D,Van Eyk JE,Wang Y

更新日期：2020-07-02 00:00:00

abstract：:Proteoforms containing post-translational modifications (PTMs) represent a degree of functional diversity only harnessed through analytically precise simultaneous quantification of multiple PTMs. Here we present a method to accurately differentiate an unmodified peptide from its PTM-containing counterpart through data...

journal_title：Journal of proteome research

authors： Robinson AE,Binek A,Venkatraman V,Searle BC,Holewinski RJ,Rosenberger G,Parker SJ,Basisty N,Xie X,Lund PJ,Saxena G,Mato JM,Garcia BA,Schilling B,Lu SC,Van Eyk JE

更新日期：2020-10-02 00:00:00

abstract：:The characterization of the humoral response in Alzheimer's disease (AD) patients might aid in detecting the disease at early stages. We have combined phage display and protein microarrays to identify AD autoantibodies and their target biomarkers. After enrichment of the T7 phage display libraries from AD and healthy ...

journal_title：Journal of proteome research

authors： San Segundo-Acosta P,Montero-Calle A,Fuentes M,Rábano A,Villalba M,Barderas R

更新日期：2019-07-05 00:00:00

abstract：:Lipolysis involves a number of components including signaling pathways, droplet-associated proteins, and lipases such as hormone-sensitive lipase (HSL). We used surface enhanced laser desorption/ionization time-of-flight mass spectroscopy to identify cellular proteins that might interact with HSL and potentially influ...

journal_title：Journal of proteome research

authors： Shen WJ,Patel S,Eriksson JE,Kraemer FB

更新日期：2010-04-05 00:00:00

abstract：:Snake venoms are biological weapon systems composed of secreted proteins and peptides that are used for immobilizing or killing prey. Although post-translational modifications are widely investigated because of their importance in many biological phenomena, we currently still have little understanding of how protein g...

journal_title：Journal of proteome research

authors： Andrade-Silva D,Zelanis A,Kitano ES,Junqueira-de-Azevedo IL,Reis MS,Lopes AS,Serrano SM

更新日期：2016-08-05 00:00:00

abstract：:A database of nonredundant structures of EF-hand domains--i.e., pairs of helix-loop-helix motifs--has been assembled, and the six angles among the four helices re-determined. A principal component analysis of these angles allows us to use two such components (PC1 and PC2) to describe the system retaining 80% of the to...

journal_title：Journal of proteome research

authors： Babini E,Bertini I,Capozzi F,Luchinat C,Quattrone A,Turano M

更新日期：2005-11-01 00:00:00

abstract：:We successfully modified an existing method to investigate protein-protein interactions in the pathogenic bacterium Salmonella enterica serovar Typhimurium (Salmonella Typhimurium). This method includes (i) addition of a histidine-biotin-histidine tag to the bait proteins via recombinant DNA techniques, (ii) in vivo c...

journal_title：Journal of proteome research

authors： Chowdhury SM,Shi L,Yoon H,Ansong C,Rommereim LM,Norbeck AD,Auberry KJ,Moore RJ,Adkins JN,Heffron F,Smith RD

更新日期：2009-03-01 00:00:00

abstract：:A synthetic coumarin, N,N,N-triethyl-11-(4-methyl-2-oxo-2H-chromen-7-yloxy)-11-oxoundecan-1-aminium bromide (SCD-1), having potent activity against pathogenic Aspergilli (MIC90 15.62 μg/mL), was investigated to identify its molecular targets in the pathogen. The proteome of Aspergillus fumigatus was developed after tr...

journal_title：Journal of proteome research

authors： Singh S,Gupta S,Singh B,Sharma SK,Gupta VK,Sharma GL

更新日期：2012-06-01 00:00:00