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Proteomic identification of in vivo interactors reveals novel function of skin cornification proteins.

Abstract：

:Protection against injurious external insults and loss of vital fluids is essential for life and is in all organisms, from bacteria to plants and humans, provided by some form of barrier. Members of the small proline-rich (SPRR) protein family are major components of the cornified cell envelope (CE), a structure responsible for the barrier properties of our skin. These proteins are efficient reactive oxygen species (ROS) quenchers involved not only in the establishment of the skin's barrier function but also in cell migration and wound healing. Here, a proteomic analysis of in vivo SPRR-interacting proteins confirmed their function in CE-formation and ROS-quenching and also revealed a novel unexpected role in DNA-binding. Direct in vitro and in vivo evidence proved that the DNA-binding capacity of SPRRs is regulated by the oxidation state of the proteins. At low ROS levels, nuclear SPRR is able to bind DNA and prevent ROS-induced DNA damage. When ROS levels increase, SPRR proteins multimerize and form an effective antioxidant barrier at the cell periphery, possibly to prevent the production or infiltration of ROS. At even higher ROS exposure, DNA-binding is restituted. A molecular model explaining how the intracellular oxidation state of SPRRs likely influences their selective protective function is provided.

journal_name

J Proteome Res

journal_title

Journal of proteome research

authors

Vermeij WP,Florea BI,Isenia S,Alia A,Brouwer J,Backendorf C

doi

10.1021/pr300310b

subject

Has Abstract

pub_date

2012-06-01 00:00:00

pages

3068-76

issue

6

eissn

1535-3893

issn

1535-3907

journal_volume

11

pub_type

杂志文章

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