Abstract：
:Histones are well-conserved proteins that form the basic structure of chromatin in eukaryotes and undergo several post-translational modifications, which are important for the control of transcription, replication, DNA damage repair, and chromosome condensation. In early branched organisms, histones are less conserved and appear to contain alternative sites for modifications, which could reveal evolutionary unique functions of histone modifications in gene expression and other chromatin-based processes. Here, by using high-resolution mass spectrometry, we identified and quantified histone post-translational modifications in two life cycle stages of Trypanosoma cruzi, the protozoan parasite that causes Chagas disease. We detected 44 new modifications, namely: 18 acetylations, seven monomethylations, seven dimethylations, seven trimethylations, and four phosphorylations. We found that replicative (epimastigote stage) contains more histone modifications than nonreplicative and infective parasites (trypomastigote stage). Acetylations of lysines at the C-terminus of histone H2A and methylations of lysine 23 of histone H3 were found to be enriched in trypomastigotes. In contrast, phosphorylation in serine 23 of H2B and methylations of lysine 76 of histone H3 predominates in proliferative states. The presence of one or two methylations in the lysine 76 was found in cells undergoing mitosis and cytokinesis, typical of proliferating parasites. Our findings provide new insights into the role of histone modifications related to the control of gene expression and cell-cycle regulation in an early divergent organism.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
de Jesus TC,Nunes VS,Lopes Mde C,Martil DE,Iwai LK,Moretti NS,Machado FC,de Lima-Stein ML,Thiemann OH,Elias MC,Janzen C,Schenkman S,da Cunha JPdoi
10.1021/acs.jproteome.6b00208subject
Has Abstractpub_date
2016-06-03 00:00:00pages
2039-51issue
6eissn
1535-3893issn
1535-3907journal_volume
15pub_type
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