Abstract:
:Our cyanylation (CN)-based methodology for determining disulfide structure of cystinyl proteins overcomes the limitations of conventional proteolytic methods. However, the CN-based method has the potential drawback that occasionally some CN-induced cleavage fragments may not be detected. We show that CN-based methods can overcome the failure to detect fragments by demonstrating the existence of small 'signature sets' of fragments. The link between signature sets and the robustness of CN-based methodology is validated by two case studies.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Wu W,Huang W,Qi J,Chou YT,Torng E,Watson JTdoi
10.1021/pr049961tkeywords:
subject
Has Abstractpub_date
2004-07-01 00:00:00pages
770-7issue
4eissn
1535-3893issn
1535-3907journal_volume
3pub_type
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