Abstract：
:Aquaporin (AQP) family plays a pivotal role in fluid secretion and absorption, especially in the digestive system and secretory glands. Within this family, AQP8 was reported to be widely expressed in the epithelia of the digestive tract, liver, and pancreas. In two parallel experimental platforms with different analytical and comparative approaches, in-gel tryptic digestion with macro-embedded spreadsheet analysis and in-solution tryptic digestion with LC-MS alignment based approach, we compared wild-type and AQP8 knockout mice colon proteomes. Shared result between both experiments revealed down-regulation of α-amylase 2 in AQP8-deleted mice model. Verification on both transcriptional and translational levels confirmed the involvement of AQP8 in α-amylase 2 regulation. Given the profound role of AQP8 as a water and solutes transporter, it might be important in modulating α-amylase 2 synthesis by colonic epithelial cells as well. Here, we also proved the capability of our coupled approaches for selecting the most reliable and significant candidates, an applicable process for initial screening of biological biomarkers in complex specimens and tissue extracts.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Magdeldin S,Li H,Yoshida Y,Satokata I,Maeda Y,Yokoyama M,Enany S,Zhang Y,Xu B,Fujinaka H,Yaoita E,Yamamoto Tdoi
10.1021/pr100789vsubject
Has Abstractpub_date
2010-12-03 00:00:00pages
6635-46issue
12eissn
1535-3893issn
1535-3907journal_volume
9pub_type
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