Characterization of Metabolomic Profile Associated with Metabolic Improvement after Bariatric Surgery in Subjects with Morbid Obesity.

abstract：:Nicotinamide adenine dinucleotide (NAD) is a coenzyme found in all living cells and mediates multiple cellular signaling pathways. In the present study, a 35% decrease of cellular NAD level is achieved by stable expression of the N-terminal truncated CD38, a NAD hydrolase. CD38-expressing (CD38(+)) cells have the lowe...

journal_title：Journal of proteome research

authors： Hu Y,Wang H,Wang Q,Deng H

更新日期：2014-02-07 00:00:00

abstract：:We have developed a novel approach to enhance phosphopeptide identification in liquid chromatography/mass spectrometry (LC/MS)-based phosphoproteomics. After enrichment of phosphopeptides with immobilized metal affinity chromatography (IMAC) and titanium dioxide (TiO(2)) microcolumns, samples were coinjected with ethy...

journal_title：Journal of proteome research

authors： Nakamura T,Myint KT,Oda Y

更新日期：2010-03-05 00:00:00

abstract：:The urine metabotype of 12 individuals was followed over a period of 8-10 years, which provided the longest longitudinal study of metabolic phenotypes to date. More than 2000 NMR metabolic profiles were analyzed. The majority of subjects have a stable metabotype. Subjects who were exposed to important pathophysiologic...

journal_title：Journal of proteome research

authors： Ghini V,Saccenti E,Tenori L,Assfalg M,Luchinat C

更新日期：2015-07-02 00:00:00

abstract：:The term "spectral clustering" is sometimes used to refer to the clustering of mass spectrometry data. However, it also classically refers to a family of popular clustering algorithms. To avoid confusion, a more specific term could advantageously be coined. ...

journal_title：Journal of proteome research

authors： Borges H,Guibert R,Permiakova O,Burger T

更新日期：2019-01-04 00:00:00

abstract：:Visual signal transduction takes place on the surface of flat membrane vesicles called photoreceptor discs, which reside inside the light-sensitive outer segment organelle of vertebrate photoreceptor cells. Although biochemical studies have indicated that discs are built with a handful of highly specialized proteins, ...

journal_title：Journal of proteome research

authors： Skiba NP,Spencer WJ,Salinas RY,Lieu EC,Thompson JW,Arshavsky VY

更新日期：2013-06-07 00:00:00

abstract：:Human saliva is a protein-rich, easily accessible source of potential local and systemic biomarkers to monitor changes that occur under pathological conditions; however, little is known about the changes in abundance associated with normal aging. In this study, we performed a comprehensive proteomic profiling of poole...

journal_title：Journal of proteome research

authors： Ambatipudi KS,Lu B,Hagen FK,Melvin JE,Yates JR

更新日期：2009-11-01 00:00:00

abstract：:Oxidative stress and inflammation play a role in the physiopathology of insulin resistance, diabetes and cardiovascular disease. A single high-fat, high-carbohydrate (HFHC) meal induces an increase in inflammatory and oxidative stress markers in peripheral blood mononuclear cells (PBMC). Previous studies have shown th...

journal_title：Journal of proteome research

authors： Chaves DFS,Carvalho PC,Brasili E,Rogero MM,Hassimotto NA,Diedrich JK,Moresco JJ,Yates JR 3rd,Lajolo FM

更新日期：2017-11-03 00:00:00

abstract：:Cytokinin hormones are crucial regulators of a large number of processes in plant development. Recently, significant progress has been made toward the elucidation of the molecular details of cytokinin that has led to a model for signal transduction involving a phosphorylation cascade. However, the current knowledge of...

journal_title：Journal of proteome research

authors： Heintz D,Erxleben A,High AA,Wurtz V,Reski R,Van Dorsselaer A,Sarnighausen E

更新日期：2006-09-01 00:00:00

abstract：:Proteomic studies were performed to identify the protein species involved in copper (Cu) stress responses in common wheat. Two-week-old wheat seedlings were exposed to 100 μM CuSO4 treatment for 3 days. Growth of shoots and roots was markedly inhibited and lipid peroxidation was greatly increased. Cu was readily absor...

journal_title：Journal of proteome research

authors： Li G,Peng X,Xuan H,Wei L,Yang Y,Guo T,Kang G

更新日期：2013-11-01 00:00:00

abstract：:After tissue or body fluid sampling, proteases and other protein-modifying enzymes can rapidly change composition of the proteome. As a direct consequence, analytical results will reflect a mix of in vivo proteome and ex vivo degradation products. Vital information about the presampling state may be destroyed or disto...

journal_title：Journal of proteome research

authors： Svensson M,Boren M,Sköld K,Fälth M,Sjögren B,Andersson M,Svenningsson P,Andren PE

更新日期：2009-02-01 00:00:00

abstract：:The epidermal growth factor receptor (EGFR) is usually overexpressed in nasopharyngeal carcinoma (NPC) and is associated with pathogenesis of NPC. However, while EGFR-modulated intracellular proteins have been extensively studied, little is known concerning their extracellular counterparts. To identify EGFR-regulated ...

journal_title：Journal of proteome research

authors： Tang CE,Guan YJ,Yi B,Li XH,Liang K,Zou HY,Yi H,Li MY,Zhang PF,Li C,Peng F,Chen ZC,Yao KT,Xiao ZQ

更新日期：2010-12-03 00:00:00

abstract：:Lung cancer, with its high metastatic potential and high mortality rate, is the worldwide leading cause of cancer-related deaths. High-throughput "omics"-based platforms have accelerated the discovery of biomarkers for lung cancer, and the resulting candidates are to be evaluated for their diagnostic potential as noni...

journal_title：Journal of proteome research

authors： Kim YJ,Sertamo K,Pierrard MA,Mesmin C,Kim SY,Schlesser M,Berchem G,Domon B

更新日期：2015-03-06 00:00:00

abstract：:Controlled ovarian hyperstimulation is performed to assist with generation of multiple mature oocytes for use in in vitro fertilization (IVF). The goal of our study was to evaluate differences in protein and steroid profiles in ovarian follicular fluid (hFF) samples obtained during oocyte retrieval from women undergoi...

journal_title：Journal of proteome research

authors： Kushnir MM,Naessén T,Wanggren K,Rockwood AL,Crockett DK,Bergquist J

更新日期：2012-10-05 00:00:00

abstract：:Salt stress is a major abiotic factor limiting maize yield. To characterize the mechanism underlying maize salt tolerance, we compared the seedling root proteomes of salt-tolerant Jing724 and salt-sensitive D9H. The germination rate and growth parameter values (weight and length) were higher for Jing724 than for D9H u...

journal_title：Journal of proteome research

authors： Luo M,Zhao Y,Wang Y,Shi Z,Zhang P,Zhang Y,Song W,Zhao J

更新日期：2018-01-05 00:00:00

abstract：:We developed an ELISA in high-density microarray format to detect hepatocyte growth factor (HGF) in human serum. The microassay can detect HGF at sub-pg/mL concentrations in sample volumes of 100 microL or less. The microassay is also quantitative and was used to detect elevated HGF levels in sera from recurrent breas...

journal_title：Journal of proteome research

authors： Woodbury RL,Varnum SM,Zangar RC

更新日期：2002-05-01 00:00:00

abstract：:Histones regulate a variety of chromatin templated events by their post-translational modifications (PTMs). Although there are extensive reports on the PTMs of canonical histones, the information on the histone variants remains very scanty. Here, we report the identification of different PTMs, such as acetylation, met...

journal_title：Journal of proteome research

authors： Pentakota SK,Sandhya S,P Sikarwar A,Chandra N,Satyanarayana Rao MR

更新日期：2014-12-05 00:00:00

abstract：:A selective method for the enrichment of O-GlcNAcylated peptides using a novel CLICK chemistry reagent is described. Peptides modified by O-GlcNAc were enzymatically labeled with N-azidoacetylgalactosamine. The azide was then reacted with a phospho-alkyne using CLICK chemistry and O-GlcNAcGalNAzPO(4)-containing peptid...

journal_title：Journal of proteome research

authors： Parker BL,Gupta P,Cordwell SJ,Larsen MR,Palmisano G

更新日期：2011-04-01 00:00:00

abstract：:Protein phosphorylation is the most abundant reversible covalent modification. Human protein kinases participate in almost all biological pathways, and approximately half of the kinases are associated with disease. PhoSigNet was designed to store and display human phosphorylation-mediated signal transduction networks,...

journal_title：Journal of proteome research

authors： Zhang M,Li H,He Y,Sun H,Xia L,Wang L,Sun B,Ma L,Zhang G,Li J,Li Y,Xie L

更新日期：2015-07-02 00:00:00

abstract：:We have investigated the transcriptome and proteome of the venom of a cryptic Australian elapid snake Drysdalia coronoides. To probe into the transcriptome, we constructed a partial cDNA library from the venom gland of D. coronoides. The proteome of the venom of D. coronoides was explored by tryptic digestion of the c...

journal_title：Journal of proteome research

authors： Chatrath ST,Chapeaurouge A,Lin Q,Lim TK,Dunstan N,Mirtschin P,Kumar PP,Kini RM

更新日期：2011-02-04 00:00:00

abstract：:This report describes the application of NMR spectroscopy to the profiling of metabolites in bronchoalveolar lavage fluid (BALf) of lung transplant recipients without bronchiolitis obliterans syndrome (BOS) (stable, S, n = 10), and with BOS at different degrees of severity (BOS 0p, n = 10; BOS I, n = 10). Through the ...

journal_title：Journal of proteome research

authors： Ciaramelli C,Fumagalli M,Viglio S,Bardoni AM,Piloni D,Meloni F,Iadarola P,Airoldi C

更新日期：2017-04-07 00:00:00

abstract：:We analyzed a unique rice (Oryza sativa L.) blast lesion mimic (blm) mutant for differentially expressed proteins in leaves of one- and two-week-old seedlings manifesting the lesion mimic phenotype. Gel-based one- and two-dimensional electrophoresis (1- and 2-DGE) was performed using leaves (blm and wild-type, WT) bef...

journal_title：Journal of proteome research

authors： Jung YH,Rakwal R,Agrawal GK,Shibato J,Kim JA,Lee MO,Choi PK,Jung SH,Kim SH,Koh HJ,Yonekura M,Iwahashi H,Jwa NS

更新日期：2006-10-01 00:00:00

abstract：:Chinese hamster ovary cells (CHO) are the most common cell line used in the production of therapeutic proteins. Understanding the complex pattern of secreted host cell proteins (HCP) that are released by CHO cells will facilitate the development of new recombinant protein production processes. In this study, we have a...

journal_title：Journal of proteome research

authors： Slade PG,Hajivandi M,Bartel CM,Gorfien SF

更新日期：2012-12-07 00:00:00

abstract：:As central tissue of glucose homeostasis, islet has been an important focus of diabetes research. Phosphorylation plays pivotal roles in islet function, especially in islet glucose-stimulated insulin secretion. A systematic view on how phosphorylation networks were coordinately regulated in this process remains lackin...

journal_title：Journal of proteome research

authors： Li J,Li Q,Tang J,Xia F,Wu J,Zeng R

更新日期：2015-11-06 00:00:00

abstract：:Protein chemical cross-linking combined with mass spectrometry has become an important technique for the analysis of protein structure and protein-protein interactions. A variety of cross-linkers are well developed, but reliable, rapid, and user-friendly tools for large-scale analysis of cross-linked proteins are stil...

journal_title：Journal of proteome research

authors： Lu L,Millikin RJ,Solntsev SK,Rolfs Z,Scalf M,Shortreed MR,Smith LM

更新日期：2018-07-06 00:00:00

abstract：:Large-scale metabolomics studies involving thousands of samples present multiple challenges in data analysis, particularly when an untargeted platform is used. Studies with multiple cohorts and analysis platforms exacerbate existing problems such as peak alignment and normalization. Therefore, there is a need for robu...

journal_title：Journal of proteome research

authors： Karaman I,Ferreira DL,Boulangé CL,Kaluarachchi MR,Herrington D,Dona AC,Castagné R,Moayyeri A,Lehne B,Loh M,de Vries PS,Dehghan A,Franco OH,Hofman A,Evangelou E,Tzoulaki I,Elliott P,Lindon JC,Ebbels TM

更新日期：2016-12-02 00:00:00

abstract：:Glycolysis is the core metabolic pathway supplying energy to cells. Whereas the vast majority of studies focus on specific aspects of the process, global analyses characterizing simultaneously all enzymes involved in the process are scarce. Here, we demonstrate that quantitative label- and standard-free proteomics all...

journal_title：Journal of proteome research

authors： Wiśniewski JR,Gizak A,Rakus D

更新日期：2015-08-07 00:00:00

abstract：:Analysis of the cerebrospinal fluid (CSF) proteome has proven valuable to the study of neurodegenerative disorders. To identify new protein/pathway alterations and candidate biomarkers for amyotrophic lateral sclerosis (ALS), we performed comparative proteomic profiling of CSF from sporadic ALS (sALS), healthy control...

journal_title：Journal of proteome research

authors： Collins MA,An J,Hood BL,Conrads TP,Bowser RP

更新日期：2015-11-06 00:00:00

abstract：:The Esx/WXG-100 (ESAT-6/Wss) exporters are multiprotein complexes that promote protein translocation across the cytoplasmic membrane in a diverse range of pathogenic and nonpathogenic bacterial species. The Esx-1 (ESAT-6 System-1) system mediates virulence factor translocation in mycobacterial pathogens, including the...

journal_title：Journal of proteome research

authors： Champion MM,Williams EA,Pinapati RS,Champion PA

更新日期：2014-11-07 00:00:00

abstract：:Hundreds of ribosomally synthesized cyclopeptides have been isolated from all domains of life, the vast majority having been reported in the last 15 years. Studies of cyclic peptides have highlighted their exceptional potential both as stable drug scaffolds and as biomedicines in their own right. Despite this, computa...

journal_title：Journal of proteome research

authors： Mohimani H,Liu WT,Mylne JS,Poth AG,Colgrave ML,Tran D,Selsted ME,Dorrestein PC,Pevzner PA

更新日期：2011-10-07 00:00:00

abstract：:Protein arrays will greatly accelerate research and development in medical and biological sciences. We have used cell-free protein biosynthesis and a parallel immobilization strategy for producing protein biochips. We demonstrate a model two-protein microarray using luciferase and green fluorescent protein, both expre...

journal_title：Journal of proteome research

authors： Oleinikov AV,Gray MD,Zhao J,Montgomery DD,Ghindilis AL,Dill K

更新日期：2003-05-01 00:00:00