Self-assembling protein arrays using electronic semiconductor microchips and in vitro translation.

abstract：:The role played by the degree of folding of protein backbones in explaining the binding energetics of protein-ligand interactions has been studied. We analyzed the protein/peptide interactions in the RNase-S system in which amino acids at two positions of the peptide S have been mutated. The global degree of folding o...

journal_title：Journal of proteome research

authors： Estrada E,Uriarte E,Vilar S

更新日期：2006-01-01 00:00:00

abstract：:We have obtained the first direct electrochemistry of full-length inducible nitric oxide synthase (iNOS) by entrapping the enzyme in polyethylenimine (PEI) film. The interaction between iNOS and calmodulin (CaM) was then studied, which revealed an enhanced electron-transfer reactivity of the enzyme facilitated by CaM....

journal_title：Journal of proteome research

authors： Xiao H,Zhou H,Chen G,Liu S,Li G

更新日期：2007-04-01 00:00:00

abstract：:To estimate the potential of the state-of-the-art proteomics technologies on full coverage of the encoding gene products, the Chinese Human Chromosome Proteome Consortium (CCPC) applied a multiomics strategy to systematically analyze the transciptome, translatome, and proteome of the same cultured hepatoma cells with ...

journal_title：Journal of proteome research

authors： Chang C,Li L,Zhang C,Wu S,Guo K,Zi J,Chen Z,Jiang J,Ma J,Yu Q,Fan F,Qin P,Han M,Su N,Chen T,Wang K,Zhai L,Zhang T,Ying W,Xu Z,Zhang Y,Liu Y,Liu X,Zhong F,Shen H,Wang Q,Hou G,Zhao H,Li G,Liu S,G

更新日期：2014-01-03 00:00:00

abstract：:Mass spectrometry (MS) has become an accessible tool for whole proteome quantitation with the ability to characterize protein expression across thousands of proteins within a single experiment. A subset of MS quantification methods (e.g., SILAC and label-free) monitor the relative intensity of intact peptides, where t...

journal_title：Journal of proteome research

authors： O'Brien JJ,O'Connell JD,Paulo JA,Thakurta S,Rose CM,Weekes MP,Huttlin EL,Gygi SP

更新日期：2018-01-05 00:00:00

abstract：:In-source fragmentation occurs as a byproduct of electrospray ionization. We find that ions produced as a result of in-source fragmentation often match fragment ions produced during MS/MS fragmentation, and we take advantage of this phenomenon in a novel algorithm to analyze LC-MS metabolomics data sets. Our approach ...

journal_title：Journal of proteome research

authors： Seitzer PM,Searle BC

更新日期：2019-02-01 00:00:00

abstract：:Oxidative stress and inflammation play a role in the physiopathology of insulin resistance, diabetes and cardiovascular disease. A single high-fat, high-carbohydrate (HFHC) meal induces an increase in inflammatory and oxidative stress markers in peripheral blood mononuclear cells (PBMC). Previous studies have shown th...

journal_title：Journal of proteome research

authors： Chaves DFS,Carvalho PC,Brasili E,Rogero MM,Hassimotto NA,Diedrich JK,Moresco JJ,Yates JR 3rd,Lajolo FM

更新日期：2017-11-03 00:00:00

abstract：:Increased serum haptoglobin concentration and changes in its glycosylation have been reported in certain cancer types. Information for hepatocellular carcinoma (HCC) has not yet been available. In this study, we aimed to carry out a systematic analysis of serum concentrations of haptoglobin (Hp) and its glycoforms in ...

journal_title：Journal of proteome research

authors： Ang IL,Poon TC,Lai PB,Chan AT,Ngai SM,Hui AY,Johnson PJ,Sung JJ

更新日期：2006-10-01 00:00:00

abstract：:Periodontal disease is perhaps the most common infectious disease in humans. Gingival crevicular fluid (GCF) is a local inflammatory exudate of the periodontal tissues. Its composition greatly varies between health and periodontal disease. GCF collection is rapid and noninvasive, but previous approaches aiming to anal...

journal_title：Journal of proteome research

authors： Bostanci N,Heywood W,Mills K,Parkar M,Nibali L,Donos N

更新日期：2010-05-07 00:00:00

abstract：:Starting in 2007, we have grown soybean (Glycine max [L.] Merr. variety Soniachna) and flax (Linum usitatissimum, L. variety Kyivskyi) in the radio-contaminated Chernobyl area and analyzed the seed proteomes. In the second-generation flax seeds, we detected a 12% increase in oil content. To characterize the bases for ...

journal_title：Journal of proteome research

authors： Klubicová K,Danchenko M,Skultety L,Berezhna VV,Rashydov NM,Hajduch M

更新日期：2013-11-01 00:00:00

abstract：:The missing human proteome comprises predicted protein-coding genes with no credible protein level evidence detected so far and constitutes ~18% of the human protein coding genes (neXtProt release 19/9/2014). The missing proteins may be of pharmacological interest as many of these are membrane receptors, thus requirin...

journal_title：Journal of proteome research

authors： Kumar D,Jain A,Dash D

更新日期：2015-12-04 00:00:00

abstract：:Our aim was to analyze the plasma proteome in aspirin (acetylsalicylic acid [ASA])-sensitive and ASA-resistant coronary ischemic patients. Plasma from 19 ASA-sensitive and 19 ASA-resistant patients was analyzed. For the proteomic study, two-dimensional electrophoresis was performed. The expression of one isotype of th...

journal_title：Journal of proteome research

authors： López-Farré AJ,Mateos-Cáceres PJ,Sacristán D,Azcona L,Bernardo E,de Prada TP,Alonso-Orgaz S,Fernández-Arquero M,Fernández-Ortiz A,Macaya C

更新日期：2007-07-01 00:00:00

abstract：:Protein citrullination (or deimination), an irreversible post-translational modification, has been implicated in several physiological and pathological processes, including gene expression regulation, apoptosis, rheumatoid arthritis, and Alzheimer's disease. Several research studies have been carried out on citrullina...

journal_title：Journal of proteome research

authors： Fert-Bober J,Venkatraman V,Hunter CL,Liu R,Crowgey EL,Pandey R,Holewinski RJ,Stotland A,Berman BP,Van Eyk JE

更新日期：2019-05-03 00:00:00

abstract：:Acinetobacter baumannii causes severe infections in compromised patients. We combined SDS-PAGE, two-dimensional gel electrophoresis and mass spectrometry (LC-MS/MS and MALDI-TOF) to separate and characterize the proteins of the cell envelope of this bacterium. In total, 135 proteins (inner and outer membrane proteins)...

journal_title：Journal of proteome research

authors： Siroy A,Cosette P,Seyer D,Lemaître-Guillier C,Vallenet D,Van Dorsselaer A,Boyer-Mariotte S,Jouenne T,Dé E

更新日期：2006-12-01 00:00:00

abstract：:Biomarkers for colorectal cancer (CRC) early diagnosis are currently lacking. The purpose of this study was to interpret molecular events in the early stage of CRC that may bring about new biomarkers for early diagnosis. Methylation isotope labeling assistant gel-enhanced liquid chromatography-mass spectrometry (GeLC-...

journal_title：Journal of proteome research

authors： Xie LQ,Zhao C,Cai SJ,Xu Y,Huang LY,Bian JS,Shen CP,Lu HJ,Yang PY

更新日期：2010-09-03 00:00:00

abstract：:Untargeted GC-TOF-MS analysis proved to be a suitable analytical platform to determine alterations in the metabolic profile. Several metabolic pathways were found to be altered in a first clinical study comparing smokers against nonsmokers. Subsequently, we conducted a clinical diet-controlled study to investigate alt...

journal_title：Journal of proteome research

authors： Goettel M,Niessner R,Mueller D,Scherer M,Scherer G,Pluym N

更新日期：2017-10-06 00:00:00

abstract：:Ethylene has been hypothesised to be a regulator of root nodule development in legumes, but its molecular mechanisms of action remain unclear. The skl mutant is an ethylene-insensitive legume mutant showing a hypernodulation phenotype when inoculated with its symbiont Sinorhizobium meliloti. We used the skl mutant to ...

journal_title：Journal of proteome research

authors： Prayitno J,Imin N,Rolfe BG,Mathesius U

更新日期：2006-11-01 00:00:00

abstract：:The solventogenic bacterium Clostridium acetobutylicum is an important species of the Clostridium community. To develop a fundamental tool that is useful for biological studies of C. acetobutylicum, we established a high resolution proteome reference map for this species. We identified 1206 spots representing 564 diff...

journal_title：Journal of proteome research

authors： Mao S,Luo Y,Zhang T,Li J,Bao G,Zhu Y,Chen Z,Zhang Y,Li Y,Ma Y

更新日期：2010-06-04 00:00:00

abstract：:Flooding has severe negative effects on soybean growth. To explore the flooding-responsive mechanisms in early-stage soybean, a phosphoproteomic approach was used. Two-day-old soybean plants were treated without or with flooding for 3, 6, 12, and 24 h, and root tip proteins were then extracted and analyzed at each tim...

journal_title：Journal of proteome research

authors： Yin X,Sakata K,Komatsu S

更新日期：2014-12-05 00:00:00

abstract：:The epidermal growth factor receptor (EGFR) is usually overexpressed in nasopharyngeal carcinoma (NPC) and is associated with pathogenesis of NPC. However, while EGFR-modulated intracellular proteins have been extensively studied, little is known concerning their extracellular counterparts. To identify EGFR-regulated ...

journal_title：Journal of proteome research

authors： Tang CE,Guan YJ,Yi B,Li XH,Liang K,Zou HY,Yi H,Li MY,Zhang PF,Li C,Peng F,Chen ZC,Yao KT,Xiao ZQ

更新日期：2010-12-03 00:00:00

abstract：:Campylobacter jejuni is the most common cause of bacterial gastroenteritis in the developed world. Immunoproteomics highlighted a 42-45 kDa antigen that comigrated on two-dimensional (2-DE) gels with the C. jejuni major outer membrane protein (MOMP). Predictive analysis revealed two candidates for the identity of the ...

journal_title：Journal of proteome research

authors： Scott NE,Bogema DR,Connolly AM,Falconer L,Djordjevic SP,Cordwell SJ

更新日期：2009-10-01 00:00:00

abstract：:The honeybees Apis mellifera ligustica (Aml) and Apis cerana cerana (Acc) are two different western and eastern bee species that evolved in distinct ecologies and developed specific antennal olfactory systems for their survival. Knowledge of how their antennal olfactory systems function in regards to the success of ea...

journal_title：Journal of proteome research

authors： Woltedji D,Song F,Zhang L,Gala A,Han B,Feng M,Fang Y,Li J

更新日期：2012-09-07 00:00:00

abstract：:As a recently validated reversible post translational modification, lysine malonylation regulates diverse cellular processes from bacteria to mammals, but its existence and function in photosynthetic organisms remain unknown. Cyanobacteria are the most ancient group of photosynthetic prokaryotes and contribute about 5...

journal_title：Journal of proteome research

authors： Ma Y,Yang M,Lin X,Liu X,Huang H,Ge F

更新日期：2017-05-05 00:00:00

abstract：:Mass spectrometry is the main analytical technique currently used to address the challenges of glycomics as it offers unrivalled levels of sensitivity and the ability to handle complex mixtures of different glycan variations. Determination of glycan structures from analysis of MS data is a major bottleneck in high-thr...

journal_title：Journal of proteome research

authors： Ceroni A,Maass K,Geyer H,Geyer R,Dell A,Haslam SM

更新日期：2008-04-01 00:00:00

abstract：:Clematis terniflora DC. has potential pharmaceutical value; on the contrary, high-level UV-B irradiation with dark treatment led to the accumulation of secondary metabolites. Metabolomic and proteomic analyses of leaf of C. terniflora were performed to investigate the systematic response mechanisms to high-level UV-B ...

journal_title：Journal of proteome research

authors： Yang B,Wang X,Gao C,Chen M,Guan Q,Tian J,Komatsu S

更新日期：2016-08-05 00:00:00

abstract：:Protein phosphatase 1 (PP1) catalytic subunits typically combine with other proteins that modulate their activity, direct them to distinct substrates, or serve as substrates for PP1. More than 50 PP1-interacting proteins (PIPs) have been identified so far. Given there are approximately 10 000 phosphoproteins in mammal...

journal_title：Journal of proteome research

authors： Flores-Delgado G,Liu CW,Sposto R,Berndt N

更新日期：2007-03-01 00:00:00

abstract：:Rheumatoid arthritis is a progressive, highly debilitating disease where early diagnosis, enabling rapid clinical intervention, would provide obvious benefits to patients, healthcare systems, and society. Novel biomarkers that enable noninvasive early diagnosis of the onset and progression of the disease provide one r...

journal_title：Journal of proteome research

authors： Michopoulos F,Karagianni N,Whalley NM,Firth MA,Nikolaou C,Wilson ID,Critchlow SE,Kollias G,Theodoridis GA

更新日期：2016-12-02 00:00:00

abstract：:Protein oxidation and nitration have been described during spinal cord injury (SCI) in animal models. Herein, mass spectrometry unambiguously identified GDP-dissociation inhibitor-2 (GDI-2) in SCI with post-translational modifications of 3-aminotyrosine (8 h post-injury) and an acrolein adduct of GDI-2 (72 h post-inju...

journal_title：Journal of proteome research

authors： John JP,Pintsov O,Petter-Puchner A,Redl H,Pollak A,Chen WQ,Lubec G

更新日期：2007-04-01 00:00:00

abstract：:Metabolically engineered Escherichia coli has previously been used to degrade cis-1,2-dichloroethylene (cis-DCE). The strains express the six genes of an evolved toluene ortho-monooxygenase from Burkholderia cepacia G4 (TOM-Green, which formed a reactive epoxide) with either (1) gamma-glutamylcysteine synthetase (GSHI...

journal_title：Journal of proteome research

authors： Lee J,Cao L,Ow SY,Barrios-Llerena ME,Chen W,Wood TK,Wright PC

更新日期：2006-06-01 00:00:00

abstract：:The process of extracting comprehensive proteome representations is a crucial step for many proteomic studies. While antibody microarrays are an evolving and promising methodology in proteomics, the issue of protein extraction from tissues for this kind of analysis has never been addressed. Here, we describe a single-...

journal_title：Journal of proteome research

authors： Alhamdani MS,Schröder C,Werner J,Giese N,Bauer A,Hoheisel JD

更新日期：2010-02-05 00:00:00

abstract：:One of the critical challenges in predicting protein subcellular localization is how to deal with the case of multiple location sites. Unfortunately, so far, no efforts have been made in this regard except for the one focused on the proteins in budding yeast only. For most existing predictors, the multiple-site protei...

journal_title：Journal of proteome research

authors： Chou KC,Shen HB

更新日期：2007-05-01 00:00:00