Abstract：
:Clematis terniflora DC. has potential pharmaceutical value; on the contrary, high-level UV-B irradiation with dark treatment led to the accumulation of secondary metabolites. Metabolomic and proteomic analyses of leaf of C. terniflora were performed to investigate the systematic response mechanisms to high-level UV-B irradiation with dark treatment. Metabolites related to carbohydrates, fatty acids, and amino acids and/or proteins related to stress, cell wall, and amino acid metabolism were gradually increased in response to high-level UV-B irradiation with dark treatment. On the basis of cluster analysis and mapping of proteins related to amino acid metabolism, the abundances of S-adenosylmethionine synthetase and cysteine synthase as well as 1,1-diphenyl-2-picrylhydrazyl scavenging activity were gradually increased in response to high-level UV-B irradiation with dark treatment. Furthermore, the abundance of dihydrolipoyl dehydrogenase/glutamate dehydrogenase and the content of γ-aminobutyric acid were also increased following high-level UV-B irradiation with dark treatment. Taken together, these results suggest that high-level UV-B irradiation with dark treatment induces the activation of reactive oxygen species scavenging system and γ-aminobutyric acid shunt pathway in leaf of C. terniflora.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Yang B,Wang X,Gao C,Chen M,Guan Q,Tian J,Komatsu Sdoi
10.1021/acs.jproteome.6b00206subject
Has Abstractpub_date
2016-08-05 00:00:00pages
2643-57issue
8eissn
1535-3893issn
1535-3907journal_volume
15pub_type
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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更新日期：2007-09-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2008-01-01 00:00:00
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journal_title：Journal of proteome research
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更新日期：2019-12-06 00:00:00
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更新日期：2006-09-01 00:00:00
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journal_title：Journal of proteome research
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更新日期：2020-10-20 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr8001723
更新日期：2008-11-01 00:00:00
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