Abstract:
:Cystinuria is a purely renal, rare genetic disease caused by mutations in cystine transporter genes and characterized by defective cystine reabsorption leading to kidney stones. In 14% of cases, patients undergo nephrectomy, but given the difficulty to predict the evolution of the disease, the identification of markers of kidney damage would improve the follow-up of patients with a higher risk. The aim of the present study is to develop a robust, reproducible, and noninvasive methodology for proteomic analysis of urinary exosomes using high resolution mass spectrometry. A clinical pilot study conducted on eight cystinuria patients versus 10 controls highlighted 165 proteins, of which 38 were up-regulated, that separate cystinuria patients from controls and further discriminate between severe and moderate forms of the disease. These proteins include markers of kidney injury, circulating proteins, and a neutrophil signature. Analysis of selected proteins by immunobloting, performed on six additional cystinuria patients, validated the mass spectrometry data. To our knowledge, this is the first successful proteomic study in cystinuria unmasking the potential role of inflammation in this disease. The workflow we have developed is applicable to investigate urinary exosomes in different renal diseases and to search for diagnostic/prognostic markers. Data are available via ProteomeXchange with identifier PXD001430.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Bourderioux M,Nguyen-Khoa T,Chhuon C,Jeanson L,Tondelier D,Walczak M,Ollero M,Bekri S,Knebelmann B,Escudier E,Escudier B,Edelman A,Guerrera ICdoi
10.1021/pr501003qsubject
Has Abstractpub_date
2015-01-02 00:00:00pages
567-77issue
1eissn
1535-3893issn
1535-3907journal_volume
14pub_type
杂志文章abstract::Tomato fruit and seed allergens were detected by IgE-immunoblotting using sera from 18 adult tomato-sensitized patients selected based on a positive history skin prick test (SPT) and specific Immunglobulin (Ig) E-levels. Isolated tomato seed total protein showed high SPT activity comparable or even higher than tomato ...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr800186d
更新日期:2009-03-01 00:00:00
abstract::Cannabinoid 2 receptor (CB2R), a Class-A G-protein coupled receptor (GPCR), is a promising drug target under a wide array of pathological conditions. Rational drug design has been hindered due to our poor understanding of the structural features involved in ligand binding. Binding of a high-affinity biarylpyrazole inv...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.7b00023
更新日期:2017-07-07 00:00:00
abstract::Gastric cancer (GC) is among the most common cancers worldwide. Gastric carcinogenesis is a multistep and multifactorial process beginning with chronic gastritis induced by Helicobacter pylori (H. pylori) infection. This process is often described via a sequence of events known as Correas's cascade, a stepwise progres...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.6b00281
更新日期:2016-08-05 00:00:00
abstract::Histone post-translational modifications (PTMs) are fundamental players of chromatin regulation, as they contribute to editing histone chemical properties and recruiting proteins for gene transcription and DNA repair. Mass spectrometry (MS)-based proteomics is currently the most widely adopted strategy for high-throug...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.7b00544
更新日期:2018-01-05 00:00:00
abstract::In shotgun proteomics analysis, user-specified parameters are critical to database search performance and therefore to the yield of confident peptide-spectrum matches (PSMs). Two of the most important parameters are related to the accuracy of the mass spectrometer. Precursor mass tolerance defines the peptide candidat...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.7b00028
更新日期:2017-04-07 00:00:00
abstract::Selective capture of glycopolypeptides followed by release and analysis of the former glycosylation-site peptides has been shown to have promise for reducing the complexity of body fluids such as blood for biomarker discovery. In this work, a protocol based on capture of polypeptides containing a N-linked carbohydrate...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr900845m
更新日期:2010-04-05 00:00:00
abstract::Periodontal disease is perhaps the most common infectious disease in humans. Gingival crevicular fluid (GCF) is a local inflammatory exudate of the periodontal tissues. Its composition greatly varies between health and periodontal disease. GCF collection is rapid and noninvasive, but previous approaches aiming to anal...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr900941z
更新日期:2010-05-07 00:00:00
abstract::Cyadox is an antibiotic drug and has the potential to be used as a feedstuff additive in promoting the growth of animals. However, the toxicity of cyadox should be fully assessed before application, and this has prompted the current investigation on the metabolic responses of mice to cyadox exposure, using a metabonom...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr301033a
更新日期:2013-01-04 00:00:00
abstract::Microbes are known to regulate both gene expression and protein activity through the use of post-translational modifications (PTMs). Common PTMs involved in cellular signaling and gene control include methylations, acetylations, and phosphorylations, whereas oxidations have been implicated as an indicator of stress. S...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr070531n
更新日期:2008-02-01 00:00:00
abstract::Expansion of human articular chondrocytes (HACs) in serum-free medium (SFM) has been shown to be enhanced by the control of medium osmolality. HACs attained better growth when cultured at osmolalities lower than the average osmolality found in articular cartilage in vivo, although the specific causes for this improved...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr100136q
更新日期:2010-05-07 00:00:00
abstract::The solventogenic bacterium Clostridium acetobutylicum is an important species of the Clostridium community. To develop a fundamental tool that is useful for biological studies of C. acetobutylicum, we established a high resolution proteome reference map for this species. We identified 1206 spots representing 564 diff...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr9012078
更新日期:2010-06-04 00:00:00
abstract::The last two decades have seen a broad diversity of methods used to identify and/or characterize proteins in the archeological and paleontological record. Of these, mass spectrometry has opened an unprecedented window into the proteomes of the past, providing protein sequence data from long extinct animals as well as ...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.7b00703
更新日期:2018-03-02 00:00:00
abstract::One of the critical challenges in predicting protein subcellular localization is how to deal with the case of multiple location sites. Unfortunately, so far, no efforts have been made in this regard except for the one focused on the proteins in budding yeast only. For most existing predictors, the multiple-site protei...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr060635i
更新日期:2007-05-01 00:00:00
abstract::One of the ultimate goals of systems biology research is to obtain a comprehensive understanding of the control mechanisms of complex cellular metabolisms. Metabolic Flux Analysis (MFA) is a important method for the quantitative estimation of intracellular metabolic flows through metabolic pathways and the elucidation...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr2002885
更新日期:2011-08-05 00:00:00
abstract::Protein phosphatase 1 (PP1) catalytic subunits typically combine with other proteins that modulate their activity, direct them to distinct substrates, or serve as substrates for PP1. More than 50 PP1-interacting proteins (PIPs) have been identified so far. Given there are approximately 10 000 phosphoproteins in mammal...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr060504h
更新日期:2007-03-01 00:00:00
abstract::As a model carcinogen in studying the mutagenicity and carcinogenicity of N-nitroso alkylating agent, N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) can induce DNA damages and many cellular defensive events, such as DNA repair, G2/M-phase arrest and apoptosis. Noticeably, diverse cellular responses were observed to occur...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr900008n
更新日期:2009-06-01 00:00:00
abstract::Novel biomarker verification assays are urgently required to improve the efficiency of biomarker development. Benefitting from lower development costs, multiple reaction monitoring (MRM) has been used for biomarker verification as an alternative to immunoassay. However, in general MRM analysis, only one sample can be ...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr4005025
更新日期:2013-09-06 00:00:00
abstract::Growing appreciation for astrocytes as active participants in nervous system development, neurovascular metabolic coupling, and neurological disease progression has stimulated recent investigation into specific astrocyte-secreted proteins that may mediate these functions. The current work utilized SILAC-generated isot...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr100134n
更新日期:2010-05-07 00:00:00
abstract::Energy imbalance is a primary cause of obesity. While the classical approach to attenuate weight gain includes an increase in energy expenditure through exercise, dietary manipulation such as the inclusion of dairy products has also been proven effective. In the present study, we explored the potential mechanisms by w...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.9b00304
更新日期:2019-11-01 00:00:00
abstract::The current technique used for microbial identification in hospitals is matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). However, it suffers from important limitations, in particular, for closely related species or when the database used for the identification lacks the appr...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.0c00351
更新日期:2021-01-01 00:00:00
abstract::Knowledge of protein subcellular localization assists in the elucidation of protein function and understanding of different biological mechanisms that occur at discrete subcellular niches. Organelle-centric proteomics enables localization of thousands of proteins simultaneously. Although such techniques have successfu...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr4008464
更新日期:2014-02-07 00:00:00
abstract::Prediction of the types of membrane proteins is of great importance both for genome-wide annotation and for experimental researchers to understand proteins' functions. We describe a new strategy for the prediction of the types of membrane proteins using the Nearest Neighbor Algorithm. We introduced a bipartite feature...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr700715c
更新日期:2008-03-01 00:00:00
abstract::The Model Organism Protein Expression Database (MOPED, http://moped.proteinspire.org) is an expanding proteomics resource to enable biological and biomedical discoveries. MOPED aggregates simple, standardized and consistently processed summaries of protein expression and metadata from proteomics (mass spectrometry) ex...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr400884c
更新日期:2014-01-03 00:00:00
abstract::Clostridium difficile in recent years has undergone rapid evolution and has emerged as a serious human pathogen. Proteomic approaches can improve the understanding of the diversity of this important pathogen, especially in comparing the adaptive ability of different C. difficile strains. In this study, TMT labeling an...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr3007528
更新日期:2013-03-01 00:00:00
abstract::Imatinib is the first molecular targeted therapy that has shown clinical success, but imatinib acquired resistance, although a rare event, is critical during the therapy of chronic myelogenous leukaemia (CML). With the aim of better understanding the molecular mechanisms accompanying acquisition of resistance to this ...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr0603708
更新日期:2007-01-01 00:00:00
abstract::Formalin-fixed, paraffin-embedded (FFPE) tissues have been used to discover disease-associated protein changes using mass spectrometry. Protein post-translational modifications such as glycosylation are known to associate with disease development. In this study, we investigated whether FFPE tissues preserve such modif...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr800952h
更新日期:2009-04-01 00:00:00
abstract::Activation of AMP-activated protein kinase (AMPK) upon energy depletion stimulates energy production and limits energy utilization. Erythrocytes lacking AMPK are susceptible to suicidal cell death (eryptosis). A hallmark of eryptosis is cell membrane scrambling with phosphatidylserine exposure at the erythrocyte surfa...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr101004j
更新日期:2011-04-01 00:00:00
abstract::Reversible phosphorylations play a critical role in most biological pathways. Hence, in signaling studies great effort has been put into identification of a maximum number of phosphosites per experiment. Mass spectrometry (MS)-based phosphoproteomics approaches have been proven to be an ideal analytical method for map...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr200092z
更新日期:2011-08-05 00:00:00
abstract::The exact impact of bariatric surgery in metabolically "healthy" (MH) or "unhealthy" (MU) phenotypes for the study of the metabolic improvement is still unknown. We applied an untargeted LC-ESI-TripleTOF-MS-driven metabolomics approach in serum samples from 39 patients with morbid obesity (MH and MU) 1, 3, and 6 month...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.8b00144
更新日期:2018-08-03 00:00:00
abstract::A biphasic monolithic capillary column with 10 cm segment of strong-cation exchange monolith and 65 cm segment of reversed-phase monolith was prepared within a single 100 microm i.d. capillary. Separation performance of this column was evaluated by a five-cycle online multidimensional separation of 10 microg tryptic d...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr700562b
更新日期:2008-01-01 00:00:00