Abstract：
:During vertebrate central nervous system development, the apical neuroepithelium is bathed with embryonic Cerebrospinal Fluid (e-CSF) which plays regulatory roles in cortical cell proliferation and maintenance. Here, we report the first proteomic analysis of human e-CSF and compare it to an extensive proteomic analysis of rat e-CSF. As expected, we identified a large collection of protease inhibitors, extracellular matrix proteins, and transport proteins in CSF. However, we also found a surprising suite of signaling and intracellular proteins not predicted by previous proteomic analysis. Some of the intracellular proteins are likely to represent the contents of microvesicles recently described within the CSF (Marzesco, A. M., et al. J. Cell Sci. 2005, 118 (Pt. 13), 2849-2858). Defining the rich composition of e-CSF will enable a greater understanding of its concerted actions during critical stages of brain development.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Zappaterra MD,Lisgo SN,Lindsay S,Gygi SP,Walsh CA,Ballif BAdoi
10.1021/pr070247wsubject
Has Abstractpub_date
2007-09-01 00:00:00pages
3537-48issue
9eissn
1535-3893issn
1535-3907journal_volume
6pub_type
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