Abstract：
:Liquid chromatography in coupling with high-resolution ESI-LTQ-Orbitrap mass spectrometry was applied for a proteomic study of pediatric pilocytic astrocytoma brain tumor intracystic fluid by an integrated top-down/bottom-up platform. Both of the proteomic strategies resulted complementary and support each other in contributing to a wide characterization of the protein and peptide content of the tumor fluid. Top-down approach allowed to identify several proteins and peptides involved in different biological activities together with the characterization of interesting proteoforms such as fibrinopeptide A and its truncated form, fibrinopeptide B, complement C3f fragments, β-thymosin peptides, ubiquitin, several apolipoproteins belonging to A and C families, apolipoprotein J and D, and cystatin C. Of particular interest resulted the identification of a N-terminal truncated cystatin C proteoform, likely involved in immune response mechanism modulations and the identification of oxidized and glycosylated apolipoproteins including disulfide bridge dimeric forms. The bottom-up approach confirmed some of the experimental data findings together with adding the characterization of high-molecular-mass proteins in the samples. These data could contribute to elucidate the molecular mechanisms involved in onset and progression of the disease and cyst development.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Inserra I,Iavarone F,Martelli C,D'Angelo L,Delfino D,Rossetti DV,Tamburrini G,Massimi L,Caldarelli M,Di Rocco C,Messana I,Castagnola M,Desiderio Cdoi
10.1021/pr500806ksubject
Has Abstractpub_date
2014-11-07 00:00:00pages
4594-606issue
11eissn
1535-3893issn
1535-3907journal_volume
13pub_type
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journal_title：Journal of proteome research
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