Abstract：
:Using an integrated transcriptomic and proteomic approach, we characterized the venom peptidome of the European red ant, Manica rubida. We identified 13 "myrmicitoxins" that share sequence similarities with previously identified ant venom peptides, one of them being identified as an EGF-like toxin likely resulting from a threonine residue modified by O-fucosylation. Furthermore, we conducted insecticidal assays of reversed-phase HPLC venom fractions on the blowfly Lucilia caesar, permitting us to identify six myrmicitoxins (i.e., U3-, U10-, U13-, U20-MYRTX-Mri1a, U10-MYRTX-Mri1b, and U10-MYRTX-Mri1c) with an insecticidal activity. Chemically synthesized U10-MYRTX-Mri1a, -Mri1b, -Mri1c, and U20-MYRTX-Mri1a irreversibly paralyzed blowflies at the highest doses tested (30-125 nmol·g-1). U13-MYRTX-Mri1a, the most potent neurotoxic peptide at 1 h, had reversible effects after 24 h (150 nmol·g-1). Finally, U3-MYRTX-Mri1a has no insecticidal activity, even at up to 55 nmol·g-1. Thus, M. rubida employs a paralytic venom rich in linear insecticidal peptides, which likely act by disrupting cell membranes.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Touchard A,Aili SR,Téné N,Barassé V,Klopp C,Dejean A,Kini RM,Mrinalini,Coquet L,Jouenne T,Lefranc B,Leprince J,Escoubas P,Nicholson GM,Treilhou M,Bonnafé Edoi
10.1021/acs.jproteome.0c00048subject
Has Abstractpub_date
2020-04-03 00:00:00pages
1800-1811issue
4eissn
1535-3893issn
1535-3907journal_volume
19pub_type
杂志文章abstract：:Colorectal cancer (CRC) is the third most common cancer worldwide and has poor prognosis. To identify the proteins involved in colorectal carcinogenesis, we employed 2-DE and MALDI-TOF/TOF-based proteomics approach to study the differentially expressed proteins in tumor and adjacent nontumor tissue samples. Samples fr...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr900365e
更新日期：2009-10-01 00:00:00
abstract：:Protein cleavage coupled with isotope dilution mass spectrometry (PC-IDMS) has the potential to provide the absolute concentration of a specific protein, or multiple proteins, in complex mixtures. However, PC-IDMS differs from standard IDMS since the internal standard is a different molecule than the analyte at the st...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr034124x
更新日期：2004-05-01 00:00:00
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journal_title：Journal of proteome research
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doi：10.1021/pr050298y
更新日期：2006-02-01 00:00:00
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journal_title：Journal of proteome research
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doi：10.1021/pr0503174
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr4006712
更新日期：2013-12-06 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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journal_title：Journal of proteome research
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更新日期：2010-04-05 00:00:00
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr8006446
更新日期：2009-02-01 00:00:00
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journal_title：Journal of proteome research
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更新日期：2013-05-03 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr7008109
更新日期：2008-04-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr500409q
更新日期：2014-07-03 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.9b00615
更新日期：2020-04-03 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr100136q
更新日期：2010-05-07 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr3007528
更新日期：2013-03-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr700800s
更新日期：2008-03-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr500202e
更新日期：2014-08-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2013-02-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2017-02-03 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2014-11-07 00:00:00
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2015-07-02 00:00:00
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journal_title：Journal of proteome research
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更新日期：2016-01-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2008-11-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2020-04-03 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2008-02-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2020-11-06 00:00:00
abstract：:A selective method for the enrichment of O-GlcNAcylated peptides using a novel CLICK chemistry reagent is described. Peptides modified by O-GlcNAc were enzymatically labeled with N-azidoacetylgalactosamine. The azide was then reacted with a phospho-alkyne using CLICK chemistry and O-GlcNAcGalNAzPO(4)-containing peptid...
journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2011-04-01 00:00:00
