Two-dimensional liquid chromatography-tandem mass spectrometry coupled with isobaric tags for relative and absolute quantification (iTRAQ) labeling approach revealed first proteome profiles of pulmonary alveolar macrophages infected with porcine reproduct

abstract：:Pathogenic events in Alzheimer's disease are believed to involve an imbalance between the production and clearance of the neurotoxic 42 amino acid form of the beta-amyloid peptide (Abeta1-42). Although much is known about the production of Abeta1-42, many questions remain about its degradation. Here, we describe an op...

journal_title：Journal of proteome research

authors： Portelius E,Tran AJ,Andreasson U,Persson R,Brinkmalm G,Zetterberg H,Blennow K,Westman-Brinkmalm A

更新日期：2007-11-01 00:00:00

abstract：:We have investigated whether postexercise ingestion of carbohydrates in combination with proteins generates a different systemic metabolic response, as compared to the sole ingestion of carbohydrate or water, in the early recovery phase following exercise. In addition, metabolic patterns related to fitness level were ...

journal_title：Journal of proteome research

authors： Chorell E,Moritz T,Branth S,Antti H,Svensson MB

更新日期：2009-06-01 00:00:00

abstract：:FDA oversight of medical devices, including in vitro diagnostic devices (IVDs or laboratory tests), in the United States was a direct result of the passage of the Medical Device Amendments of 1976. This law introduced a series of general controls for medical devices including registration and listing, requirements for...

journal_title：Journal of proteome research

authors： Hackett JL,Gutman SI

更新日期：2005-07-01 00:00:00

abstract：:The term "spectral clustering" is sometimes used to refer to the clustering of mass spectrometry data. However, it also classically refers to a family of popular clustering algorithms. To avoid confusion, a more specific term could advantageously be coined. ...

journal_title：Journal of proteome research

authors： Borges H,Guibert R,Permiakova O,Burger T

更新日期：2019-01-04 00:00:00

abstract：:Lipid rafts are dynamic membrane microdomains that orchestrate molecular interactions and are implicated in cancer development. To understand the functions of lipid rafts in cancer, we performed an integrated analysis of quantitative lipid raft proteomics data sets modeling progression in breast cancer, melanoma, and ...

journal_title：Journal of proteome research

authors： Shah AD,Inder KL,Shah AK,Cristino AS,McKie AB,Gabra H,Davis MJ,Hill MM

更新日期：2016-10-07 00:00:00

abstract：:Natural killer (NK) cells are important components of innate immune defense. NK cells kill virus-infected cells and secrete cytokines that are involved in activation of other immune cells. Macrophage-derived cytokines interferon-alpha (IFN-alpha) and interleukin-15 (IL-15) are in turn important activators of NK cells,...

journal_title：Journal of proteome research

authors： Rakkola R,Matikainen S,Nyman TA

更新日期：2005-01-01 00:00:00

abstract：:Acinetobacter baumannii causes severe infections in compromised patients. We combined SDS-PAGE, two-dimensional gel electrophoresis and mass spectrometry (LC-MS/MS and MALDI-TOF) to separate and characterize the proteins of the cell envelope of this bacterium. In total, 135 proteins (inner and outer membrane proteins)...

journal_title：Journal of proteome research

authors： Siroy A,Cosette P,Seyer D,Lemaître-Guillier C,Vallenet D,Van Dorsselaer A,Boyer-Mariotte S,Jouenne T,Dé E

更新日期：2006-12-01 00:00:00

abstract：:Difference gel electrophoresis (DiGE) allows the reliable comparison of proteome differences between two or three samples within a single gel, by way of a CyDye fluorescent labeling system. This facilitates identification of protein differences avoiding the difficulties associated with gel-to-gel variation. A drawback...

journal_title：Journal of proteome research

authors： Hannigan A,Burchmore R,Wilson JB

更新日期：2007-09-01 00:00:00

abstract：:Elevated atmospheric CO2 and nitrogen are major environmental factors affecting shoot growth. The objectives of this study are to determine the interactive effects of elevated CO2 and nitrogen on leaf growth in tall fescue ( Festuca arundinacea) and to identify major proteins and associated metabolic pathways underlyi...

journal_title：Journal of proteome research

authors： Yu J,Fan N,Li R,Zhuang L,Xu Q,Huang B

更新日期：2019-06-07 00:00:00

abstract：:By foliar fortification with selenite, selenium (Se)-enriched rice with a higher Se content and grain yield has been generated. However, the regulatory mechanisms of Se response in rice grains remain unknown; therefore, we carried out a comparative proteomics study in Se-enriched rice grains by using two approaches in...

journal_title：Journal of proteome research

authors： Wang YD,Wang X,Ngai SM,Wong YS

更新日期：2013-02-01 00:00:00

abstract：:The current technique used for microbial identification in hospitals is matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). However, it suffers from important limitations, in particular, for closely related species or when the database used for the identification lacks the appr...

journal_title：Journal of proteome research

authors： Dupré M,Duchateau M,Malosse C,Borges-Lima D,Calvaresi V,Podglajen I,Clermont D,Rey M,Chamot-Rooke J

更新日期：2021-01-01 00:00:00

abstract：:Royal jelly (RJ) is an exclusive food for queen honey bee (Apis mellifera L.) that is synthesized and secreted by young worker bees. RJ is also widely used in medical products, cosmetics, and as health foods. However, little is known about RJ functionality and the total protein components, although recent research is ...

journal_title：Journal of proteome research

authors： Furusawa T,Rakwal R,Nam HW,Shibato J,Agrawal GK,Kim YS,Ogawa Y,Yoshida Y,Kouzuma Y,Masuo Y,Yonekura M

更新日期：2008-08-01 00:00:00

abstract：:Hydrogen peroxide (H(2)O(2)) and nitric oxide ((•)NO) elicit numerous processes in plants. However, our knowledge of H(2)O(2) and (•)NO-responsive proteins is limited. The present study aimed to identify proteins whose accumulation levels were regulated by these signaling molecules in citrus leaves. To address this qu...

journal_title：Journal of proteome research

authors： Tanou G,Job C,Belghazi M,Molassiotis A,Diamantidis G,Job D

更新日期：2010-11-05 00:00:00

abstract：:The secreted proteins of bacteria are usually accompanied by virulence factors, which can cause inflammation and damage host cells. Identifying the secretomes arising from the interactions of bacteria and host cells could therefore increase understanding of the mechanisms during initial pathogenesis. The present study...

journal_title：Journal of proteome research

authors： Chiu KH,Wang LH,Tsai TT,Lei HY,Liao PC

更新日期：2017-01-06 00:00:00

abstract：:Protein phosphorylation is a major mechanism of signal transduction in bacteria. Here, we analyzed the proteome and phosphoproteome of a wild-type strain of the food-borne pathogen Listeria monocytogenes that was grown in either chemically defined medium or rich medium containing glucose. We then compared these result...

journal_title：Journal of proteome research

authors： Misra SK,Moussan Désirée Aké F,Wu Z,Milohanic E,Cao TN,Cossart P,Deutscher J,Monnet V,Archambaud C,Henry C

更新日期：2014-12-05 00:00:00

abstract：:During asexual intraerythrocytic development, Plasmodium falciparum diverges from the paradigm of the eukaryotic cell cycles by undergoing multiple rounds of DNA replication and nuclear division without cytokinesis. A better understanding of the molecular switches that coordinate a myriad of events for the progression...

journal_title：Journal of proteome research

authors： Pease BN,Huttlin EL,Jedrychowski MP,Talevich E,Harmon J,Dillman T,Kannan N,Doerig C,Chakrabarti R,Gygi SP,Chakrabarti D

更新日期：2013-09-06 00:00:00

abstract：:The ability to distinguish between phosphopeptides of high and low stoichiometry is essential to discover the true extent of protein phosphorylation. We here extend the strategy whereby a peptide sample is briefly split in two identical parts and differentially labeled preceding the phosphatase treatment of one part. ...

journal_title：Journal of proteome research

authors： Glibert P,Meert P,Van Steendam K,Van Nieuwerburgh F,De Coninck D,Martens L,Dhaenens M,Deforce D

更新日期：2015-02-06 00:00:00

abstract：:Francisella tularensis is a Gram-negative bacterium that causes the zoonotic disease tularemia. The historical development of tularemia as a biological weapon has led to it being characterized by the CDC as a category A biothreat agent. Neither posttranslational modification (PTM) of proteins, in particular lysine ace...

journal_title：Journal of proteome research

authors： Marakasova E,Ii A,Nelson KT,van Hoek ML

更新日期：2020-04-03 00:00:00

abstract：:A proper sample preparation, in particular, abundant protein removal is crucial in the characterization of low-abundance proteins including those harboring post-translational modifications. In human cerebrospinal fluid (CSF), approximately 80% of proteins originate from serum, and removal of major proteins is necessar...

journal_title：Journal of proteome research

authors： Ogata Y,Charlesworth MC,Muddiman DC

更新日期：2005-05-01 00:00:00

abstract：:Cyadox is an antibiotic drug and has the potential to be used as a feedstuff additive in promoting the growth of animals. However, the toxicity of cyadox should be fully assessed before application, and this has prompted the current investigation on the metabolic responses of mice to cyadox exposure, using a metabonom...

journal_title：Journal of proteome research

authors： Huang C,Lei H,Zhao X,Tang H,Wang Y

更新日期：2013-01-04 00:00:00

abstract：:Facing the explosion of newly generated protein sequences in the post genomic era, we are challenged to develop an automated method for fast and reliably annotating their subcellular locations. Knowledge of subcellular locations of proteins can provide useful hints for revealing their functions and understanding how t...

journal_title：Journal of proteome research

authors： Chou KC,Shen HB

更新日期：2006-08-01 00:00:00

abstract：:Liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based proteomic methods have been widely used to identify lysine acylation proteins. However, these experimental approaches often fail to detect proteins that are in low abundance or absent in specific biological samples. To circumvent these problems, we develo...

journal_title：Journal of proteome research

authors： Du Y,Zhai Z,Li Y,Lu M,Cai T,Zhou B,Huang L,Wei T,Li T

更新日期：2016-12-02 00:00:00

abstract：:Dysregulated protein phosphorylation is a primary culprit in multiple physiopathological states. Hence, although analysis of signaling cascades on a proteome-wide scale would provide significant insight into both normal and aberrant cellular function, such studies are simultaneously limited by sheer biological complex...

journal_title：Journal of proteome research

authors： Ndassa YM,Orsi C,Marto JA,Chen S,Ross MM

更新日期：2006-10-01 00:00:00

abstract：:The enormous challenges of mass spectrometry-based metaproteomics are primarily related to the analysis and interpretation of the acquired data. This includes reliable identification of mass spectra and the meaningful integration of taxonomic and functional meta-information from samples containing hundreds of unknown ...

journal_title：Journal of proteome research

authors： Muth T,Behne A,Heyer R,Kohrs F,Benndorf D,Hoffmann M,Lehtevä M,Reichl U,Martens L,Rapp E

更新日期：2015-03-06 00:00:00

abstract：:Comparative phosphoproteomics of Mycobacterium tuberculosis (Mtb)- and Mycobacterium bovis BCG (BCG)-infected macrophages could be instrumental in understanding the characteristic post-translational modifications of host proteins and their subsequent involvement in determining Mtb pathogenesis. To identify proteins ac...

journal_title：Journal of proteome research

authors： Choudhary E,Bullen CK,Goel R,Singh AK,Praharaj M,Thakur P,Dhiman R,Bishai WR,Agarwal N

更新日期：2020-06-05 00:00:00

abstract：:Birch pollen allergy is diagnosed and treated with aqueous extracts from birch pollen, which contain a mixture of allergens and nonallergenic proteins, including large numbers of closely related sequence variants, so-called iso-allergens of the major allergen, Bet v 1. The quality of therapeutic and diagnostic allerge...

journal_title：Journal of proteome research

authors： Spiric J,Schulenborg T,Schwaben L,Engin AM,Karas M,Reuter A

更新日期：2017-10-06 00:00:00

abstract：:Protein tyrosine nitration by oxidative and nitrate stress is important in the pathogenesis of many inflammatory or aging-related diseases. Mass spectrometry analysis of protein nitrotyrosine is very challenging because the non-nitrated peptides suppress the signals of the low-abundance nitrotyrosine (NT) peptides. No...

journal_title：Journal of proteome research

authors： Zhao Y,Zhang Y,Sun H,Maroto R,Brasier AR

更新日期：2017-08-04 00:00:00

abstract：:Cyanobacteria developed efficient carbon concentrating mechanisms which significantly improve the photosynthetic performance and survival of cells under limiting CO(2) conditions. Dynamic changes of the Synechocystis proteome to CO(2) limitation were investigated using shotgun LC-MS/MS approach with isobaric tag for r...

journal_title：Journal of proteome research

authors： Battchikova N,Vainonen JP,Vorontsova N,Keranen M,Carmel D,Aro EM

更新日期：2010-11-05 00:00:00

abstract：:Flooding is a serious problem for soybeans because it reduces growth and grain yield. Proteomic and metabolomic techniques were used to examine whether mitochondrial function is altered in soybeans by flooding stress. Mitochondrial fractions were purified from the roots and hypocotyls of 4-day-old soybean seedlings th...

journal_title：Journal of proteome research

authors： Komatsu S,Yamamoto A,Nakamura T,Nouri MZ,Nanjo Y,Nishizawa K,Furukawa K

更新日期：2011-09-02 00:00:00

abstract：:The silk gland of the silkworm Bombyx mori undergoes programmed cell death (PCD) during pupal metamorphosis. On the basis of their morphological changes and the occurrence of a DNA ladder, the tissue cells were categorized into three groups: intact, committed, and dying. To identify the proteins involved in this proce...

journal_title：Journal of proteome research

authors： Jia SH,Li MW,Zhou B,Liu WB,Zhang Y,Miao XX,Zeng R,Huang YP

更新日期：2007-08-01 00:00:00