Abstract：
:The silk gland of the silkworm Bombyx mori undergoes programmed cell death (PCD) during pupal metamorphosis. On the basis of their morphological changes and the occurrence of a DNA ladder, the tissue cells were categorized into three groups: intact, committed, and dying. To identify the proteins involved in this process, we conducted a comparative proteomic analysis. Protein expression changes among the three different cell types were examined by two-dimensional gel electrophoresis. Among approximately 1000 reproducibly detected protein spots on each gel, 43 were down-regulated and 34 were up-regulated in PCD process. Mass spectrometry identified 17 differentially expressed proteins, including some well-studied proteins as well as some novel PCD related proteins, such as caspases, proteasome subunit, elongation factor, heat shock protein, and hypothetical proteins. Our results suggest that these proteins may participate in the silk gland PCD process of B. mori and, thus, provide new insights for this mechanism.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Jia SH,Li MW,Zhou B,Liu WB,Zhang Y,Miao XX,Zeng R,Huang YPdoi
10.1021/pr070043fsubject
Has Abstractpub_date
2007-08-01 00:00:00pages
3003-10issue
8eissn
1535-3893issn
1535-3907journal_volume
6pub_type
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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更新日期：2009-11-01 00:00:00
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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更新日期：2010-11-05 00:00:00
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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