Abstract：
:Sulfur trafficking systems are multiprotein systems that synthesize sulfur-containing cofactors such as iron-sulfur clusters. The sulfur is derived enzymatically from cysteine and transferred between nucleophilic cysteine residues within proteins until incorporation into the relevant cofactor. As these systems are poorly understood, we have developed an in vivo method for characterizing these interactions and have applied our method to the SUF system of Escherichia coli, which is responsible for iron-sulfur cluster biogenesis under oxidative stress and iron limitation. Proteins that interact covalently with SufE were trapped in vivo, purified, and identified by mass spectrometry. We identified SufE-SufS and SufE-SufB interactions, interactions previously demonstrated in vitro, indicating that our method has the ability to identify physiologically relevant interactions. The sulfur acceptor function of SufE is likely due to the low pK(a) of its active site C51, which we determined to be 6.3 ± 0.7. We found that SufE interacts with several Fe-S cluster proteins, further supporting the validity of the method, and with tryptophanase, glutaredoxin-3, and glutaredoxin-4, possibly suggesting a role for these enzymes in iron-sulfur biogenesis by the SUF system. Our results indicate that this method could serve as a general tool for the determination of sulfur trafficking mechanisms.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Bolstad HM,Wood MJdoi
10.1021/pr100920rsubject
Has Abstractpub_date
2010-12-03 00:00:00pages
6740-51issue
12eissn
1535-3893issn
1535-3907journal_volume
9pub_type
杂志文章abstract：:Flooding has severe negative effects on soybean growth. To explore the flooding-responsive mechanisms in early-stage soybean, a phosphoproteomic approach was used. Two-day-old soybean plants were treated without or with flooding for 3, 6, 12, and 24 h, and root tip proteins were then extracted and analyzed at each tim...
journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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更新日期：2002-11-01 00:00:00
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journal_title：Journal of proteome research
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更新日期：2008-11-01 00:00:00
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journal_title：Journal of proteome research
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更新日期：2014-11-07 00:00:00
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pub_type： 杂志文章
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更新日期：2013-03-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr4008078
更新日期：2014-02-07 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr200872s
更新日期：2012-03-02 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr3010919
更新日期：2013-03-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr5007729
更新日期：2015-01-02 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr9012078
更新日期：2010-06-04 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr100815q
更新日期：2010-11-05 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr9005662
更新日期：2009-10-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr070247w
更新日期：2007-09-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章,评审
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更新日期：2008-08-01 00:00:00
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journal_title：Journal of proteome research
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doi：10.1021/pr300123g
更新日期：2012-08-03 00:00:00
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journal_title：Journal of proteome research
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doi：10.1021/acs.jproteome.5b00474
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journal_title：Journal of proteome research
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doi：10.1021/pr1012009
更新日期：2011-07-01 00:00:00
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更新日期：2013-03-01 00:00:00
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journal_title：Journal of proteome research
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更新日期：2016-06-03 00:00:00
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journal_title：Journal of proteome research
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更新日期：2007-09-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章,评审
doi：10.1021/acs.jproteome.5b00857
更新日期：2016-01-04 00:00:00
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journal_title：Journal of proteome research
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更新日期：2019-07-05 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章,评审
doi：10.1021/pr0341282
更新日期：2004-03-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章,评审
doi：10.1021/pr2008829
更新日期：2012-01-01 00:00:00
