Abstract:
:Staphylococcus aureus with spa-type t437 has been identified as a predominant community-associated methicillin-resistant S. aureus clone from Asia, which is also encountered in Europe. Molecular typing has previously shown that t437 isolates are highly similar regardless of geographical regions or host environments. The present study was aimed at assessing to what extent this high similarity is actually reflected in the production of secreted virulence factors. We therefore profiled the extracellular proteome, representing the main reservoir of virulence factors, of 20 representative clinical isolates by mass spectrometry. The results show that these isolates can be divided into three groups and nine subgroups based on exoproteome abundance signatures. This implies that S. aureus t437 isolates show substantial exoproteome heterogeneity. Nonetheless, 30 highly conserved extracellular proteins, of which about 50% have a predicted role in pathogenesis, were dominantly identified. To approximate the virulence of the 20 investigated isolates, we employed infection models based on Galleria mellonella and HeLa cells. The results show that the grouping of clinical isolates based on their exoproteome profile can be related to virulence. We consider this outcome important as our approach provides a tool to pinpoint differences in virulence among seemingly highly similar clinical isolates of S. aureus.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Zhao X,Palma Medina LM,Stobernack T,Glasner C,de Jong A,Utari P,Setroikromo R,Quax WJ,Otto A,Becher D,Buist G,van Dijl JMdoi
10.1021/acs.jproteome.9b00179subject
Has Abstractpub_date
2019-07-05 00:00:00pages
2859-2874issue
7eissn
1535-3893issn
1535-3907journal_volume
18pub_type
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