A difference gel electrophoresis study on thylakoids isolated from poplar leaves reveals a negative impact of ozone exposure on membrane proteins.


:Populus tremula L. x P. alba L. (Populus x canescens (Aiton) Smith), clone INRA 717-1-B4, saplings were subjected to 120 ppb ozone exposure for 28 days. Chloroplasts were isolated, and the membrane proteins, solubilized using the detergent 1,2-diheptanoyl-sn-glycero-3-phosphocholine (DHPC), were analyzed in a difference gel electrophoresis (DiGE) experiment comparing control versus ozone-exposed plants. Extrinsic photosystem (PS) proteins and adenosine triphosphatase (ATPase) subunits were detected to vary in abundance. The general trend was a decrease in abundance, except for ferredoxin-NADP(+) oxidoreductase (FNR), which increased after the first 7 days of exposure. The up-regulation of FNR would increase NAPDH production for reducing power and detoxification inside and outside of the chloroplast. Later on, FNR and a number of PS and ATPase subunits decrease in abundance. This could be the result of oxidative processes on chloroplast proteins but could also be a way to down-regulate photochemical reactions in response to an inhibition in Calvin cycle activity.


J Proteome Res


Bohler S,Sergeant K,Hoffmann L,Dizengremel P,Hausman JF,Renaut J,Jolivet Y




Has Abstract


2011-07-01 00:00:00












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    authors: Queiroz RM,Charneau S,Motta FN,Santana JM,Roepstorff P,Ricart CA

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    authors: Ciborowski M,Lipska A,Godzien J,Ferrarini A,Korsak J,Radziwon P,Tomasiak M,Barbas C

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    authors: Chang C,Li L,Zhang C,Wu S,Guo K,Zi J,Chen Z,Jiang J,Ma J,Yu Q,Fan F,Qin P,Han M,Su N,Chen T,Wang K,Zhai L,Zhang T,Ying W,Xu Z,Zhang Y,Liu Y,Liu X,Zhong F,Shen H,Wang Q,Hou G,Zhao H,Li G,Liu S,G

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    journal_title:Journal of proteome research

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    authors: D'Angelo G,Chaerkady R,Yu W,Hizal DB,Hess S,Zhao W,Lekstrom K,Guo X,White WI,Roskos L,Bowen MA,Yang H

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    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Yao Y,Wang Y,Wang S,Liu X,Liu Z,Li Y,Fang Z,Mao J,Zheng Y,Ye M

    更新日期:2019-04-05 00:00:00

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    pub_type: 杂志文章


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    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Huang C,Lei H,Zhao X,Tang H,Wang Y

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    journal_title:Journal of proteome research

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    authors: Heintz D,Gallien S,Compagnon V,Berna A,Suzuki M,Yoshida S,Muranaka T,Van Dorsselaer A,Schaeffer C,Bach TJ,Schaller H

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    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Zhang M,Li H,He Y,Sun H,Xia L,Wang L,Sun B,Ma L,Zhang G,Li J,Li Y,Xie L

    更新日期:2015-07-02 00:00:00

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    pub_type: 杂志文章


    authors: Kim JW,Hwang H,Lim JS,Lee HJ,Jeong SK,Yoo JS,Paik YK

    更新日期:2016-11-04 00:00:00

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    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Darrah E,Kim A,Zhang X,Boronina T,Cole RN,Fava A,Giles JT,Bingham III CO,Chalmers MJ,Griffin PR,Sadegh-Nasseri S,Rosen A

    更新日期:2017-01-06 00:00:00

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    abstract::The enormous challenges of mass spectrometry-based metaproteomics are primarily related to the analysis and interpretation of the acquired data. This includes reliable identification of mass spectra and the meaningful integration of taxonomic and functional meta-information from samples containing hundreds of unknown ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Muth T,Behne A,Heyer R,Kohrs F,Benndorf D,Hoffmann M,Lehtevä M,Reichl U,Martens L,Rapp E

    更新日期:2015-03-06 00:00:00