Ultra-Performance Liquid Chromatography-High-Resolution Mass Spectrometry and Direct Infusion-High-Resolution Mass Spectrometry for Combined Exploratory and Targeted Metabolic Profiling of Human Urine.

abstract：:Hematopoietic stem cell transplantation is the oldest and most successful form of stem cell therapy. High dose therapy (HDT) followed by hematopoietic stem cell transplantation allows physicians to administer increased amounts of chemotherapy and/or radiation while minimizing negative side effects such as damage to bl...

journal_title：Journal of proteome research

authors： Cano KE,Li L,Bhatia S,Bhatia R,Forman SJ,Chen Y

更新日期：2011-06-03 00:00:00

abstract：:Untargeted GC-TOF-MS analysis proved to be a suitable analytical platform to determine alterations in the metabolic profile. Several metabolic pathways were found to be altered in a first clinical study comparing smokers against nonsmokers. Subsequently, we conducted a clinical diet-controlled study to investigate alt...

journal_title：Journal of proteome research

authors： Goettel M,Niessner R,Mueller D,Scherer M,Scherer G,Pluym N

更新日期：2017-10-06 00:00:00

abstract：:Cyadox is an antibiotic drug and has the potential to be used as a feedstuff additive in promoting the growth of animals. However, the toxicity of cyadox should be fully assessed before application, and this has prompted the current investigation on the metabolic responses of mice to cyadox exposure, using a metabonom...

journal_title：Journal of proteome research

authors： Huang C,Lei H,Zhao X,Tang H,Wang Y

更新日期：2013-01-04 00:00:00

abstract：:Seasonal nitrogen (N) cycling in temperate deciduous trees involves the accumulation of bark storage proteins (BSPs) in phloem parenchyma and xylem ray cells. BSPs are anabolized using recycled N during autumn leaf senescence and later become a source of N during spring shoot growth as they are catabolized. Little is ...

journal_title：Journal of proteome research

authors： Islam N,Li G,Garrett WM,Lin R,Sriram G,Cooper B,Coleman GD

更新日期：2015-02-06 00:00:00

abstract：:Peroxiredoxin V, an atypical thioredoxin peroxidase, is widely expressed in mammalian tissues. In addition, Prdx V is localized in mitochondria, peroxisome, cytosol, and the nucleus. Prdx V has been reported to protect a wide range of cellular environments as an antioxidant enzyme, and its dysfunctions may be implicat...

journal_title：Journal of proteome research

authors： Yang HY,Kwon J,Cho EJ,Choi HI,Park C,Park HR,Park SH,Chung KJ,Ryoo ZY,Cho KO,Lee TH

更新日期：2010-08-06 00:00:00

abstract：:Amphotericin B (AmB) is a polyene antifungal drug widely used for systemic fungal infections. In this study, a metabonomic method using gas chromatography-mass spectrometry (GC/MS) was developed to characterize the metabolic profiles of Candida albicans cells exposed to AmB. Thirty-one differentially produced metaboli...

journal_title：Journal of proteome research

authors： Cao Y,Zhu Z,Chen X,Yao X,Zhao L,Wang H,Yan L,Wu H,Chai Y,Jiang Y

更新日期：2013-06-07 00:00:00

abstract：:Phospholipase C gamma 1 (PLC-γ1) occupies a critically important position in the T-cell signaling pathway. While its functions as a regulator of both Ca2+ signaling and PKC-family kinases are well characterized, PLC-γ1's role in the regulation of early T-cell receptor signaling events is incompletely understood. Activ...

journal_title：Journal of proteome research

authors： Belmont J,Gu T,Mudd A,Salomon AR

更新日期：2017-08-04 00:00:00

abstract：:Many pressing medical challenges, such as diagnosing disease, enhancing directed stem-cell differentiation, and classifying cancers, have long been hindered by limitations in our ability to quantify proteins in single cells. Mass spectrometry (MS) is poised to transcend these limitations by developing powerful methods...

journal_title：Journal of proteome research

authors： Specht H,Slavov N

更新日期：2018-08-03 00:00:00

abstract：:Liquid chromatography-tandem mass spectrometry (LC-MS/MS) and multiple reaction monitoring mass spectrometry (MRM-MS) proteomics analyses were performed on eccrine sweat of healthy controls, and the results were compared with those from individuals diagnosed with schizophrenia (SZ). This is the first large scale study...

journal_title：Journal of proteome research

authors： Raiszadeh MM,Ross MM,Russo PS,Schaepper MA,Zhou W,Deng J,Ng D,Dickson A,Dickson C,Strom M,Osorio C,Soeprono T,Wulfkuhle JD,Petricoin EF,Liotta LA,Kirsch WM

更新日期：2012-04-06 00:00:00

abstract：:Hepatic fat export occurs by apolipoprotein B-100-containing lipoprotein production, whereas impaired production leads to liver steatosis. Hepatitis C virus (HCV) infection is associated to dysregulation of apoB-100 secretion and steatosis; however, the molecular mechanism by which HCV affects the apoB-100 secretion i...

journal_title：Journal of proteome research

authors： Mancone C,Montaldo C,Santangelo L,Di Giacomo C,Costa V,Amicone L,Ippolito G,Pucillo LP,Alonzi T,Tripodi M

更新日期：2012-05-04 00:00:00

abstract：:14-3-3s are phosphoserine/phosphotreonine binding proteins that play pivotal roles as regulators of multiple cellular processes in eukaryotes. The flagellated protozoan parasite Giardia duodenalis, the causing agent of giardiasis, is a valuable simplified eukaryotic model. A single 14-3-3 isoform (g14-3-3) is expresse...

journal_title：Journal of proteome research

authors： Lalle M,Camerini S,Cecchetti S,Sayadi A,Crescenzi M,Pozio E

更新日期：2012-05-04 00:00:00

abstract：:Methotrexate (MTX) is a chemotherapeutic agent that can cause a range of toxic side effects including gastrointestinal damage, hepatotoxicity, myelosuppression, and nephrotoxicity and has potentially complex interactions with the gut microbiome. Following untargeted UPLC-qtof-MS analysis of urine and fecal samples fro...

journal_title：Journal of proteome research

authors： Letertre MPM,Munjoma N,Wolfer K,Pechlivanis A,McDonald JAK,Hardwick RN,Cherrington NJ,Coen M,Nicholson JK,Hoyles L,Swann JR,Wilson ID

更新日期：2020-08-07 00:00:00

abstract：:Ovarian carcinoma is the most lethal gynecological malignancy in worldwide. The discovery of reliable marker for early detection of ovarian carcinoma is critical for increasing patient's survival because high mortality rate is associated with late diagnosis of this tumor. In the present study, we performed comparative...

journal_title：Journal of proteome research

authors： Chung KH,Lee DH,Kim Y,Kim TH,Huh JH,Chung SG,Lee S,Lee C,Ko JJ,An HJ

更新日期：2010-01-01 00:00:00

abstract：:Transient cerebral ischemia dramatically activates small ubiquitin-like modifier (SUMO2/3) conjugation. In cells exposed to 6 h of transient oxygen/glucose deprivation (OGD), a model of ischemia, SUMOylation increases profoundly between 0 and 30 min following re-oxygenation. To elucidate the effect of transient OGD on...

journal_title：Journal of proteome research

authors： Yang W,Thompson JW,Wang Z,Wang L,Sheng H,Foster MW,Moseley MA,Paschen W

更新日期：2012-02-03 00:00:00

abstract：:The term "spectral clustering" is sometimes used to refer to the clustering of mass spectrometry data. However, it also classically refers to a family of popular clustering algorithms. To avoid confusion, a more specific term could advantageously be coined. ...

journal_title：Journal of proteome research

authors： Borges H,Guibert R,Permiakova O,Burger T

更新日期：2019-01-04 00:00:00

abstract：:Ralstonia eutropha H16 is a denitrifying microorganism able to use nitrate and nitrite as terminal electron acceptors under oxygen deprivation. To identify proteins showing an altered expression pattern in response to oxygen supply, R. eutropha cells grown aerobically and anaerobically were compared in a comprehensive...

journal_title：Journal of proteome research

authors： Kohlmann Y,Pohlmann A,Schwartz E,Zühlke D,Otto A,Albrecht D,Grimmler C,Ehrenreich A,Voigt B,Becher D,Hecker M,Friedrich B,Cramm R

更新日期：2014-10-03 00:00:00

abstract：:Human saliva is a protein-rich, easily accessible source of potential local and systemic biomarkers to monitor changes that occur under pathological conditions; however, little is known about the changes in abundance associated with normal aging. In this study, we performed a comprehensive proteomic profiling of poole...

journal_title：Journal of proteome research

authors： Ambatipudi KS,Lu B,Hagen FK,Melvin JE,Yates JR

更新日期：2009-11-01 00:00:00

abstract：:Breast cancer is a leading cancer in women and is considered to be the second-most common metastatic cancer following lung cancer. An estimated 10-16% of breast cancer patients are suffering from brain metastasis, and the diagnostic cases of breast cancer brain metastasis are increasing. Nevertheless, the mechanisms b...

journal_title：Journal of proteome research

authors： Peng W,Goli M,Mirzaei P,Mechref Y

更新日期：2019-10-04 00:00:00

abstract：:Patients with triple-negative breast cancer (TNBC) are unresponsive to endocrine and anti-HER2 pharmacotherapy, limiting their therapeutic options to chemotherapy. TNBC is frequently associated with abnormalities in the PI3K/AKT/mTOR signaling pathway; drugs targeting this pathway are currently being evaluated in thes...

journal_title：Journal of proteome research

authors： Euceda LR,Hill DK,Stokke E,Hatem R,El Botty R,Bièche I,Marangoni E,Bathen TF,Moestue SA

更新日期：2017-05-05 00:00:00

abstract：:The development of the nematode Caenorhabditis elegans is a highly dynamic process. Although various studies have assessed global transcriptome changes, information on the dynamics of the proteome during ontogenesis is not available. We metabolically labeled C. elegans by using ¹⁵N ammonium chloride as a precursor in ...

journal_title：Journal of proteome research

authors： Geillinger KE,Kuhlmann K,Eisenacher M,Meyer HE,Daniel H,Spanier B

更新日期：2012-09-07 00:00:00

abstract：:Prostate cancer (PCa) is the second leading cause of cancer-related mortality in men. The prevalent diagnosis method is based on the serum prostate-specific antigen (PSA) screening test, which suffers from low specificity, overdiagnosis, and overtreatment. In this work, untargeted metabolomic profiling of age-matched ...

journal_title：Journal of proteome research

authors： Zang X,Jones CM,Long TQ,Monge ME,Zhou M,Walker LD,Mezencev R,Gray A,McDonald JF,Fernández FM

更新日期：2014-07-03 00:00:00

abstract：:Multiple reaction monitoring (MRM) is emerging as a promising technique for the detection and quantification of protein biomarkers in complex biological samples. Compared to Western blotting or enzyme assays, its high sensitivity, specificity, accuracy, assay speed, and sample throughput represent a clear advantage fo...

journal_title：Journal of proteome research

authors： Martínez-Márquez A,Morante-Carriel J,Sellés-Marchart S,Martínez-Esteso MJ,Pineda-Lucas JL,Luque I,Bru-Martínez R

更新日期：2013-12-06 00:00:00

abstract：:Clostridioides difficile-associated infection (CDI) is a health-care-associated infection caused, as the name suggests, by obligate anaerobic pathogen C. difficile and thus mainly transmitted via highly resistant endospores from one person to the other. In vivo, the spores need to germinate into cells prior to establi...

journal_title：Journal of proteome research

authors： Abhyankar WR,Zheng L,Brul S,de Koster CG,de Koning LJ

更新日期：2019-11-01 00:00:00

abstract：:Protein phosphatase 1 (PP1) catalytic subunits typically combine with other proteins that modulate their activity, direct them to distinct substrates, or serve as substrates for PP1. More than 50 PP1-interacting proteins (PIPs) have been identified so far. Given there are approximately 10 000 phosphoproteins in mammal...

journal_title：Journal of proteome research

authors： Flores-Delgado G,Liu CW,Sposto R,Berndt N

更新日期：2007-03-01 00:00:00

abstract：:The coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has impacted a large portion of the world population. From a virus genetic perspective, a recent study described what genomic data revealed about the origin and emergence of SARS-CoV-2, proposing stronger a...

journal_title：Journal of proteome research

authors： Zhang YM,Wang L,Liu XZ,Zhang H

更新日期：2020-11-06 00:00:00

abstract：:Manassantin A is a natural product that has been shown to have anticancer activity in cell-based assays, but has a largely unknown mode-of-action. Described here is the use of two different energetics-based approaches to identify protein targets of manassantin A. Using the stability of proteins from rates of oxidation...

journal_title：Journal of proteome research

authors： Geer Wallace MA,Kwon DY,Weitzel DH,Lee CT,Stephenson TN,Chi JT,Mook RA Jr,Dewhirst MW,Hong J,Fitzgerald MC

更新日期：2016-08-05 00:00:00

abstract：:beta-Cell granules contain proteins involved in fuel regulation, which when altered, contribute to metabolic disorders including diabetes mellitus. We analyzed proteins present in purified granules from the INS-1E beta-cell model. Fifty-one component proteins were identified by LC-MS/MS including hormones, granins, pr...

journal_title：Journal of proteome research

authors： Hickey AJ,Bradley JW,Skea GL,Middleditch MJ,Buchanan CM,Phillips AR,Cooper GJ

更新日期：2009-01-01 00:00:00

abstract：:Neutrophilic granulocytes play a major role in the initiation and resolution of the inflammatory response, and demonstrate significant transcriptional and translational activity. Although much was known about neutrophils prior to the introduction of proteomics, the use of MS-based methodologies has provided an unprece...

journal_title：Journal of proteome research

authors： Tomazella GG,daSilva I,Thomé CH,Greene LJ,Koehler CJ,Thiede B,Wiker HG,de Souza GA

更新日期：2010-04-05 00:00:00

abstract：:Pathogenesis of Staphylococcus aureus, an opportunistic human pathogen, is complex and involves many virulence factors including an array of surface proteins (adhesins) that promote bacterial interactions with extracellular matrix components. A better understanding of these interactions can be achieved by studying the...

journal_title：Journal of proteome research

authors： Nandakumar R,Nandakumar MP,Marten MR,Ross JM

更新日期：2005-03-01 00:00:00

abstract：:The role played by the degree of folding of protein backbones in explaining the binding energetics of protein-ligand interactions has been studied. We analyzed the protein/peptide interactions in the RNase-S system in which amino acids at two positions of the peptide S have been mutated. The global degree of folding o...

journal_title：Journal of proteome research

authors： Estrada E,Uriarte E,Vilar S

更新日期：2006-01-01 00:00:00