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Identification of novel protein biomarkers of preterm birth in human cervical-vaginal fluid.

Abstract：

:Spontaneous preterm birth (SPTB) is a major contributor to perinatal morbidity and mortality. However, the diagnosis of preterm labor (PTL) that leads to preterm birth is difficult, and there is a pressing need for improved diagnosis. We utilized multidimensional liquid chromatography-tandem mass spectrometry (LC/LC-MS/MS; MudPIT) and Fluorescence two-dimensional differential in-gel electrophoresis (2D-DIGE) to identify potential biomarkers of PTL and SPTB. MudPIT analysis identified 205 proteins in cervical-vaginal fluid (CVF), 28 of which exhibited significant differences in pairwise and progressive comparisons. Calgranulins, annexins, S100 calcium-binding protein A7, and epidermal fatty acid binding protein were abundant in CVF and differentially present in PTL and SPTB samples, as were the serum proteins alpha-1-antitrypsin, alpha1-acid glycoprotein, haptoglobin, serotransferrin, and vitamin D binding protein. 2D-DIGE identified 17 proteins that were significantly differentially present in PTL and SPTB. Immunoblotting with specific antibodies confirmed the differences and trends of selected markers. Further characterization and quantification of these markers in a larger cohort of subjects may provide the basis for new tests for the early, noninvasive positive prediction of SPTB.

journal_name

J Proteome Res

journal_title

Journal of proteome research

authors

Pereira L,Reddy AP,Jacob T,Thomas A,Schneider KA,Dasari S,Lapidus JA,Lu X,Rodland M,Roberts CT Jr,Gravett MG,Nagalla SR

doi

10.1021/pr0605421

subject

Has Abstract

pub_date

2007-04-01 00:00:00

pages

1269-76

issue

4

eissn

1535-3893

issn

1535-3907

journal_volume

6

pub_type

杂志文章

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