Abstract：
:A metabonomic study was performed to investigate the biochemical perturbation of the serum samples from liver failure patients induced by hepatitis B virus (HBV; n=24) and control normal subjects (n=23). The serum metabonome was detected using gas chromatography-mass spectrometry (GC-MS) technique integrated with a commercial mass spectral library for the peak identification. After peak deconvolution, identification, and matching, the acquired GC-MS data were normalized and processed by principal component analysis (PCA) and canonical discriminant analysis (CDA). Specific changes in the metabolic composition of serum samples from patients including amino acids (AAs) and glucose were shown in GC-MS total ion current (TIC) chromatograms. The distinctive biochemical difference between the healthy subjects and liver failure patients was displayed by the pattern recognition methods. We also found that the liver failure patients with different degree of severity categorized as MELD (model for end-stage of liver diseases) could be clearly classified by the corresponding metabonomic data. In comparison, the current routine clinical indices cannot characterize the global phenotyping of liver failure. The result demonstrated that the GC-MS technique is an alternative tool for the characterization of the metabolic perturbation and the metabonomic study promises to provide an integrative criterion to evaluate the severity and the prognosis of liver diseases.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Yu K,Sheng G,Sheng J,Chen Y,Xu W,Liu X,Cao H,Qu H,Cheng Y,Li Ldoi
10.1021/pr060591dsubject
Has Abstractpub_date
2007-07-01 00:00:00pages
2413-9issue
7eissn
1535-3893issn
1535-3907journal_volume
6pub_type
杂志文章abstract：:The abundant proteins in human milk have been well characterized and are known to provide nutritional, protective, and developmental advantages to both term and preterm infants. However, relatively little is known about the expression of the low abundance proteins that are present in human milk because of the technica...
journal_title：Journal of proteome research
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pub_type： 杂志文章
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pub_type： 杂志文章,评审
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更新日期：2014-08-01 00:00:00
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更新日期：2009-05-01 00:00:00
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更新日期：2012-04-06 00:00:00
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更新日期：2008-09-01 00:00:00
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pub_type： 杂志文章
doi：10.1021/pr060157b
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr101082c
更新日期：2011-05-06 00:00:00
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pub_type： 杂志文章
doi：10.1021/pr060550h
更新日期：2007-05-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr0605421
更新日期：2007-04-01 00:00:00
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pub_type： 杂志文章
doi：10.1021/acs.jproteome.0c00778
更新日期：2021-01-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2008-11-01 00:00:00
abstract：:Drug resistance is one of the major causes of cancer chemotherapy failure. In the current study, we used a pair of lung adenocarcinoma cell lines, A549 and the pemetrexed-resistant A549/PEM cells, as a model to monitor resistance-dependent cellular responses and identify potential therapeutic targets. By means of 2D d...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.5b00794
更新日期：2015-11-06 00:00:00
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更新日期：2010-05-07 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2004-03-01 00:00:00
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更新日期：2015-05-01 00:00:00
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更新日期：2018-08-03 00:00:00
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更新日期：2005-03-01 00:00:00
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更新日期：2006-10-01 00:00:00
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更新日期：2012-06-01 00:00:00
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