Abstract:
:The deuterium, a frequently used stable isotope in isotopic labeling for quantitative proteomics, could deteriorate the accuracy and precision of proteome quantification owing to the retention time shift of deuterated peptides from the hydrogenated counterpart. We introduce a novel three-plexed peptide "diethylation" using only 13C isotopologues of acetaldehyde and demonstrate that the accuracy and precision of our method in proteome quantification are significantly superior to the conventional deuterium-based dimethylation labeling in both a single-shot and multidimensional LC-MS/MS analysis of the HeLa proteome. Furthermore, in time-resolved profiling of Xenopus laevis early embryogenesis, our 3-plexed diethylation outperformed isobaric labeling approaches in terms of the quantification accuracy or the number of protein identifications, generating more than two times more differentially expressed proteins. Our cost-effective and highly accurate 3-plexed diethylation method could contribute to various types of quantitative proteomics applications in which three of multiplexity would be sufficient.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Jung J,Jeong K,Choi Y,Kim SA,Kim H,Lee JW,Kim VN,Kim KP,Kim JSdoi
10.1021/acs.jproteome.8b00775subject
Has Abstractpub_date
2019-03-01 00:00:00pages
1078-1087issue
3eissn
1535-3893issn
1535-3907journal_volume
18pub_type
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