Proteomic Analysis of Brain and Cerebrospinal Fluid from the Three Major Forms of Neuronal Ceroid Lipofuscinosis Reveals Potential Biomarkers.

abstract：:Schistosomes are blood-dwelling helminth parasites that cause schistosomiasis, a debilitating disease resulting in inflammation and, in extreme cases, multiple organ damage. Major challenges to control the transmission persist, and the discovery of protective antigens remains of critical importance for vaccine develop...

journal_title：Journal of proteome research

authors： Neves LX,Wilson RA,Brownridge P,Harman VM,Holman SW,Beynon RJ,Eyers CE,DeMarco R,Castro-Borges W

更新日期：2020-01-03 00:00:00

abstract：:Acinetobacter baumannii causes severe infections in compromised patients. We combined SDS-PAGE, two-dimensional gel electrophoresis and mass spectrometry (LC-MS/MS and MALDI-TOF) to separate and characterize the proteins of the cell envelope of this bacterium. In total, 135 proteins (inner and outer membrane proteins)...

journal_title：Journal of proteome research

authors： Siroy A,Cosette P,Seyer D,Lemaître-Guillier C,Vallenet D,Van Dorsselaer A,Boyer-Mariotte S,Jouenne T,Dé E

更新日期：2006-12-01 00:00:00

abstract：:The Lewis x (Le(x)) structure (Galβ1-4(Fucα1-3)GlcNAc-R) is a carbohydrate epitope comprising the stage-specific embryonic antigen-1 (SSEA-1) and CD15, and it is synthesized by α1,3-fucosyltransferase 9 (Fut9). Fut9 is expressed specifically in the stomach, kidney, brain, and in leukocytes, suggesting a specific funct...

journal_title：Journal of proteome research

authors： Noro E,Togayachi A,Sato T,Tomioka A,Fujita M,Sukegawa M,Suzuki N,Kaji H,Narimatsu H

更新日期：2015-09-04 00:00:00

abstract：:Wilson's Disease (WD), a copper transport disorder caused by a genetic defect in the ATP7B gene, has been a long time strong candidate for newborn screening (NBS), since early interventions can give better results by preventing irreversible neurological disability or liver cirrhosis. Several previous pilot studies mea...

journal_title：Journal of proteome research

authors： Jung S,Whiteaker JR,Zhao L,Yoo HW,Paulovich AG,Hahn SH

更新日期：2017-02-03 00:00:00

abstract：:Cripto is one of the key regulators of embryonic stem cells (ESCs) differentiation into cardiomyocites vs neuronal fate. Cripto(-/-) murine ESCs have been utilized to investigate the molecular mechanisms underlying early events of mammalian lineage differentiation. 2D/LC-MS/MS and a label-free LC-MS approaches were us...

journal_title：Journal of proteome research

authors： Chambery A,Vissers JP,Langridge JI,Lonardo E,Minchiotti G,Ruvo M,Parente A

更新日期：2009-02-01 00:00:00

abstract：:Neuroblastoma (NB) is an infant tumor which frequently differentiates into neurons. We used two-dimensional differential in-gel electrophoresis (2D-DIGE) to analyze the cytosolic and nuclear protein expression patterns of LAN-5 cells following neuronal differentiating agent all-trans-retinoic acid treatment. We identi...

journal_title：Journal of proteome research

authors： Cimmino F,Spano D,Capasso M,Zambrano N,Russo R,Zollo M,Iolascon A

更新日期：2007-07-01 00:00:00

abstract：:We undertook an unbiased metabolite profiling of fibroblasts from schizophrenia patients and healthy controls to identify metabolites and pathways that are dysregulated in disease, seeking to gain new insights into the disease biology of schizophrenia and to discover potential disease-related biomarkers. We measured p...

journal_title：Journal of proteome research

authors： Huang JH,Park H,Iaconelli J,Berkovitch SS,Watmuff B,McPhie D,Öngür D,Cohen BM,Clish CB,Karmacharya R

更新日期：2017-02-03 00:00:00

abstract：:In Thailand, diabetes mellitus is the most significant risk factor for melioidosis, a severe disease caused by Burkholderia pseudomallei. In this study, neutrophils isolated from healthy or diabetic subjects were infected with B. thailandensis E555, a variant strain with a B. pseudomallei-like capsular polysaccharide ...

journal_title：Journal of proteome research

authors： Withatanung P,Kurian D,Tangjittipokin W,Plengvidhya N,Titball RW,Korbsrisate S,Stevens JM

更新日期：2019-07-05 00:00:00

abstract：:Patients with triple-negative breast cancer (TNBC) are unresponsive to endocrine and anti-HER2 pharmacotherapy, limiting their therapeutic options to chemotherapy. TNBC is frequently associated with abnormalities in the PI3K/AKT/mTOR signaling pathway; drugs targeting this pathway are currently being evaluated in thes...

journal_title：Journal of proteome research

authors： Euceda LR,Hill DK,Stokke E,Hatem R,El Botty R,Bièche I,Marangoni E,Bathen TF,Moestue SA

更新日期：2017-05-05 00:00:00

abstract：:The purpose of this study was to determine the system-wide consequences of deficiencies in two essential micronutrients, vitamins E and C, on the proteome using zebrafish (Danio rerio) as one of the few vertebrate models that similar to humans cannot synthesize vitamin C. We describe a label-free proteomics workflow t...

journal_title：Journal of proteome research

authors： Motorykin I,Traber MG,Tanguay RL,Maier CS

更新日期：2014-03-07 00:00:00

abstract：:Catechol estrogens (CEs) are known to be toxic metabolites and the initiators of the oncogenesis of breast cancers via forming covalent adducts with DNAs. CEs shall also react with proteins, but their cellular protein targets remain unexplored. Here, we reported the identification of protein targets of CEs in the solu...

journal_title：Journal of proteome research

authors： Do QT,Huang TE,Liu YC,Tai JH,Chen SH

更新日期：2021-01-01 00:00:00

abstract：:Antibodies are a primary research tool for a diverse range of experiments in biology, from development to pathology. Their utility is derived from their ability to specifically identify proteins at a high level of sensitivity. This diversity of experimental requirements stretches the capabilities of these key research...

journal_title：Journal of proteome research

authors： Haslam NJ,Gibson TJ

更新日期：2010-07-02 00:00:00

abstract：:Dendritic cells (DCs), the most potent and specialized antigen-presenting cells, play a key role in the regulation of the adaptive immunity. Immature DCs were generated by in vitro culturing of peripheral blood monocytes and functionally activated with the classical pathogen-associated molecular pattern lipopolysaccha...

journal_title：Journal of proteome research

authors： Gundacker NC,Haudek VJ,Wimmer H,Slany A,Griss J,Bochkov V,Zielinski C,Wagner O,Stöckl J,Gerner C

更新日期：2009-06-01 00:00:00

abstract：:Flooding has severe negative effects on soybean growth. To explore the flooding-responsive mechanisms in early-stage soybean, a phosphoproteomic approach was used. Two-day-old soybean plants were treated without or with flooding for 3, 6, 12, and 24 h, and root tip proteins were then extracted and analyzed at each tim...

journal_title：Journal of proteome research

authors： Yin X,Sakata K,Komatsu S

更新日期：2014-12-05 00:00:00

abstract：:Recently, we reported comparative analysis of glycoproteins which express cancer-specific N-glycans on various cancer cells and identified 24 glycoproteins having polylactosamine (polyLacNAc)-type N-glycans that are abundantly present in malignant cells [ Mitsui et al., J. Pharm. Biomed. Anal. 2012 , 70 , 718 - 726 ]....

journal_title：Journal of proteome research

authors： Kinoshita M,Mitsui Y,Kakoi N,Yamada K,Hayakawa T,Kakehi K

更新日期：2014-02-07 00:00:00

abstract：:The Esx/WXG-100 (ESAT-6/Wss) exporters are multiprotein complexes that promote protein translocation across the cytoplasmic membrane in a diverse range of pathogenic and nonpathogenic bacterial species. The Esx-1 (ESAT-6 System-1) system mediates virulence factor translocation in mycobacterial pathogens, including the...

journal_title：Journal of proteome research

authors： Champion MM,Williams EA,Pinapati RS,Champion PA

更新日期：2014-11-07 00:00:00

abstract：:A popular method for peptide quantification relies on isobaric labeling such as tandem mass tags (TMT), which enables multiplexed proteome analyses. Quantification is achieved by reporter ions generated by fragmentation in a tandem mass spectrometer. However, with higher degrees of multiplexing, the smaller mass diffe...

journal_title：Journal of proteome research

authors： Kelstrup CD,Aizikov K,Batth TS,Kreutzman A,Grinfeld D,Lange O,Mourad D,Makarov AA,Olsen JV

更新日期：2018-11-02 00:00:00

abstract：:We have studied the role(s) of maturation drying in the acquisition of germinability, seedling vigor and pathogen resistance by comparing the proteome changes in maize embryo and endosperm during mature and prematurely imposed drying. Prematurely imposed dried seeds at 40 days after pollination (DAP) germinated almost...

journal_title：Journal of proteome research

authors： Wang WQ,Ye JQ,Rogowska-Wrzesinska A,Wojdyla KI,Jensen ON,Møller IM,Song SQ

更新日期：2014-02-07 00:00:00

abstract：:Activation of AMP-activated protein kinase (AMPK) upon energy depletion stimulates energy production and limits energy utilization. Erythrocytes lacking AMPK are susceptible to suicidal cell death (eryptosis). A hallmark of eryptosis is cell membrane scrambling with phosphatidylserine exposure at the erythrocyte surfa...

journal_title：Journal of proteome research

authors： Zelenak C,Föller M,Velic A,Krug K,Qadri SM,Viollet B,Lang F,Macek B

更新日期：2011-04-01 00:00:00

abstract：:Cytological examination of cells from bronchoalveolar lavage (BAL) is commonly used for the diagnosis of lung cancer. Proteins released from lung cancer cells into BAL may serve as biomarkers for cancer detection. In this study, N-glycoproteins in eight cases of BAL fluid, as well as eight lung adenocarcinoma tissues ...

journal_title：Journal of proteome research

authors： Li QK,Shah P,Li Y,Aiyetan PO,Chen J,Yung R,Molena D,Gabrielson E,Askin F,Chan DW,Zhang H

更新日期：2013-08-02 00:00:00

abstract：:POPX2 is a serine/threonine phosphatase belonging to the protein phosphatase 2C (PP2C) family that has been found to be elevated in invasive breast cancer cells. Silencing of POPX2 results in lower cell motility and invasiveness. The molecular mechanism of POPX2-regulated cell motility is not well understood. To ident...

journal_title：Journal of proteome research

authors： Zhang S,Guo T,Chan H,Sze SK,Koh CG

更新日期：2013-06-07 00:00:00

abstract：:Prediction of the types of membrane proteins is of great importance both for genome-wide annotation and for experimental researchers to understand proteins' functions. We describe a new strategy for the prediction of the types of membrane proteins using the Nearest Neighbor Algorithm. We introduced a bipartite feature...

journal_title：Journal of proteome research

authors： Jia P,Qian Z,Feng K,Lu W,Li Y,Cai Y

更新日期：2008-03-01 00:00:00

abstract：:Arabidopsis MAP kinase 4 (MPK4) has been proposed to be a negative player in plant immunity, and it is also activated by pathogen-associated molecular patterns (PAMPs), such as flg22. The molecular mechanisms by which MPK4 is activated and regulates plant defense remain elusive. In this study, we investigated Arabidop...

journal_title：Journal of proteome research

authors： Zhang T,Schneider JD,Lin C,Geng S,Ma T,Lawrence SR,Dufresne CP,Harmon AC,Chen S

更新日期：2019-03-01 00:00:00

abstract：:Streptococcus agalactiae causes severe systemic infections in human and fish. In the present study, we established a pathogen-plasma interaction model by which we explored how S. agalactiae evaded serum-mediated killing. We found that S. agalactiae grew faster in the presence of yellow grouper plasma than in the absen...

journal_title：Journal of proteome research

authors： Wang Z,Li MY,Peng B,Cheng ZX,Li H,Peng XX

更新日期：2016-07-01 00:00:00

abstract：:We have investigated the transcriptome and proteome of the venom of a cryptic Australian elapid snake Drysdalia coronoides. To probe into the transcriptome, we constructed a partial cDNA library from the venom gland of D. coronoides. The proteome of the venom of D. coronoides was explored by tryptic digestion of the c...

journal_title：Journal of proteome research

authors： Chatrath ST,Chapeaurouge A,Lin Q,Lim TK,Dunstan N,Mirtschin P,Kumar PP,Kini RM

更新日期：2011-02-04 00:00:00

abstract：:Lysine methylation plays important roles in structural and functional regulation of chromatin. Although trypsin is the most widely used protease in mass spectrometry-based proteomic analysis for lysine methylation substrates, the proteolytic activity of trypsin on dimethylated lysine residues remains an arguable issue...

journal_title：Journal of proteome research

authors： Chen M,Zhang M,Zhai L,Hu H,Liu P,Tan M

更新日期：2017-09-01 00:00:00

abstract：:A proper sample preparation, in particular, abundant protein removal is crucial in the characterization of low-abundance proteins including those harboring post-translational modifications. In human cerebrospinal fluid (CSF), approximately 80% of proteins originate from serum, and removal of major proteins is necessar...

journal_title：Journal of proteome research

authors： Ogata Y,Charlesworth MC,Muddiman DC

更新日期：2005-05-01 00:00:00

abstract：:Protein cleavage coupled with isotope dilution mass spectrometry (PC-IDMS) has the potential to provide the absolute concentration of a specific protein, or multiple proteins, in complex mixtures. However, PC-IDMS differs from standard IDMS since the internal standard is a different molecule than the analyte at the st...

journal_title：Journal of proteome research

authors： Barnidge DR,Hall GD,Stocker JL,Muddiman DC

更新日期：2004-05-01 00:00:00

abstract：:Protein chemical cross-linking combined with mass spectrometry has become an important technique for the analysis of protein structure and protein-protein interactions. A variety of cross-linkers are well developed, but reliable, rapid, and user-friendly tools for large-scale analysis of cross-linked proteins are stil...

journal_title：Journal of proteome research

authors： Lu L,Millikin RJ,Solntsev SK,Rolfs Z,Scalf M,Shortreed MR,Smith LM

更新日期：2018-07-06 00:00:00

abstract：:Comparative phosphoproteomics of Mycobacterium tuberculosis (Mtb)- and Mycobacterium bovis BCG (BCG)-infected macrophages could be instrumental in understanding the characteristic post-translational modifications of host proteins and their subsequent involvement in determining Mtb pathogenesis. To identify proteins ac...

journal_title：Journal of proteome research

authors： Choudhary E,Bullen CK,Goel R,Singh AK,Praharaj M,Thakur P,Dhiman R,Bishai WR,Agarwal N

更新日期：2020-06-05 00:00:00