Abstract：
:We undertook an unbiased metabolite profiling of fibroblasts from schizophrenia patients and healthy controls to identify metabolites and pathways that are dysregulated in disease, seeking to gain new insights into the disease biology of schizophrenia and to discover potential disease-related biomarkers. We measured polar and nonpolar metabolites in the fibroblasts under normal conditions and under two stressful physiological perturbations: growth in low-glucose media and exposure to the steroid hormone dexamethasone. We found that metabolites that were significantly different between schizophrenia and control subjects showed separation of the two groups by partial least-squares discriminant analysis methods. This separation between schizophrenia and healthy controls was more robust with metabolites identified under the perturbation conditions. The most significant individual metabolite differences were also found in the perturbation experiments. Metabolites that were significantly different between schizophrenia and healthy controls included a number of plasmalogens and phosphatidylcholines. We present these results in the context of previous reports of metabolic profiling of brain tissue and plasma in schizophrenia. These results show the applicability of metabolite profiling under stressful perturbations to reveal cellular pathways that may be involved in disease biology.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Huang JH,Park H,Iaconelli J,Berkovitch SS,Watmuff B,McPhie D,Öngür D,Cohen BM,Clish CB,Karmacharya Rdoi
10.1021/acs.jproteome.6b00628subject
Has Abstractpub_date
2017-02-03 00:00:00pages
481-493issue
2eissn
1535-3893issn
1535-3907journal_volume
16pub_type
杂志文章abstract：:RelA (NF-kappaB) is a transcription factor inducible by distinct stimuli in many different cell types. To find new cell type specific cofactors of NF-kappaB dependent transcription, we isolated RelA transcription activation domain binding proteins from the nuclear extracts of three different cell types. Analysis by el...
journal_title：Journal of proteome research
pub_type： 杂志文章
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2004-01-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2008-12-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2006-11-01 00:00:00
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journal_title：Journal of proteome research
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更新日期：2012-07-06 00:00:00
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journal_title：Journal of proteome research
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更新日期：2016-07-01 00:00:00
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pub_type： 杂志文章
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更新日期：2010-10-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr060367o
更新日期：2007-01-01 00:00:00
abstract：:Mixed lineage leukemia results from chromosomal rearrangements of the gene mixed lineage leukemia (MLL). MLL-AF9 is one such rearrangement that recruits the lysine methyltransferase, human disruptor of telomere silencing 1-like (DOT1L) and lysine specific demethylase 1 (LSD1), resulting in elevated expression of the H...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.8b00118
更新日期：2018-08-03 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr060668y
更新日期：2007-06-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2010-04-05 00:00:00
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journal_title：Journal of proteome research
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doi：10.1021/pr0703627
更新日期：2007-11-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr300893w
更新日期：2013-01-04 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr801064u
更新日期：2009-04-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2012-05-04 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2016-12-02 00:00:00
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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更新日期：2009-11-01 00:00:00
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journal_title：Journal of proteome research
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更新日期：2017-02-03 00:00:00
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journal_title：Journal of proteome research
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更新日期：2014-05-02 00:00:00
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journal_title：Journal of proteome research
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更新日期：2009-10-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2009-09-01 00:00:00
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