Retinal Mueller glial cells trigger the hallmark inflammatory process in autoimmune uveitis.

abstract：:Human embryonic stem cells (hESCs) are promising in regenerative medicine (RM) due to their differentiation plasticity and proliferation potential. However, a major challenge in RM is the generation of a vascular system to support nutrient flow to newly synthesized tissues. Here we refined an existing method to genera...

journal_title：Journal of proteome research

authors： Tsolis KC,Bagli E,Kanaki K,Zografou S,Carpentier S,Bei ES,Christoforidis S,Zervakis M,Murphy C,Fotsis T,Economou A

更新日期：2016-06-03 00:00:00

abstract：:Comparative phosphoproteomics of Mycobacterium tuberculosis (Mtb)- and Mycobacterium bovis BCG (BCG)-infected macrophages could be instrumental in understanding the characteristic post-translational modifications of host proteins and their subsequent involvement in determining Mtb pathogenesis. To identify proteins ac...

journal_title：Journal of proteome research

authors： Choudhary E,Bullen CK,Goel R,Singh AK,Praharaj M,Thakur P,Dhiman R,Bishai WR,Agarwal N

更新日期：2020-06-05 00:00:00

abstract：:Beta-thalassemia results from mutations of the β-hemoglobin (Hbb) gene and reduced functional Hbb synthesis. Excess α-Hb causes globin chain aggregation, oxidation, cytoskeletal damage, and increased red blood cell clearance. These events result in anemia, altered iron homeostasis, and expansion of extramedullary eryt...

journal_title：Journal of proteome research

authors： Vallelian F,Gelderman-Fuhrmann MP,Schaer CA,Puglia M,Opitz L,Baek JH,Vostal J,Buehler PW,Schaer DJ

更新日期：2015-02-06 00:00:00

abstract：:Protein phosphorylation is the most abundant reversible covalent modification. Human protein kinases participate in almost all biological pathways, and approximately half of the kinases are associated with disease. PhoSigNet was designed to store and display human phosphorylation-mediated signal transduction networks,...

journal_title：Journal of proteome research

authors： Zhang M,Li H,He Y,Sun H,Xia L,Wang L,Sun B,Ma L,Zhang G,Li J,Li Y,Xie L

更新日期：2015-07-02 00:00:00

abstract：:Using an integrated transcriptomic and proteomic approach, we characterized the venom peptidome of the European red ant, Manica rubida. We identified 13 "myrmicitoxins" that share sequence similarities with previously identified ant venom peptides, one of them being identified as an EGF-like toxin likely resulting fro...

journal_title：Journal of proteome research

authors： Touchard A,Aili SR,Téné N,Barassé V,Klopp C,Dejean A,Kini RM,Mrinalini,Coquet L,Jouenne T,Lefranc B,Leprince J,Escoubas P,Nicholson GM,Treilhou M,Bonnafé E

更新日期：2020-04-03 00:00:00

abstract：:Birch pollen allergy is diagnosed and treated with aqueous extracts from birch pollen, which contain a mixture of allergens and nonallergenic proteins, including large numbers of closely related sequence variants, so-called iso-allergens of the major allergen, Bet v 1. The quality of therapeutic and diagnostic allerge...

journal_title：Journal of proteome research

authors： Spiric J,Schulenborg T,Schwaben L,Engin AM,Karas M,Reuter A

更新日期：2017-10-06 00:00:00

abstract：:Aggregated and protease-resistant mammalian prion protein (PrPSc) is the primary protein component of infectious prions. Enriched PrPSc preparations are often used to study the mechanisms that underly prion disease. However, most enrichment procedures are relatively nonspecific and tend to yield significant amounts of...

journal_title：Journal of proteome research

authors： Moore RA,Choi YP,Head MW,Ironside JW,Faris R,Ritchie DL,Zanusso G,Priola SA

更新日期：2016-12-02 00:00:00

abstract：:While numerous strategies exist for biomarker discovery, the bottleneck to product development and routine use at the clinic is in the verification phase of candidate biomarkers. The aim of this study was to establish a robust and high-throughput product ion monitoring (PIM) assay that is potentially capable of rapidl...

journal_title：Journal of proteome research

authors： Kulasingam V,Smith CR,Batruch I,Buckler A,Jeffery DA,Diamandis EP

更新日期：2008-02-01 00:00:00

abstract：:The Model Organism Protein Expression Database (MOPED, http://moped.proteinspire.org) is an expanding proteomics resource to enable biological and biomedical discoveries. MOPED aggregates simple, standardized and consistently processed summaries of protein expression and metadata from proteomics (mass spectrometry) ex...

journal_title：Journal of proteome research

authors： Higdon R,Stewart E,Stanberry L,Haynes W,Choiniere J,Montague E,Anderson N,Yandl G,Janko I,Broomall W,Fishilevich S,Lancet D,Kolker N,Kolker E

更新日期：2014-01-03 00:00:00

abstract：:Depletion of DNA replication initiation factors such as CDC7 kinase triggers the origin activation checkpoint in healthy cells and leads to a protective cell cycle arrest at the G1 phase of the mitotic cell division cycle. This protective mechanism is thought to be defective in cancer cells. To investigate how this ch...

journal_title：Journal of proteome research

authors： Mulvey CM,Tudzarova S,Crawford M,Williams GH,Stoeber K,Godovac-Zimmermann J

更新日期：2013-03-01 00:00:00

abstract：:The development of the nematode Caenorhabditis elegans is a highly dynamic process. Although various studies have assessed global transcriptome changes, information on the dynamics of the proteome during ontogenesis is not available. We metabolically labeled C. elegans by using ¹⁵N ammonium chloride as a precursor in ...

journal_title：Journal of proteome research

authors： Geillinger KE,Kuhlmann K,Eisenacher M,Meyer HE,Daniel H,Spanier B

更新日期：2012-09-07 00:00:00

abstract：:Mammary tumors are a major health threat to women and female dogs. In both, metastasis of the primary tumor to distant organs is the most common cause of tumor-related death. Nevertheless, the molecular mechanisms of tumor metastasis are far from being understood, and it is still unknown why some human and canine carc...

journal_title：Journal of proteome research

authors： Klopfleisch R,Klose P,Weise C,Bondzio A,Multhaup G,Einspanier R,Gruber AD

更新日期：2010-12-03 00:00:00

abstract：:High-resolution proton nuclear magnetic resonance (NMR) spectrometry of biofluids has been increasingly used in laboratory diagnosis of various diseases. In this study, we extended the use of (1)H NMR spectroscopy for laboratory diagnosis of exudative pleural effusions using pleural fluids. We compared this new NMR-ba...

journal_title：Journal of proteome research

authors： Lam CW,Law CY

更新日期：2014-09-05 00:00:00

abstract：:Early findings propose that impaired neurotransmission in the brain plays a key role in the pathophysiology of schizophrenia. Recent advances in understanding its multiple etiologies and pathogenetic mechanisms provide more speculative hypotheses focused on even broader somatic systems. Using a targeted tandem mass sp...

journal_title：Journal of proteome research

authors： Cai HL,Li HD,Yan XZ,Sun B,Zhang Q,Yan M,Zhang WY,Jiang P,Zhu RH,Liu YP,Fang PF,Xu P,Yuan HY,Zhang XH,Hu L,Yang W,Ye HS

更新日期：2012-08-03 00:00:00

abstract：:Sensitive detection of molecular biomarkers in clinical samples is crucially important in disease diagnostics. This paper reports the development of an aptamer microarray platform combined with sol-gel technology to identify low-abundance targets in complex serum samples. Because of the nanoporous structure of the sol...

journal_title：Journal of proteome research

authors： Ahn JY,Lee SW,Kang HS,Jo M,Lee DK,Laurell T,Kim S

更新日期：2010-11-05 00:00:00

abstract：:A fast and robust method for lipid profiling utilizing liquid chromatography coupled with mass spectrometry has been demonstrated and validated for the analysis of human plasma. This method allowed quantification and identification of lipids in human plasma using parallel alternating low energy and high energy collisi...

journal_title：Journal of proteome research

authors： Castro-Perez JM,Kamphorst J,DeGroot J,Lafeber F,Goshawk J,Yu K,Shockcor JP,Vreeken RJ,Hankemeier T

更新日期：2010-05-07 00:00:00

abstract：:We have expanded our recent on-line LC-MS platform for large peptide analysis to combine collision-induced dissociation (CID), electron-transfer dissociation (ETD), and CID of an isolated charge-reduced (CRCID) species derived from ETD to determine sites of phosphorylation and glycosylation modifications, as well as t...

journal_title：Journal of proteome research

authors： Wu SL,Hühmer AF,Hao Z,Karger BL

更新日期：2007-11-01 00:00:00

abstract：:Grapes are among the widely cultivated fruit crops in the world. Grape berries like other nonclimacteric fruits undergo a complex set of dynamic, physical, physiological, and biochemical changes during ripening. Muscadine grapes are widely cultivated in the southern United States for fresh fruit and wine. To date, cha...

journal_title：Journal of proteome research

authors： Kambiranda D,Katam R,Basha SM,Siebert S

更新日期：2014-02-07 00:00:00

abstract：:The current report describes the use of CapLC-ESI-Q/TOF-MS for investigating the proteome profiles of hypertonic saline-induced sputum samples from 56 smokers. The severity of their lung disease ranged from normal (healthy smokers) to chronic bronchitis, chronic obstructive pulmonary disease (COPD), and COPD with emph...

journal_title：Journal of proteome research

authors： Casado B,Iadarola P,Pannell LK,Luisetti M,Corsico A,Ansaldo E,Ferrarotti I,Boschetto P,Baraniuk JN

更新日期：2007-12-01 00:00:00

abstract：:The deuterium, a frequently used stable isotope in isotopic labeling for quantitative proteomics, could deteriorate the accuracy and precision of proteome quantification owing to the retention time shift of deuterated peptides from the hydrogenated counterpart. We introduce a novel three-plexed peptide "diethylation" ...

journal_title：Journal of proteome research

authors： Jung J,Jeong K,Choi Y,Kim SA,Kim H,Lee JW,Kim VN,Kim KP,Kim JS

更新日期：2019-03-01 00:00:00

abstract：:Histones regulate a variety of chromatin templated events by their post-translational modifications (PTMs). Although there are extensive reports on the PTMs of canonical histones, the information on the histone variants remains very scanty. Here, we report the identification of different PTMs, such as acetylation, met...

journal_title：Journal of proteome research

authors： Pentakota SK,Sandhya S,P Sikarwar A,Chandra N,Satyanarayana Rao MR

更新日期：2014-12-05 00:00:00

abstract：:A number of aptamers have been selected against cell surface biomarkers or against eukaryotic tissue culture cells themselves. To determine the general utility of aptamers for assessing the cell surface proteome, we developed a standardized flow cytometry assay and carried out a comprehensive study with 7 different ap...

journal_title：Journal of proteome research

authors： Li N,Ebright JN,Stovall GM,Chen X,Nguyen HH,Singh A,Syrett A,Ellington AD

更新日期：2009-05-01 00:00:00

abstract：:The final goal of the Russian part of the Chromosome-centric Human Proteome Project (C-HPP) was established as the analysis of the chromosome 18 (Chr 18) protein complement in plasma, liver tissue and HepG2 cells with the sensitivity of 10(-18) M. Using SRM, we have recently targeted 277 Chr 18 proteins in plasma, liv...

journal_title：Journal of proteome research

authors： Zgoda VG,Kopylov AT,Tikhonova OV,Moisa AA,Pyndyk NV,Farafonova TE,Novikova SE,Lisitsa AV,Ponomarenko EA,Poverennaya EV,Radko SP,Khmeleva SA,Kurbatov LK,Filimonov AD,Bogolyubova NA,Ilgisonis EV,Chernobrovkin AL,Ivanov AS

更新日期：2013-01-04 00:00:00

abstract：:Hypoxia inducible factor (HIF)-2α protein expression in solid tumors promotes stem-like phenotype in cancer stem cells and increases tumorigenic potential in nonstem cancer cells. Recently, we have shown that HIF-1/2α gene expression is correlated to neuroblastoma (NB) poor survival and to undifferentiated tumor state...

journal_title：Journal of proteome research

authors： Cimmino F,Pezone L,Avitabile M,Persano L,Vitale M,Sassi M,Bresolin S,Serafin V,Zambrano N,Scaloni A,Basso G,Iolascon A,Capasso M

更新日期：2016-10-07 00:00:00

abstract：:Schistosomes are blood-dwelling helminth parasites that cause schistosomiasis, a debilitating disease resulting in inflammation and, in extreme cases, multiple organ damage. Major challenges to control the transmission persist, and the discovery of protective antigens remains of critical importance for vaccine develop...

journal_title：Journal of proteome research

authors： Neves LX,Wilson RA,Brownridge P,Harman VM,Holman SW,Beynon RJ,Eyers CE,DeMarco R,Castro-Borges W

更新日期：2020-01-03 00:00:00

abstract：:Proteomics of human milk has been used to identify the comprehensive cargo of proteins involved in immune and cellular function. Very little is known about the effects of gestational diabetes mellitus (GDM) on lactation and breast milk components. The objective of the current study was to examine the effect of GDM on ...

journal_title：Journal of proteome research

authors： Grapov D,Lemay DG,Weber D,Phinney BS,Azulay Chertok IR,Gho DS,German JB,Smilowitz JT

更新日期：2015-01-02 00:00:00

abstract：:Sterols are membrane-bound isoprenoid lipids that are required for cell viability and growth. In plants, it is generally assumed that 3-hydroxy-3-methylglutaryl-CoA-reductase (HMGR) is a key element of their biosynthesis, but the molecular regulation of that pathway is largely unknown. In an attempt to identify regula...

journal_title：Journal of proteome research

authors： Heintz D,Gallien S,Compagnon V,Berna A,Suzuki M,Yoshida S,Muranaka T,Van Dorsselaer A,Schaeffer C,Bach TJ,Schaller H

更新日期：2012-02-03 00:00:00

abstract：:The majority of mitochondrial preproteins are targeted via N-terminal presequences that are cleaved upon import into the organelle. The essential mitochondrial processing protease (MPP) is assumed to cleave the majority of incoming precursors; however, only a small fraction of mitochondrial precursors have been experi...

journal_title：Journal of proteome research

authors： Burkhart JM,Taskin AA,Zahedi RP,Vögtle FN

更新日期：2015-11-06 00:00:00

abstract：:Prostate cancer (PCa) is the second leading cause of cancer-related mortality in men. The prevalent diagnosis method is based on the serum prostate-specific antigen (PSA) screening test, which suffers from low specificity, overdiagnosis, and overtreatment. In this work, untargeted metabolomic profiling of age-matched ...

journal_title：Journal of proteome research

authors： Zang X,Jones CM,Long TQ,Monge ME,Zhou M,Walker LD,Mezencev R,Gray A,McDonald JF,Fernández FM

更新日期：2014-07-03 00:00:00

abstract：:We aimed to determine the time-course of metabolic changes related to the early onset of insulin resistance (IR), trying to evidence breaking points preceding the appearance of the clinical IR phenotype. The model chosen was the fructose (FRU)-fed rat compared to controls fed with starch. We focused on the hepatic met...

journal_title：Journal of proteome research

authors： Polakof S,Dardevet D,Lyan B,Mosoni L,Gatineau E,Martin JF,Pujos-Guillot E,Mazur A,Comte B

更新日期：2016-06-03 00:00:00