Qualitative and quantitative proteomic profiling of cripto(-/-) embryonic stem cells by means of accurate mass LC-MS analysis.

abstract：:The nation-wide electronic health record database acts as an interoperable repository of health data obtained throughout citizen contacts with health care providers. This system improves accessibility for citizens and researchers to health data with the ability to assign context to disease development. In that system,...

journal_title：Journal of proteome research

authors： Sepper R,Ross P,Tiik M

更新日期：2011-01-07 00:00:00

abstract：:The aim of current study was to investigate the metabolic changes associated with histidine supplementation in serum and urine metabolic signatures and serum amino acid (AA) profiles. Serum and urine 1H NMR-based metabolomics and serum AA profiles were employed in 32 and 37 obese women with metabolic syndrome (MetS) i...

journal_title：Journal of proteome research

authors： Du S,Sun S,Liu L,Zhang Q,Guo F,Li C,Feng R,Sun C

更新日期：2017-06-02 00:00:00

abstract：:An online nonenzymatic digestion method utilizing a microwave-heated flow cell and mild acid hydrolysis at aspartic acid (D) for rapid protein identification is described. This methodology, here termed microwave D-cleavage, was tested with proteins ranging in size from 5 kDa (insulin) to 67 kDa (bovine serum albumin) ...

journal_title：Journal of proteome research

authors： Hauser NJ,Basile F

更新日期：2008-03-01 00:00:00

abstract：:Wilson's Disease (WD), a copper transport disorder caused by a genetic defect in the ATP7B gene, has been a long time strong candidate for newborn screening (NBS), since early interventions can give better results by preventing irreversible neurological disability or liver cirrhosis. Several previous pilot studies mea...

journal_title：Journal of proteome research

authors： Jung S,Whiteaker JR,Zhao L,Yoo HW,Paulovich AG,Hahn SH

更新日期：2017-02-03 00:00:00

abstract：:An efficient means for the identification of prognostic and predictive biomarkers is essential in today's cancer management. A new approach toward biomarker discovery has therefore been proposed, where pathways instead of individual proteins would be monitored and targeted. Recently, the 'secretome', a biological flui...

journal_title：Journal of proteome research

authors： Lawlor K,Nazarian A,Lacomis L,Tempst P,Villanueva J

更新日期：2009-03-01 00:00:00

abstract：:As large-scale cross-linking data becomes available, new software tools for data processing and visualization are required to replace manual data analysis. XLink-DB serves as a data storage site and visualization tool for cross-linking results. XLink-DB accepts data generated with any cross-linker and stores them in a...

journal_title：Journal of proteome research

authors： Zheng C,Weisbrod CR,Chavez JD,Eng JK,Sharma V,Wu X,Bruce JE

更新日期：2013-04-05 00:00:00

abstract：:"Dry eye" is a multifactorial inflammatory disease affecting the ocular surface. Tear hyperosmolarity in dry eye contributes to inflammation and cell damage. Recent research efforts on dry eye have been directed toward biomarker discovery for diagnosis, response to treatment, and disease mechanisms. This study employe...

journal_title：Journal of proteome research

authors： Chen L,Li J,Guo T,Ghosh S,Koh SK,Tian D,Zhang L,Jia D,Beuerman RW,Aebersold R,Chan EC,Zhou L

更新日期：2015-09-04 00:00:00

abstract：:Genomic-scale analysis of protein function is currently limited by the ability to rapidly express the enormous diversity of protein targets in their active form. We describe a method to construct transcriptionally active expression templates (ETs) in parallel using a single PCR step wherein the overlap-extension react...

journal_title：Journal of proteome research

authors： Woodrow KA,Airen IO,Swartz JR

更新日期：2006-12-01 00:00:00

abstract：:Rapid early triage and dose estimation is vital for limited medical resource allocation and treatment of a large number of the wounded after radiological accidents. Lipidomics has been utilized to delineate biofluid lipid signatures after irradiation. Here, high-coverage targeted lipidomics was employed to screen radi...

journal_title：Journal of proteome research

authors： Xi C,Zhao H,Lu X,Cai TJ,Li S,Liu KH,Tian M,Liu QJ

更新日期：2021-01-01 00:00:00

abstract：:Plastids are essential organelles because they contribute to primary and secondary metabolism and plant signaling networks. A high-quality inventory of the plastid proteome is therefore a critical tool in plant research. We present reference plastid proteomes for maize (Zea mays) and Arabidopsis (Arabidopsis thaliana)...

journal_title：Journal of proteome research

authors： Huang M,Friso G,Nishimura K,Qu X,Olinares PD,Majeran W,Sun Q,van Wijk KJ

更新日期：2013-01-04 00:00:00

abstract：:Microglial inflammatory responses affect Parkinson's disease (PD) associated nigrostriatal degeneration. This is triggered, in measure, by misfolded, nitrated alpha-synuclein (N-alpha-syn) contained within Lewy bodies that are released from dying or dead dopaminergic neurons into the extravascular space. N-alpha-syn-s...

journal_title：Journal of proteome research

authors： Reynolds AD,Stone DK,Mosley RL,Gendelman HE

更新日期：2009-07-01 00:00:00

abstract：:In line with the aims of the Chromosome-based Human Proteome Project and the Biology/Disease-based Human Proteome Project, we have been studying differentially expressed transcripts and proteins in gliomas—the most prevalent primary brain tumors. Here, we present a perspective on important insights from this analysis ...

journal_title：Journal of proteome research

authors： Jayaram S,Gupta MK,Shivakumar BM,Ghatge M,Sharma A,Vangala RK,Sirdeshmukh R

更新日期：2015-09-04 00:00:00

abstract：:Nostoc punctiforme ATCC 29133 is a photoautotrophic cyanobacterium with the capacity to fix atmospheric N 2. Its ability to mediate this process is similar to that described for Nostoc sp. PCC 7120, where vegetative cells differentiate into heterocysts. Quantitative proteomic investigations at both the filament level ...

journal_title：Journal of proteome research

authors： Ow SY,Noirel J,Cardona T,Taton A,Lindblad P,Stensjö K,Wright PC

更新日期：2009-01-01 00:00:00

abstract：:Hepatocellular carcinoma (HCC) remains as the fifth most common cancer in the world and accounts for more than 700,000 deaths annually. Changes in serum glycosylation have long been associated with this cancer but the source of that material is unknown and direct glycan analysis of HCC tissues has been limited. Our la...

journal_title：Journal of proteome research

authors： West CA,Wang M,Herrera H,Liang H,Black A,Angel PM,Drake RR,Mehta AS

更新日期：2018-10-05 00:00:00

abstract：:Metaproteomics provides a direct measure of the functional information by investigating all proteins expressed by a microbiota. However, due to the complexity and heterogeneity of microbial communities, it is very hard to construct a sequence database suitable for a metaproteomic study. Using a public database, resear...

journal_title：Journal of proteome research

authors： Xiao J,Tanca A,Jia B,Yang R,Wang B,Zhang Y,Li J

更新日期：2018-04-06 00:00:00

abstract：:We have investigated whether postexercise ingestion of carbohydrates in combination with proteins generates a different systemic metabolic response, as compared to the sole ingestion of carbohydrate or water, in the early recovery phase following exercise. In addition, metabolic patterns related to fitness level were ...

journal_title：Journal of proteome research

authors： Chorell E,Moritz T,Branth S,Antti H,Svensson MB

更新日期：2009-06-01 00:00:00

abstract：:Cytological examination of cells from bronchoalveolar lavage (BAL) is commonly used for the diagnosis of lung cancer. Proteins released from lung cancer cells into BAL may serve as biomarkers for cancer detection. In this study, N-glycoproteins in eight cases of BAL fluid, as well as eight lung adenocarcinoma tissues ...

journal_title：Journal of proteome research

authors： Li QK,Shah P,Li Y,Aiyetan PO,Chen J,Yung R,Molena D,Gabrielson E,Askin F,Chan DW,Zhang H

更新日期：2013-08-02 00:00:00

abstract：:Yellow fever virus (YFV) replication is highly dependent on host cell factors. YFV NS4B is reported to be involved in viral replication and immune evasion. Here interactions between NS4B and human proteins were determined using a GST pull-down assay and analyzed using 1-DE and LC-MS/MS. We present a total of 207 prote...

journal_title：Journal of proteome research

authors： Vidotto A,Morais AT,Ribeiro MR,Pacca CC,Terzian AC,Gil LH,Mohana-Borges R,Gallay P,Nogueira ML

更新日期：2017-04-07 00:00:00

abstract：:We report the use of chemical derivatization with MALDI-MS/MS analysis for de novo sequence analysis. Using three frequently used homology-based search algorithms, we were able to identify more than 40 proteins from banana, a nonmodel plant with unsequenced genome. Furthermore, this approach allowed the identification...

journal_title：Journal of proteome research

authors： Samyn B,Sergeant K,Carpentier S,Debyser G,Panis B,Swennen R,Van Beeumen J

更新日期：2007-01-01 00:00:00

abstract：:Various mammalian cells including tumor cells secrete extracellular vesicles (EVs), otherwise known as exosomes and microvesicles. EVs are nanosized bilayered proteolipids and play multiple roles in intercellular communication. Although many vesicular proteins have been identified, their functional interrelationships ...

journal_title：Journal of proteome research

authors： Choi DS,Yang JS,Choi EJ,Jang SC,Park S,Kim OY,Hwang D,Kim KP,Kim YK,Kim S,Gho YS

更新日期：2012-02-03 00:00:00

abstract：:Early pathogenic events in Alzheimer's disease (AD) involve increased production and/or reduced clearance of beta-amyloid (Abeta), especially the 42 amino acid fragment Abeta1-42. The Abeta1-42 peptide is generated through cleavage of the amyloid precursor protein by beta- and gamma-secretase and is catabolised by a v...

journal_title：Journal of proteome research

authors： Portelius E,Westman-Brinkmalm A,Zetterberg H,Blennow K

更新日期：2006-04-01 00:00:00

abstract：:The plant cell wall is a dynamic cellular compartment consisting of a complex matrix of components that can change dramatically in response to environmental stresses. During pathogen attack, for instance, a wide spectrum of proteins that participate in various sequential processes involved in plant defense is secreted...

journal_title：Journal of proteome research

authors： Cheng FY,Blackburn K,Lin YM,Goshe MB,Williamson JD

更新日期：2009-01-01 00:00:00

abstract：:Accurate predictions of peptide retention times (RT) in liquid chromatography have many applications in mass spectrometry-based proteomics. Most notably such predictions are used to weed out incorrect peptide-spectrum matches, and to design targeted proteomics experiments. In this study, we describe a RT predictor, EL...

journal_title：Journal of proteome research

authors： Moruz L,Tomazela D,Käll L

更新日期：2010-10-01 00:00:00

abstract：:Clinical diagnostics and biomarker discovery are the major focuses of current clinical proteomics. In the present study, we applied microfluidic technology on a chip for proteome profiling of human urine from 31 normal healthy individuals (15 males and 16 females), 6 patients with diabetic nephropathy (DN), and 4 pati...

journal_title：Journal of proteome research

authors： Thongboonkerd V,Songtawee N,Sritippayawan S

更新日期：2007-05-01 00:00:00

abstract：:The Baculoviral IAP repeat-containing protein 5 (BIRC5), also known as inhibitor of apoptosis protein survivin, is a member of the chromosomal passenger complex and a key player in mitosis. To investigate the function of BIRC5 in liver regeneration, we analyzed a hepatocyte-specific BIRC5-knockout mouse model using a ...

journal_title：Journal of proteome research

authors： Bracht T,Hagemann S,Loscha M,Megger DA,Padden J,Eisenacher M,Kuhlmann K,Meyer HE,Baba HA,Sitek B

更新日期：2014-06-06 00:00:00

abstract：:Liver is unique in its capability to regenerate after an injury. Liver regeneration after a 2/3 partial hepatectomy served as a classical model and is adopted frequently to study the mechanism of liver regeneration. In the present study, semiquantitative analysis of protein expression in mouse liver regeneration follo...

journal_title：Journal of proteome research

authors： Hsieh HC,Chen YT,Li JM,Chou TY,Chang MF,Huang SC,Tseng TL,Liu CC,Chen SF

更新日期：2009-02-01 00:00:00

abstract：:The increasing occurrence of multidrug resistant tuberculosis exerts a major burden on treatment of this infectious disease. Thioridazine, previously used as a neuroleptic, is active against extensively drug resistant tuberculosis when added to other second- and third-line antibiotics. By quantitatively studying the p...

journal_title：Journal of proteome research

authors： de Keijzer J,Mulder A,de Haas PE,de Ru AH,Heerkens EM,Amaral L,van Soolingen D,van Veelen PA

更新日期：2016-06-03 00:00:00

abstract：:Protein post-translational modification by ubiquitin-fold modifier 1, UFM1, regulates many biological processes such as response to endoplasmic reticulum stress and regulation of tumor progression. A recent study has indicated that the UFM1-binding and PCI domain-containing protein 1 (UFBP1) is required for the conjug...

journal_title：Journal of proteome research

authors： Zhu Y,Lei Q,Li D,Zhang Y,Jiang X,Hu Z,Xu G

更新日期：2018-04-06 00:00:00

abstract：:Ribosomal proteins of the model gram-positive bacterium B. subtilis 168 were extensively characterized in a proteomic study. Mass spectra of the 52 proteins expected to be constitutive components of the 70S ribosome were recorded. Peptide MS/MS analysis with an average sequence coverage of 85% supported the identifica...

journal_title：Journal of proteome research

authors： Lauber MA,Running WE,Reilly JP

更新日期：2009-09-01 00:00:00

abstract：:Measuring and evaluating diagnostic efficiency is important in biomarker discovery and validation. The receiver operating characteristic (ROC) curve is a graphical plot for assessing the performance of a classifier or predictor that can be used to test the sensitivity and specificity of diagnostic biomarkers. In this ...

journal_title：Journal of proteome research

authors： Jeong SK,Na K,Kim KY,Kim H,Paik YK

更新日期：2012-12-07 00:00:00