Abstract：
:Protein biomarker discovery in the low concentration range of human body fluids requires the enrichment of the proteins of interest. Here we report on a tandem affinity strategy: In the first step, we isolated a human plasma glyco-subproteome of healthy individuals by wheat germ agglutinin (WGA) lectin affinity chromatography. In the second step, the proteins of this subproteome were used to raise antibodies in llama (Lama glama). The heavy-chain fraction of the llama antibodies was used to deplete from the WGA lectin binding fraction all proteins normally found in human plasma. In this way, we selectively enriched the glycoprotein, CEA, a known cancer marker which had been spiked into normal plasma. As a proof of concept, we applied this method to the analysis of plasma sample from colon cancer patients. We could demonstrate the selective enrichment of CEA by a factor of 600-800.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Mortezai N,Harder S,Schnabel C,Moors E,Gauly M,Schlüter H,Wagener C,Buck Fdoi
10.1021/pr100224ysubject
Has Abstractpub_date
2010-12-03 00:00:00pages
6126-34issue
12eissn
1535-3893issn
1535-3907journal_volume
9pub_type
杂志文章abstract：:Papillary thyroid carcinoma (PTC) is the most common type of thyroid cancer among women worldwide. It is confirmed mainly by fine-needle aspiration biopsy (FNAB), an invasive diagnostic method. The key proteins responsible for thyroid hormone biosynthesis are glycosylated. Hence, changes in site-specific glycosylation...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.9b00672
更新日期：2020-07-02 00:00:00
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journal_title：Journal of proteome research
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更新日期：2009-03-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2019-03-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2017-02-03 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr800423x
更新日期：2008-12-01 00:00:00
abstract：:Campylobacter jejuni is the most common cause of bacterial gastroenteritis in the developed world. Immunoproteomics highlighted a 42-45 kDa antigen that comigrated on two-dimensional (2-DE) gels with the C. jejuni major outer membrane protein (MOMP). Predictive analysis revealed two candidates for the identity of the ...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr900544x
更新日期：2009-10-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr400738a
更新日期：2013-12-06 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.7b00428
更新日期：2018-01-05 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr200039p
更新日期：2011-07-01 00:00:00
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pub_type： 杂志文章
doi：10.1021/pr300389r
更新日期：2012-11-02 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2019-04-05 00:00:00
abstract：:Plastids are essential organelles because they contribute to primary and secondary metabolism and plant signaling networks. A high-quality inventory of the plastid proteome is therefore a critical tool in plant research. We present reference plastid proteomes for maize (Zea mays) and Arabidopsis (Arabidopsis thaliana)...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr300952g
更新日期：2013-01-04 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr300280t
更新日期：2012-06-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr1011119
更新日期：2011-04-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2005-03-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr049916l
更新日期：2004-09-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr800420s
更新日期：2008-10-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr400435d
更新日期：2013-09-06 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.5b01031
更新日期：2016-07-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2011-06-03 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2020-09-04 00:00:00
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更新日期：2007-11-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2007-05-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.6b00144
更新日期：2017-02-03 00:00:00
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journal_title：Journal of proteome research
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更新日期：2003-09-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr401267m
更新日期：2014-06-06 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr4010597
更新日期：2014-02-07 00:00:00
abstract：:Phosphorylation is a post-translational modification with a vital role in cellular signaling. Isobaric labeling-based strategies, such as tandem mass tags (TMT), can measure the relative phosphorylation states of peptides in a multiplexed format. However, the low stoichiometry of protein phosphorylation constrains the...
journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2020-01-03 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2021-01-01 00:00:00
abstract：:MSI is a molecular imaging technique that allows for the generation of topographic 2D maps for various endogenous and some exogenous molecules without prior specification of the molecule. In this paper, we start with the premise that a region of interest (ROI) is given to us based on preselected morphological criteria...
journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr101159e
更新日期：2011-04-01 00:00:00
