Abstract:
:Post-translational nitration of proteins was analyzed by capillary reversed-phase high-performance liquid chromatography (RP-HPLC) on-line interfaced to electrospray ionization mass spectrometry (ESI--MS) or tandem mass spectrometry (ESI--MS/MS). Both methods were compared using a tryptic digest of bovine serum albumin (BSA) and yielded sequence coverages of 95% and 33% with RP-HPLC--ESI--MS and RP-HPLC--ESI--MS/MS, respectively. At least 95% of the tyrosines were covered by the former method, whereas the latter method only detected less than 50% of the tyrosine-containing peptides. Upon liquid-phase nitration of BSA in aqueous solution using an excess of tetranitromethane, at least 16 of the 20 tyrosine residues were found to be nitrated. After exposure of solid BSA samples to gaseous nitrogen dioxide and ozone at atmospherically relevant concentration levels, only 3 nitrated peptides were detected. By use of such a model system, RP-HPLC--ESI--MS proved to be a rapid and highly efficient method for the comprehensive and quantitative detection of protein nitration.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Walcher W,Franze T,Weller MG,Pöschl U,Huber CGdoi
10.1021/pr034034skeywords:
subject
Has Abstractpub_date
2003-09-01 00:00:00pages
534-42issue
5eissn
1535-3893issn
1535-3907journal_volume
2pub_type
杂志文章abstract::Fusarium graminearum (FG) is a serious plant pathogen causing huge losses in global production of wheat and other cereals. Tri5-gene encoded trichodiene synthase is the first key enzyme for biosynthesis of trichothecene mycotoxins in FG. To further our understandings of FG metabolism which is essential for developing ...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr101095t
更新日期:2011-05-06 00:00:00
abstract::Ovarian cancer remains a deadly threat to women as the disease is often diagnosed in the late stages when the chance of survival is low. There are no good biomarkers available for early detection and only a few markers have shown clinical utility for prognosis, response to therapy and disease recurrence. We mined cond...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr900411g
更新日期:2009-10-01 00:00:00
abstract::Human plasma and serum proteins are subject to intrinsic proteolytic degradation both during and after blood collection. By monitoring peptides, we investigated the stability of plasma and serum samples and the effects of anticoagulants and protease inhibitors on the plasma samples. Serum and plasma were subjected to ...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr060550h
更新日期:2007-05-01 00:00:00
abstract::Francisella tularensis causes disease (tularemia) in a large number of mammals, including man. We previously demonstrated enhanced efficacy of conventional antibiotic therapy for tularemia by postexposure passive transfer of immune sera developed against a F. tularensis LVS membrane protein fraction (MPF). However, th...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr500628k
更新日期:2015-02-06 00:00:00
abstract::During vertebrate central nervous system development, the apical neuroepithelium is bathed with embryonic Cerebrospinal Fluid (e-CSF) which plays regulatory roles in cortical cell proliferation and maintenance. Here, we report the first proteomic analysis of human e-CSF and compare it to an extensive proteomic analysi...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr070247w
更新日期:2007-09-01 00:00:00
abstract::Peptide abundance estimation is generally the first step in protein quantification. In peptide abundance estimation, peptide overlapping and peak intensity variation are two challenges. The main objective of this paper is to estimate peptide abundance by taking advantage of peptide isotopic distribution and smoothness...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr100181g
更新日期:2010-05-07 00:00:00
abstract::A rate-limiting and costly step in many proteomics analyses is the cloning of all of the ORFs for an organism into technique-specific vectors. Here, we describe the generation of a Campylobacter jejuni expression clone set using a high-throughput cloning approach based on recombination in E. coli. The approach uses na...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr0341134
更新日期:2004-05-01 00:00:00
abstract::Nutrient starvation is an important survival challenge for bacteria during industrial production of functional foods. As next-generation sequencing technology has greatly advanced, we performed proteomic and genomic analysis to investigate the response of Lactobacillus casei Zhang to a glucose-restricted environment. ...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.7b00886
更新日期:2018-03-02 00:00:00
abstract::The urine metabotype of 12 individuals was followed over a period of 8-10 years, which provided the longest longitudinal study of metabolic phenotypes to date. More than 2000 NMR metabolic profiles were analyzed. The majority of subjects have a stable metabotype. Subjects who were exposed to important pathophysiologic...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.5b00275
更新日期:2015-07-02 00:00:00
abstract::In systemic amyloidosis, accumulation of misfolded proteins as extracellular amyloid fibrils in tissues causes severe organ dysfunction, but the molecular events of tissue damage related to amyloid deposition are still largely unknown. Through the use of the MudPIT proteomic approach, comprehensive protein profiles of...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr400583h
更新日期:2013-12-06 00:00:00
abstract::Vascular leakage is a hallmark of severe dengue infection. Although extensive studies have been conducted during the past several decades, the molecular mechanisms underlying vascular leakage in dengue shock syndrome (DSS) remain unclear. We thus performed a proteomics study to characterize responses in human endothel...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr900060g
更新日期:2009-05-01 00:00:00
abstract::Ribosomal proteins of the model gram-positive bacterium B. subtilis 168 were extensively characterized in a proteomic study. Mass spectra of the 52 proteins expected to be constitutive components of the 70S ribosome were recorded. Peptide MS/MS analysis with an average sequence coverage of 85% supported the identifica...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr801114k
更新日期:2009-09-01 00:00:00
abstract::While the proteome defines the expressed gene products, the metabolome results from reactions controlled by such gene products. Plasma represents an accessible "window" to the metabolome both in regard of availability and content. The wide range of the plasma metabolome, in terms of molecular diversity and abundance, ...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr8001639
更新日期:2008-08-01 00:00:00
abstract::Development of early embryonic stages before activation of the embryonic genome depends on sufficiently stored products of the maternal genome, adequate recruitment and degradation of mRNAs, as well as activation, deactivation, and relocation of proteins. By application of an isobaric tagging for relative and absolute...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr500550t
更新日期:2014-10-03 00:00:00
abstract::Mass spectrometry (MS) is a widely used proteome analysis tool for biomedical science. In an MS-based bottom-up proteomic approach to protein identification, sequence database (DB) searching has been routinely used because of its simplicity and convenience. However, searching a sequence DB with multiple variable modif...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.6b01019
更新日期:2017-12-01 00:00:00
abstract::Sulfur trafficking systems are multiprotein systems that synthesize sulfur-containing cofactors such as iron-sulfur clusters. The sulfur is derived enzymatically from cysteine and transferred between nucleophilic cysteine residues within proteins until incorporation into the relevant cofactor. As these systems are poo...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr100920r
更新日期:2010-12-03 00:00:00
abstract::Hepatocellular carcinoma (HCC) causes more than half a million annual deaths worldwide. Understanding the mechanisms contributing to HCC development is highly desirable for improved surveillance, diagnosis, and treatment. Liver tissue metabolomics has the potential to reflect the physiological changes behind HCC devel...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.9b00185
更新日期:2019-08-02 00:00:00
abstract::The past decade has been marked by the emergence of selective affinity media and sensitive mass spectrometry instrumentation that facilitated large-scale phosphoproteome analyses and expanded the repertoire of protein phosphorylation. Despite these remarkable advances, the precise location of the phosphorylation site ...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr300229m
更新日期:2012-07-06 00:00:00
abstract::Protein interaction networks display approximate scale-free topology, in which hub proteins that interact with a large number of other proteins determine the overall organization of the network. In this study, we aim to determine whether hubs are distinguishable from other networked proteins by specific sequence featu...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr060171o
更新日期:2006-11-01 00:00:00
abstract::Geobacter sulfurreducens is a dissimilatory metal-reducing bacterium capable of forming thick electron-conducting biofilms on solid electrodes. Here, we employ for the first time comparative proteomics to identify key physiological changes involved in G. sulfurreducens adaptation from fumarate-respiring planktonic cel...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.5b01019
更新日期:2016-01-01 00:00:00
abstract::As work on systematic searches for developmentally-regulated proteins is limited, whole brains of CL57B/6J mice were taken from day 16 of gestation, at postnatal days 7 and 54-58. Proteins were extracted, and analyzed by a gel-based proteomic method (ion trap HCT). Antioxidant, cytoskeleton, chaperone, several metabol...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr800990x
更新日期:2009-03-01 00:00:00
abstract::Protein phosphorylation is a globally adopted and tightly controlled post-translational modification, and represents one of the most important molecular switching mechanisms that govern the entire spectrum of biological processes. In the central nervous system, it has been demonstrated that phosphorylation of key prot...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr8003198
更新日期:2008-11-01 00:00:00
abstract::Tumor invasion defines the transition between tissue-restricted carcinomas, related to good outcome as optimal surgery becomes possible, and metastatic tumors associated with poor prognosis and a dramatic decrease in survival. In endometrial cancer, myometrial infiltration represents a determinant parameter highly val...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr900390t
更新日期:2009-10-01 00:00:00
abstract::Candida albicans is a commensal microorganism in the oral cavity and gastrointestinal and urogenital tracts of most individuals that acts as an opportunistic pathogen when the host immune response is reduced. Here, we established different immunocompetent murine models to analyze the antibody responses to the C. albic...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.6b00383
更新日期:2017-01-06 00:00:00
abstract::Growing appreciation for astrocytes as active participants in nervous system development, neurovascular metabolic coupling, and neurological disease progression has stimulated recent investigation into specific astrocyte-secreted proteins that may mediate these functions. The current work utilized SILAC-generated isot...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr100134n
更新日期:2010-05-07 00:00:00
abstract::By foliar fortification with selenite, selenium (Se)-enriched rice with a higher Se content and grain yield has been generated. However, the regulatory mechanisms of Se response in rice grains remain unknown; therefore, we carried out a comparative proteomics study in Se-enriched rice grains by using two approaches in...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr300878y
更新日期:2013-02-01 00:00:00
abstract::Ubiquitin ligases (E3s) serve as key regulators for the ubiquitylation-mediated pathway. The identification of the corresponding relationship between E3 and its substrates is challenging but required for understanding the regulatory network of ubiquitylation. The low abundance of ubiquitinated conjugates and high redu...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.9b00673
更新日期:2020-01-03 00:00:00
abstract::Royal jelly (RJ) is an exclusive food for queen honey bee (Apis mellifera L.) that is synthesized and secreted by young worker bees. RJ is also widely used in medical products, cosmetics, and as health foods. However, little is known about RJ functionality and the total protein components, although recent research is ...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr800061j
更新日期:2008-08-01 00:00:00
abstract::The present study reports an in-depth proteome analysis of two Lactobacillus rhamnosus strains, the well-known probiotic strain GG and the dairy strain Lc705. We used GeLC-MS/MS, in which proteins are separated using 1-DE and identified using nanoLC-MS/MS, to generate high-quality protein catalogs. To maximize the num...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr2000896
更新日期:2011-08-05 00:00:00
abstract::Pancreatic cancer is a formidable disease and early detection biomarkers are needed to make inroads into improving the outcomes in these patients. In this work, lectin antibody microarrays were utilized to detect unique glycosylation patterns of proteins from serum. Antibodies to four potential glycoprotein markers th...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr8007013
更新日期:2009-02-01 00:00:00