High-throughput cloning of Campylobacter jejuni ORfs by in vivo recombination in Escherichia coli.

abstract：:Human saliva is a protein-rich, easily accessible source of potential local and systemic biomarkers to monitor changes that occur under pathological conditions; however, little is known about the changes in abundance associated with normal aging. In this study, we performed a comprehensive proteomic profiling of poole...

journal_title：Journal of proteome research

authors： Ambatipudi KS,Lu B,Hagen FK,Melvin JE,Yates JR

更新日期：2009-11-01 00:00:00

abstract：:Histones regulate a variety of chromatin templated events by their post-translational modifications (PTMs). Although there are extensive reports on the PTMs of canonical histones, the information on the histone variants remains very scanty. Here, we report the identification of different PTMs, such as acetylation, met...

journal_title：Journal of proteome research

authors： Pentakota SK,Sandhya S,P Sikarwar A,Chandra N,Satyanarayana Rao MR

更新日期：2014-12-05 00:00:00

abstract：:Microorganisms release effector molecules that modulate the host machinery enabling survival, replication, and dissemination of a pathogen. Here we characterized the extracellular proteome of Paracoccidioides brasiliensis at its pathogenic yeast phase. Cell-free culture supernatants from the Pb18 isolate, cultivated i...

journal_title：Journal of proteome research

authors： Vallejo MC,Nakayasu ES,Matsuo AL,Sobreira TJ,Longo LV,Ganiko L,Almeida IC,Puccia R

更新日期：2012-03-02 00:00:00

abstract：:T lymphocytes mediate cellular and humoral defense against foreign bodies or autoantigens. An understanding of T-cell information processing furthers studies of the immunological response. We describe a large-scale phosphorylation analysis of primary T cells using a multidimensional separation strategy, involving prep...

journal_title：Journal of proteome research

authors： Carrascal M,Ovelleiro D,Casas V,Gay M,Abian J

更新日期：2008-12-01 00:00:00

abstract：:Novel biomarker verification assays are urgently required to improve the efficiency of biomarker development. Benefitting from lower development costs, multiple reaction monitoring (MRM) has been used for biomarker verification as an alternative to immunoassay. However, in general MRM analysis, only one sample can be ...

journal_title：Journal of proteome research

authors： Yin HR,Zhang L,Xie LQ,Huang LY,Xu Y,Cai SJ,Yang PY,Lu HJ

更新日期：2013-09-06 00:00:00

abstract：:We analyzed a unique rice (Oryza sativa L.) blast lesion mimic (blm) mutant for differentially expressed proteins in leaves of one- and two-week-old seedlings manifesting the lesion mimic phenotype. Gel-based one- and two-dimensional electrophoresis (1- and 2-DGE) was performed using leaves (blm and wild-type, WT) bef...

journal_title：Journal of proteome research

authors： Jung YH,Rakwal R,Agrawal GK,Shibato J,Kim JA,Lee MO,Choi PK,Jung SH,Kim SH,Koh HJ,Yonekura M,Iwahashi H,Jwa NS

更新日期：2006-10-01 00:00:00

abstract：:Autosomal dominant polycystic liver disease (PCLD) is characterized by multiple liver cysts and is caused by mutations in PRKCSH (hepatocystin). Mechanisms of cystogenesis are unknown, but previous studies have shown that hepatocystin is secreted in vitro. The goal of this study was to determine the fate of hepatocyst...

journal_title：Journal of proteome research

authors： Waanders E,Lameris AL,Op den Camp HJ,Pluk W,Gloerich J,Strijk SP,Drenth JP

更新日期：2008-06-01 00:00:00

abstract：:NMR ligand affinity screening is a powerful technique that is routinely used in drug discovery or functional genomics to directly detect protein-ligand binding events. Binding events can be identified by monitoring differences in the 1D (1)H NMR spectrum of a compound with and without protein. Although a single NMR sp...

journal_title：Journal of proteome research

authors： Stark JL,Eghbalnia HR,Lee W,Westler WM,Markley JL

更新日期：2016-04-01 00:00:00

abstract：:The paper describes the implementation of a software system based on the Fenyö disulfide bond assignment algorithm. The system allows an investigator to enter data derived from mass spectrum peak assignments, a target protein sequence and other experimental conditions. The output of the system is the set of disulfide ...

journal_title：Journal of proteome research

authors： Craig R,Krokhin O,Wilkins J,Beavis RC

更新日期：2003-11-01 00:00:00

abstract：:We have obtained the first direct electrochemistry of full-length inducible nitric oxide synthase (iNOS) by entrapping the enzyme in polyethylenimine (PEI) film. The interaction between iNOS and calmodulin (CaM) was then studied, which revealed an enhanced electron-transfer reactivity of the enzyme facilitated by CaM....

journal_title：Journal of proteome research

authors： Xiao H,Zhou H,Chen G,Liu S,Li G

更新日期：2007-04-01 00:00:00

abstract：:The world is currently facing the COVID-19 pandemic caused by the SARS-CoV-2 virus. The pandemic is causing the death of people around the world, and public and social health measures to slow or prevent the spread of COVID-19 are being implemented with the involvement of all members of society. Research institutions a...

journal_title：Journal of proteome research

authors： Barros RO,Junior FLCC,Pereira WS,Oliveira NMN,Ramos RM

更新日期：2020-11-06 00:00:00

abstract：:Ovarian granulosa cell tumors (GCTs) are hormonally active cancers characterized by indolent growth and late, invasive relapse. No therapies have yet proven to be efficacious. We previously reported that the inhibition of the antiapoptotic X-linked inhibitor of apoptosis protein (XIAP) removes transrepression of the p...

journal_title：Journal of proteome research

authors： Leung DTH,Rainczuk A,Nguyen T,Stephens A,Silke J,Fuller PJ,Chu S

更新日期：2019-04-05 00:00:00

abstract：:By foliar fortification with selenite, selenium (Se)-enriched rice with a higher Se content and grain yield has been generated. However, the regulatory mechanisms of Se response in rice grains remain unknown; therefore, we carried out a comparative proteomics study in Se-enriched rice grains by using two approaches in...

journal_title：Journal of proteome research

authors： Wang YD,Wang X,Ngai SM,Wong YS

更新日期：2013-02-01 00:00:00

abstract：:One application of 2D gel electrophoresis is to reveal differences in protein pattern between two or more groups of individuals, attributable to their group membership. Multivariate data analytical methods are useful in pinpointing the spots relevant for discrimination by focusing not only on single spot differences, ...

journal_title：Journal of proteome research

authors： Jensen KN,Jessen F,Jørgensen BM

更新日期：2008-03-01 00:00:00

abstract：:Recent studies have shown a remarkable degree of plasticity in the N-glycome of the model nematode Caenorhabditis elegans; ablation of glycosylation-relevant genes can result in radically altered N-glycan profiles despite only minor biological phenotypic effects. Up to four fucose residues and five different linkages ...

journal_title：Journal of proteome research

authors： Yan S,Jin C,Wilson IB,Paschinger K

更新日期：2015-12-04 00:00:00

abstract：:Stroke remains a major public health problem worldwide; it causes severe disability and is associated with high mortality rates. However, early diagnosis of stroke is difficult, and no reliable biomarkers are currently established. In this study, mass-spectrometry-based metabolomics was utilized to characterize the me...

journal_title：Journal of proteome research

authors： Liu P,Li R,Antonov AA,Wang L,Li W,Hua Y,Guo H,Wang L,Liu P,Chen L,Tian Y,Xu F,Zhang Z,Zhu Y,Huang Y

更新日期：2017-02-03 00:00:00

abstract：:Due to the increasing prevalence of nosocomial and community-acquired antibiotic resistant Staphylococcus aureus (SA), understanding the determinants of SA nasal carriage has become a major imperative. Previous research has revealed many host and bacterial factors that contribute to SA nasal carriage. To assess bacter...

journal_title：Journal of proteome research

authors： Muthukrishnan G,Quinn GA,Lamers RP,Diaz C,Cole AL,Chen S,Cole AM

更新日期：2011-04-01 00:00:00

abstract：:The mechanisms whereby Mycobacterium tuberculosis (Mtb) rewires the host metabolism in vivo are surprisingly unexplored. Here, we used three high-resolution mass spectrometry platforms to track altered lung metabolic changes associated with Mtb infection of mice. The multiplatform data sets were merged using consensus...

journal_title：Journal of proteome research

authors： Fernández-García M,Rey-Stolle F,Boccard J,Reddy VP,García A,Cumming BM,Steyn AJC,Rudaz S,Barbas C

更新日期：2020-05-01 00:00:00

abstract：:There is a legion of hypothetical proteins (HP) in prokaryotic and eukaryotic proteomes and the aim of this study was to describe HP in the perireticular nucleus (PN), a key structure in human brain development. Tissue from four PNs was homogenized and extracted proteins were run on two-dimensional gel electrophoresis...

journal_title：Journal of proteome research

authors： Hepner F,Myung JK,Ulfig N,Pollak A,Lubec G

更新日期：2005-11-01 00:00:00

abstract：:Ureteral obstruction will lead clinically to hydronephrosis, which may further develop into partial or complete loss of kidney function and even cause permanent histological damage. However, there is little knowledge of metabolic responses during the obstructed process and its recoverability. In this study, a complete...

journal_title：Journal of proteome research

authors： Wang Z,Xu R,Shen G,Feng J

更新日期：2018-09-07 00:00:00

abstract：:Ubiquitin ligases (E3s) serve as key regulators for the ubiquitylation-mediated pathway. The identification of the corresponding relationship between E3 and its substrates is challenging but required for understanding the regulatory network of ubiquitylation. The low abundance of ubiquitinated conjugates and high redu...

journal_title：Journal of proteome research

authors： Lan Q,Wang Y,Sun Z,Li Y,Zhang C,Chang L,Gao Y,Wu J,Wang F,Xu P

更新日期：2020-01-03 00:00:00

abstract：:Huntington's disease (HD) causes widespread CNS changes and systemic abnormalities including endocrine and immune dysfunction. HD biomarkers are needed to power clinical trials of potential treatments. We used multiplatform proteomic profiling to reveal plasma changes with HD progression. Proteins of interest were eva...

journal_title：Journal of proteome research

authors： Dalrymple A,Wild EJ,Joubert R,Sathasivam K,Björkqvist M,Petersén A,Jackson GS,Isaacs JD,Kristiansen M,Bates GP,Leavitt BR,Keir G,Ward M,Tabrizi SJ

更新日期：2007-07-01 00:00:00

abstract：:The Mycobacterium tuberculosis membrane is rich in antigens that are potential targets for diagnostics and the development of new vaccines. To better understand the mechanisms underlying MTB virulence and identify new targets for therapeutic intervention, we investigated the differential composition of membrane proteo...

journal_title：Journal of proteome research

authors： Gunawardena HP,Feltcher ME,Wrobel JA,Gu S,Braunstein M,Chen X

更新日期：2013-12-06 00:00:00

abstract：:Translationally controlled tumor protein (TCTP) is a highly conserved housekeeping protein present in eukaryotic organisms. It is involved in regulating many fundamental processes and plays a critical role in tumor reversion and tumorigenesis. Increasing evidence suggests that TCTP plays a role in the regulation of ce...

journal_title：Journal of proteome research

authors： Li S,Chen M,Xiong Q,Zhang J,Cui Z,Ge F

更新日期：2016-10-07 00:00:00

abstract：:Human milk oligosaccharides (HMOs) perform a number of functions including serving as prebiotics to stimulate the growth of beneficial intestinal bacteria, as receptor analogues to inhibit binding of pathogens, and as substances that promote postnatal brain development. There is further evidence that HMOs participate ...

journal_title：Journal of proteome research

authors： Wu S,Tao N,German JB,Grimm R,Lebrilla CB

更新日期：2010-08-06 00:00:00

abstract：:De Palma et al. published a research paper in which they describe the effect of chronic hypoxia on rat skeletal muscle metabolism by means of a comparative proteomic analysis (J. Proteome Res. 2007, 6(5), 1974-1984). For this, relatively young animals were used. In our communication, we note that, based on other liter...

journal_title：Journal of proteome research

authors： Gosker HR,Schols AM

更新日期：2007-09-01 00:00:00

abstract：:A novel linear ion trap (LIT) mass spectrometer with dual matrix-assisted laser desorption/ionization (MALDI) and electrospray ionization (ESI) ionization sources has been built in the MALDI-LIT-ESI configuration. The design features two independent ion source/ion optical channels connected to opposite ends of a singl...

journal_title：Journal of proteome research

authors： Smith SA,Blake TA,Ifa DR,Cooks RG,Ouyang Z

更新日期：2007-02-01 00:00:00

abstract：:Hematopoietic stem cell transplantation is the oldest and most successful form of stem cell therapy. High dose therapy (HDT) followed by hematopoietic stem cell transplantation allows physicians to administer increased amounts of chemotherapy and/or radiation while minimizing negative side effects such as damage to bl...

journal_title：Journal of proteome research

authors： Cano KE,Li L,Bhatia S,Bhatia R,Forman SJ,Chen Y

更新日期：2011-06-03 00:00:00

abstract：:Epithelial cells play an important role in physiological and pathophysiological situations, with organ-, tissue-, type-, and function-specific patterns. Proteome analysis has been used to study epithelial-origin diseases and identify novel prognostic, diagnostic, and therapeutic markers. The present review compares th...

journal_title：Journal of proteome research

authors： Zhao H,Adler KB,Bai C,Tang F,Wang X

更新日期：2006-04-01 00:00:00

abstract：:Neuroblastoma (NB) is an infant tumor which frequently differentiates into neurons. We used two-dimensional differential in-gel electrophoresis (2D-DIGE) to analyze the cytosolic and nuclear protein expression patterns of LAN-5 cells following neuronal differentiating agent all-trans-retinoic acid treatment. We identi...

journal_title：Journal of proteome research

authors： Cimmino F,Spano D,Capasso M,Zambrano N,Russo R,Zollo M,Iolascon A

更新日期：2007-07-01 00:00:00