Abstract:
:Epithelial cells play an important role in physiological and pathophysiological situations, with organ-, tissue-, type-, and function-specific patterns. Proteome analysis has been used to study epithelial-origin diseases and identify novel prognostic, diagnostic, and therapeutic markers. The present review compares the variation of sample preparation for epithelial proteomic analysis, search similarities, and differences of epithelial proteomics between different cells, locations, and diseases. We focus on specificity of proteomic markers for epithelial-involved diseases. Proteomic alterations in epithelial cell lines were mapped to understand protein patterns, differentiation, oncogenesis, and pathogenesis of epithelial-origin diseases. Changes of proteomic patterns depend on different epithelial cell lines, challenges, and preparation. Epithelial protein profiles associated with intracellular locations and protein function. Epithelial proteomics has been greatly developed to link clinical questions, e.g., disease severity, biomarkers for disease diagnosis, and drug targets. There is an exciting and attractive start to link epithelial proteomics with histology of clinical samples. From the present review, we can find that most of disease-associated investigation of epithelial proteomics has been focused on epithelial-origin cancer. There is a significant gap of epithelial proteomics between acute and chronic organ injury, inflammation, and multiple organ dysfunction. Epithelial proteomics will provide powerful information on the relationships between biological molecules and disease mechanisms. Epithelial proteomics strategies and approaches should become more global, multidimensional, and systemic.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Zhao H,Adler KB,Bai C,Tang F,Wang Xdoi
10.1021/pr050389vsubject
Has Abstractpub_date
2006-04-01 00:00:00pages
743-55issue
4eissn
1535-3893issn
1535-3907journal_volume
5pub_type
杂志文章,评审abstract::Complicating proteomic analysis of whole tissues is the obvious problem of cell heterogeneity in tissues, which often results in misleading or confusing molecular findings. Thus, the coupling of tissue microdissection for tumor cell enrichment with capillary isotachophoresis-based selective analyte concentration not o...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr300303t
更新日期:2012-08-03 00:00:00
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journal_title:Journal of proteome research
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journal_title:Journal of proteome research
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abstract::Sperm proteomes have emerged for several species; however, the extent of species similarity is unknown. Sheep are an important agricultural species for which a comprehensive sperm proteome has not been produced. In addition, potential proteomic factors from seminal plasma that may contribute to improved fertility afte...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.6b00530
更新日期:2016-10-07 00:00:00
abstract::A database of nonredundant structures of EF-hand domains--i.e., pairs of helix-loop-helix motifs--has been assembled, and the six angles among the four helices re-determined. A principal component analysis of these angles allows us to use two such components (PC1 and PC2) to describe the system retaining 80% of the to...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr050148n
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journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr200086v
更新日期:2011-08-05 00:00:00
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journal_title:Journal of proteome research
pub_type: 杂志文章
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更新日期:2008-08-01 00:00:00
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journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr034048y
更新日期:2004-01-01 00:00:00
abstract::Quantitative proteomics experiments, using for instance isobaric tandem mass tagging approaches, are conducive to measuring changes in protein abundance over multiple time points in response to one or more conditions or stimulations. The aim is often to determine which proteins exhibit similar patterns within and acro...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.8b00615
更新日期:2019-02-01 00:00:00
abstract::The production of virus-like particles (VLPs) has gained importance over the last few years owing to the benefits they provide compared to conventional vaccines. The biopharmaceutical industry is currently searching for safer candidates based on VLPs for new and existing vaccines and implementing new methods of manufa...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.9b00601
更新日期:2020-03-06 00:00:00
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journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr4008283
更新日期:2013-11-01 00:00:00
abstract::Fast (120 s) and high-throughput (more than six samples at once) in-gel trypsin digestion of proteins using sonoreactor technology has been achieved. Successful protein identification was done by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, MALDI-TOF-MS. Specific identification of the ...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr060508m
更新日期:2007-02-01 00:00:00
abstract::Cripto is one of the key regulators of embryonic stem cells (ESCs) differentiation into cardiomyocites vs neuronal fate. Cripto(-/-) murine ESCs have been utilized to investigate the molecular mechanisms underlying early events of mammalian lineage differentiation. 2D/LC-MS/MS and a label-free LC-MS approaches were us...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr800485c
更新日期:2009-02-01 00:00:00
abstract::Amyotrophic lateral sclerosis (ALS) is characterized by the absence of reliable diagnostic biomarkers. The aim of the study was to (i) devise an untargeted metabolomics methodology that reliably compares cerebrospinal fluid (CSF) from ALS patients and controls by liquid chromatography coupled to high-resolution mass s...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr400376e
更新日期:2013-08-02 00:00:00
abstract::N-glycosylation of proteins is well known to occur at asparagine residues that fall within the canonical consensus sequence N-X-S/T but has also been identified at a small number of asparagine residues within N-X-C motifs, including the N491 residue of human serotransferrin. Here we report novel glycosylation sites wi...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.5b00733
更新日期:2016-07-01 00:00:00
abstract::Equine grass sickness (EGS) is a frequently fatal disease of horses, responsible for the death of 1 to 2% of the U.K. horse population annually. The etiology of this disease is currently uncharacterized, although there is evidence it is associated with Clostridium botulinum neurotoxin in the gut. Prevention is current...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.7b00784
更新日期:2018-03-02 00:00:00
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journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr700596e
更新日期:2008-03-01 00:00:00
abstract::The SwePep database is designed for endogenous peptides and mass spectrometry. It contains information about the peptides such as mass, pl, precursor protein and potential post-translational modifications. Here, we have improved and extended the SwePep database with tandem mass spectra, by adding a locally curated ver...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr800036d
更新日期:2008-07-01 00:00:00
abstract::Middle-aged and old left ventricles (LVs) are structurally and functionally very similar. Compared to a young LV, both show increased wall thickness and increased cavity size, with preserved cardiac function. However, when a stressor such as myocardial infarction occurs, striking differences are revealed between young...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr700685e
更新日期:2008-02-01 00:00:00
abstract::We aimed to evaluate neuroendocrine pulmonary tumors (NEPT) by a novel method involving map tree construction by comparing all of the protein spots. We performed a proteomics analysis to assess the similarities in protein expression between neuroendocrine pulmonary tumors (NEPT), including typical carcinoids (TC), aty...
journal_title:Journal of proteome research
pub_type: 杂志文章
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更新日期:2006-03-01 00:00:00
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journal_title:Journal of proteome research
pub_type: 杂志文章
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journal_title:Journal of proteome research
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doi:10.1021/pr050137u
更新日期:2005-11-01 00:00:00
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更新日期:2015-11-06 00:00:00
abstract::High density lipoprotein (HDL) particles are blood-borne complexes whose plasma levels have been associated with protection from cardiovascular disease (CVD). Recent studies have demonstrated the existence of distinct HDL subspecies; however, these have been difficult to isolate and characterize biochemically. Here, w...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.5b00419
更新日期:2015-08-07 00:00:00
abstract::Human embryonic stem cells (hESCs) are promising in regenerative medicine (RM) due to their differentiation plasticity and proliferation potential. However, a major challenge in RM is the generation of a vascular system to support nutrient flow to newly synthesized tissues. Here we refined an existing method to genera...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.6b00180
更新日期:2016-06-03 00:00:00
abstract::Clostridium difficile in recent years has undergone rapid evolution and has emerged as a serious human pathogen. Proteomic approaches can improve the understanding of the diversity of this important pathogen, especially in comparing the adaptive ability of different C. difficile strains. In this study, TMT labeling an...
journal_title:Journal of proteome research
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更新日期:2013-03-01 00:00:00
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journal_title:Journal of proteome research
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更新日期:2017-02-03 00:00:00
abstract::The epidermal growth factor receptor (EGFR) is usually overexpressed in nasopharyngeal carcinoma (NPC) and is associated with pathogenesis of NPC. However, while EGFR-modulated intracellular proteins have been extensively studied, little is known concerning their extracellular counterparts. To identify EGFR-regulated ...
journal_title:Journal of proteome research
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pub_type: 杂志文章
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