当前位置： SCI文献检索 > JOURNAL OF PROTEOME RESEARCH期刊下所有文献 > Comprehensive analysis of conditioned media from ovarian cancer cell lines identifies novel candidate markers of epithelial ovarian cancer.

Comprehensive analysis of conditioned media from ovarian cancer cell lines identifies novel candidate markers of epithelial ovarian cancer.

Abstract：

:Ovarian cancer remains a deadly threat to women as the disease is often diagnosed in the late stages when the chance of survival is low. There are no good biomarkers available for early detection and only a few markers have shown clinical utility for prognosis, response to therapy and disease recurrence. We mined conditioned media of four ovarian cancer cell lines (HTB75, TOV-112D, TOV-21G and RMUG-S) by two-dimensional liquid chromatography-mass spectrometry. Each cell line represented one of the major histological types of epithelial ovarian cancer. We identified 2039 proteins from which 228 were extracellular and 192 were plasma membrane proteins. Within the latter list, we identified several known markers of ovarian cancer including three that are well established, namely, CA-125, HE4, and KLK6. The list of 420 extracellular and membrane proteins was cross-referenced with the proteome of ascites fluid to generate a shorter list of 51 potential biomarker candidates. According to Ingenuity Pathway Analysis, two of the top 10 diseases associated with the list of 51 proteins were cancer and reproductive diseases. We selected nine proteins for preliminary validation using 20 serum samples from healthy women and 10 from women with ovarian cancer. Of the nine proteins, clusterin (increase) and IGFBP6 (decrease) showed significant differences between women with or without ovarian cancer. We conclude that in-depth proteomic analysis of cell culture supernatants of ovarian cancer cell lines can identify potential ovarian cancer biomarkers that are worth further clinical validation.

journal_name

J Proteome Res

journal_title

Journal of proteome research

authors

Gunawardana CG,Kuk C,Smith CR,Batruch I,Soosaipillai A,Diamandis EP

doi

10.1021/pr900411g

subject

Has Abstract

pub_date

2009-10-01 00:00:00

pages

4705-13

issue

10

eissn

1535-3893

issn

1535-3907

journal_volume

8

pub_type

杂志文章

相关文献

JOURNAL OF PROTEOME RESEARCH文献大全
  • Statistical validation of peptide identifications in large-scale proteomics using the target-decoy database search strategy and flexible mixture modeling.

    abstract：:Reliable statistical validation of peptide and protein identifications is a top priority in large-scale mass spectrometry based proteomics. PeptideProphet is one of the computational tools commonly used for assessing the statistical confidence in peptide assignments to tandem mass spectra obtained using database searc...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr7006818

    authors： Choi H,Ghosh D,Nesvizhskii AI

    更新日期：2008-01-01 00:00:00

  • Ferritin heavy chain is the host factor responsible for HCV-induced inhibition of apoB-100 production and is required for efficient viral infection.

    abstract：:Hepatic fat export occurs by apolipoprotein B-100-containing lipoprotein production, whereas impaired production leads to liver steatosis. Hepatitis C virus (HCV) infection is associated to dysregulation of apoB-100 secretion and steatosis; however, the molecular mechanism by which HCV affects the apoB-100 secretion i...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr201128s

    authors： Mancone C,Montaldo C,Santangelo L,Di Giacomo C,Costa V,Amicone L,Ippolito G,Pucillo LP,Alonzi T,Tripodi M

    更新日期：2012-05-04 00:00:00

  • Red Blood Cell Metabolic Responses to Torpor and Arousal in the Hibernator Arctic Ground Squirrel.

    abstract：:Arctic ground squirrels provide a unique model to investigate metabolic responses to hibernation in mammals. During winter months these rodents are exposed to severe hypothermia, prolonged fasting, and hypoxemia. In the light of their role in oxygen transport/off-loading and owing to the absence of nuclei and organell...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/acs.jproteome.9b00018

    authors： Gehrke S,Rice S,Stefanoni D,Wilkerson RB,Nemkov T,Reisz JA,Hansen KC,Lucas A,Cabrales P,Drew K,D'Alessandro A

    更新日期：2019-04-05 00:00:00

  • Proteomic analysis of silk gland programmed cell death during metamorphosis of the silkworm Bombyx mori.

    abstract：:The silk gland of the silkworm Bombyx mori undergoes programmed cell death (PCD) during pupal metamorphosis. On the basis of their morphological changes and the occurrence of a DNA ladder, the tissue cells were categorized into three groups: intact, committed, and dying. To identify the proteins involved in this proce...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr070043f

    authors： Jia SH,Li MW,Zhou B,Liu WB,Zhang Y,Miao XX,Zeng R,Huang YP

    更新日期：2007-08-01 00:00:00

  • Antibiotic Exposure Disturbs the Gut Microbiota and Its Metabolic Phenotype Differently in Rats with Advanced-Stage Type 1 Diabetes and Age-Matched Controls.

    abstract：:Antibiotic-induced microbial perturbations alter host metabolism and affect host physiology. In this study, we aimed to investigate the effects of vancomycin (Vanc) and ciprofloxacin/metronidazole (CiMe) exposures on the gut microbiome and metabolome in the colonic content and tissue samples from advanced-stage type 1...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/acs.jproteome.9b00402

    authors： Zheng H,Fan K,Ji H,Jiang Q,Ning J,Xu P,Li C,Gao H

    更新日期：2019-11-01 00:00:00

  • Introduction of the disulfide proteome: application of a technique for the analysis of plant storage proteins as well as allergens.

    abstract：:Redox regulation plays an important role across a broad spectrum of biology. Accumulating evidence suggests that thioredoxin, a widely distributed redox enzyme, participates in the redox control of numerous target proteins, thus, playing a key role as a signaling intermediate that senses the metabolic state or environ...

    journal_title：Journal of proteome research

    pub_type： 杂志文章,评审

    doi：10.1021/pr8003453

    authors： Yano H,Kuroda S

    更新日期：2008-08-01 00:00:00

  • CREB Signaling Mediates Dose-Dependent Radiation Response in the Murine Hippocampus Two Years after Total Body Exposure.

    abstract：:The impact of low-dose ionizing radiation (IR) on the human brain has recently attracted attention due to the increased use of IR for diagnostic purposes. The aim of this study was to investigate low-dose radiation response in the hippocampus. Female B6C3F1 mice were exposed to total body irradiation with 0 (control),...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/acs.jproteome.9b00552

    authors： Hladik D,Dalke C,von Toerne C,Hauck SM,Azimzadeh O,Philipp J,Ung MC,Schlattl H,Rößler U,Graw J,Atkinson MJ,Tapio S

    更新日期：2020-01-03 00:00:00

  • Protein biomarkers for in vitro testing of embryotoxicity.

    abstract：:There are new challenges for hazard and risk assessment in the chemical industry with regard to REACH legislation in Europe and related activities in the U.S. and Japan, which require the development of novel in vitro models for the molecular characterization of drug- or chemical-related effects replacing conventional...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr100514e

    authors： Groebe K,Hayess K,Klemm-Manns M,Schwall G,Wozny W,Steemans M,Peters AK,Sastri C,Jaeckel P,Stegmann W,Zengerling H,Schopf R,Poznanovic S,Stummann TC,Seiler A,Spielmann H,Schrattenholz A

    更新日期：2010-11-05 00:00:00

  • Effects of Dietary Different Doses of Copper and High Fructose Feeding on Rat Fecal Metabolome.

    abstract：:The gut microbiota plays a critical role in the pathogenesis of nonalcoholic fatty liver disease (NAFLD). Increased fructose consumption and inadequate copper intake are two critical risk factors in the development of NAFLD. To gain insight into the role of gut microbiota, fecal metabolites, obtained from rats exposed...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/acs.jproteome.5b00596

    authors： Wei X,Song M,Yin X,Schuschke DA,Koo I,McClain CJ,Zhang X

    更新日期：2015-09-04 00:00:00

  • Evaluation of NCI-7 Cell Line Panel as a Reference Material for Clinical Proteomics.

    abstract：:Reference materials are vital to benchmarking the reproducibility of clinical tests and essential for monitoring laboratory performance for clinical proteomics. The reference material utilized for mass spectrometric analysis of the human proteome would ideally contain enough proteins to be suitably representative of t...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/acs.jproteome.8b00165

    authors： Clark DJ,Hu Y,Bocik W,Chen L,Schnaubelt M,Roberts R,Shah P,Whiteley G,Zhang H

    更新日期：2018-06-01 00:00:00

  • High-Resolution Mass Spectrometry Based Proteomic Analysis of the Response to Vancomycin-Induced Cell Wall Stress in Streptomyces coelicolor A3(2).

    abstract：:Understanding how bacteria survive periods of cell wall stress is of fundamental interest and can help generate ideas for improved antibacterial treatments. In this study we use tandem mass tagging to characterize the proteomic response of vancomycin resistant Streptomyces coelicolor to the exposure to sublethal level...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/acs.jproteome.5b00242

    authors： Hesketh A,Deery MJ,Hong HJ

    更新日期：2015-07-02 00:00:00

  • Phosphoproteomic analysis of human mesenchymal stromal cells during osteogenic differentiation.

    abstract：:Human mesenchymal stromal cells (hMSCs) are promising candidates for cell therapy and tissue regeneration. Knowledge of the molecular mechanisms governing hMSC commitment into osteoblasts is critical to the development of therapeutic applications for human bone diseases. Because protein phosphorylation plays a critica...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr200868p

    authors： Lo T,Tsai CF,Shih YR,Wang YT,Lu SC,Sung TY,Hsu WL,Chen YJ,Lee OK

    更新日期：2012-02-03 00:00:00

  • Urinary metabolite profiling combined with computational analysis predicts interstitial cystitis-associated candidate biomarkers.

    abstract：:Interstitial cystitis/painful bladder syndrome (IC) is a chronic syndrome of unknown etiology that presents with bladder pain, urinary frequency, and urgency. The lack of specific biomarkers and a poor understanding of underlying molecular mechanisms present challenges for disease diagnosis and therapy. The goals of t...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr5007729

    authors： Wen H,Lee T,You S,Park SH,Song H,Eilber KS,Anger JT,Freeman MR,Park S,Kim J

    更新日期：2015-01-02 00:00:00

  • B. subtilis ribosomal proteins: structural homology and post-translational modifications.

    abstract：:Ribosomal proteins of the model gram-positive bacterium B. subtilis 168 were extensively characterized in a proteomic study. Mass spectra of the 52 proteins expected to be constitutive components of the 70S ribosome were recorded. Peptide MS/MS analysis with an average sequence coverage of 85% supported the identifica...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr801114k

    authors： Lauber MA,Running WE,Reilly JP

    更新日期：2009-09-01 00:00:00

  • iTRAQ-based quantitative proteomics of developing and ripening muscadine grape berry.

    abstract：:Grapes are among the widely cultivated fruit crops in the world. Grape berries like other nonclimacteric fruits undergo a complex set of dynamic, physical, physiological, and biochemical changes during ripening. Muscadine grapes are widely cultivated in the southern United States for fresh fruit and wine. To date, cha...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr400731p

    authors： Kambiranda D,Katam R,Basha SM,Siebert S

    更新日期：2014-02-07 00:00:00

  • Investigating the impact of elevated levels of ozone on tropical wheat using integrated phenotypical, physiological, biochemical, and proteomics approaches.

    abstract：:Ozone (O3), a potent air pollutant and a significant greenhouse gas, has been recognized as a major component of global climate change. However, current increasing trends in its background level are projecting a more severe threat to natural and cultivated plants in the near future. The present study has been designed...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr1002824

    authors： Sarkar A,Rakwal R,Bhushan Agrawal S,Shibato J,Ogawa Y,Yoshida Y,Kumar Agrawal G,Agrawal M

    更新日期：2010-09-03 00:00:00

  • Characterization of Monophasic Solvent-Based Tissue Extractions for the Detection of Polar Metabolites and Lipids Applying Ultrahigh-Performance Liquid Chromatography-Mass Spectrometry Clinical Metabolic Phenotyping Assays.

    abstract：:Metabolic phenotyping of tissues uses metabolomics and lipidomics to measure the relative polar and nonpolar (lipid) metabolite levels in biological samples. This approach aims to understand disease biochemistry and identify biochemical markers of disease. Sample preparation methods must be reproducible, sensitive (hi...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/acs.jproteome.0c00660

    authors： Southam AD,Pursell H,Frigerio G,Jankevics A,Weber RJM,Dunn WB

    更新日期：2021-01-01 00:00:00

  • Minimizing Laboratory-Induced Decay in Bone Proteomics.

    abstract：:Proteomics methods are being increasingly used to study archaeological and palaeontological bone, assisting in species identification and phylogenetic studies as well as improving our understanding of bone diagenesis. More recently, there are developing interests in the study of post-translational modifications, some ...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/acs.jproteome.6b00564

    authors： Procopio N,Buckley M

    更新日期：2017-02-03 00:00:00

  • Proteomic identification of unique photoreceptor disc components reveals the presence of PRCD, a protein linked to retinal degeneration.

    abstract：:Visual signal transduction takes place on the surface of flat membrane vesicles called photoreceptor discs, which reside inside the light-sensitive outer segment organelle of vertebrate photoreceptor cells. Although biochemical studies have indicated that discs are built with a handful of highly specialized proteins, ...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr4003678

    authors： Skiba NP,Spencer WJ,Salinas RY,Lieu EC,Thompson JW,Arshavsky VY

    更新日期：2013-06-07 00:00:00

  • 'Signature sets', minimal fragment sets for identifying protein disulfide structures with cyanylation-based mass mapping methodology.

    abstract：:Our cyanylation (CN)-based methodology for determining disulfide structure of cystinyl proteins overcomes the limitations of conventional proteolytic methods. However, the CN-based method has the potential drawback that occasionally some CN-induced cleavage fragments may not be detected. We show that CN-based methods ...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr049961t

    authors： Wu W,Huang W,Qi J,Chou YT,Torng E,Watson JT

    更新日期：2004-07-01 00:00:00

  • Protein profilings in mouse liver regeneration after partial hepatectomy using iTRAQ technology.

    abstract：:Liver is unique in its capability to regenerate after an injury. Liver regeneration after a 2/3 partial hepatectomy served as a classical model and is adopted frequently to study the mechanism of liver regeneration. In the present study, semiquantitative analysis of protein expression in mouse liver regeneration follo...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr800696m

    authors： Hsieh HC,Chen YT,Li JM,Chou TY,Chang MF,Huang SC,Tseng TL,Liu CC,Chen SF

    更新日期：2009-02-01 00:00:00

  • Proteome mapping of human skim milk proteins in term and preterm milk.

    abstract：:The abundant proteins in human milk have been well characterized and are known to provide nutritional, protective, and developmental advantages to both term and preterm infants. However, relatively little is known about the expression of the low abundance proteins that are present in human milk because of the technica...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr2008797

    authors： Molinari CE,Casadio YS,Hartmann BT,Livk A,Bringans S,Arthur PG,Hartmann PE

    更新日期：2012-03-02 00:00:00

  • On-line concentration of microheterogeneous proteins by capillary electrophoresis using SDS and PEO as additives.

    abstract：:In this paper, we describe a method for analyzing large-volume protein samples using capillary electrophoresis in conjunction with laser-induced fluorescence detection (CE-LIF). To improve the stacking and separation efficiencies of proteins, we added either 0.01% sodium dodecyl sulfate (SDS) or 0.01% poly(ethylene ox...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr050298y

    authors： Huang YF,Hsieh MM,Tseng WL,Chang HT

    更新日期：2006-02-01 00:00:00

  • Proteomic comparison of historic and recently emerged hypervirulent Clostridium difficile strains.

    abstract：:Clostridium difficile in recent years has undergone rapid evolution and has emerged as a serious human pathogen. Proteomic approaches can improve the understanding of the diversity of this important pathogen, especially in comparing the adaptive ability of different C. difficile strains. In this study, TMT labeling an...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr3007528

    authors： Chen JW,Scaria J,Mao C,Sobral B,Zhang S,Lawley T,Chang YF

    更新日期：2013-03-01 00:00:00

  • Self-assembling protein arrays using electronic semiconductor microchips and in vitro translation.

    abstract：:Protein arrays will greatly accelerate research and development in medical and biological sciences. We have used cell-free protein biosynthesis and a parallel immobilization strategy for producing protein biochips. We demonstrate a model two-protein microarray using luciferase and green fluorescent protein, both expre...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr0300011

    authors： Oleinikov AV,Gray MD,Zhao J,Montgomery DD,Ghindilis AL,Dill K

    更新日期：2003-05-01 00:00:00

  • Evaluation of protein depletion methods for the analysis of total-, phospho- and glycoproteins in lumbar cerebrospinal fluid.

    abstract：:A proper sample preparation, in particular, abundant protein removal is crucial in the characterization of low-abundance proteins including those harboring post-translational modifications. In human cerebrospinal fluid (CSF), approximately 80% of proteins originate from serum, and removal of major proteins is necessar...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr049750o

    authors： Ogata Y,Charlesworth MC,Muddiman DC

    更新日期：2005-05-01 00:00:00

  • Proteomic analysis reveals a synergistic mechanism of fluconazole and berberine against fluconazole-resistant Candida albicans: endogenous ROS augmentation.

    abstract：:Our previous study showed that concomitant use of berberine (BBR) and fluconazole (FLC) provided a synergistic action against FLC-resistant Candida albicans (C. albicans) clinical strains in vitro. To clarify the mechanism underlying this action, we performed a comparative proteomic study in untreated control cells an...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr9005074

    authors： Xu Y,Wang Y,Yan L,Liang RM,Dai BD,Tang RJ,Gao PH,Jiang YY

    更新日期：2009-11-01 00:00:00

  • Serum Antibody Profile during Colonization of the Mouse Gut by Candida albicans: Relevance for Protection during Systemic Infection.

    abstract：:Candida albicans is a commensal microorganism in the oral cavity and gastrointestinal and urogenital tracts of most individuals that acts as an opportunistic pathogen when the host immune response is reduced. Here, we established different immunocompetent murine models to analyze the antibody responses to the C. albic...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/acs.jproteome.6b00383

    authors： Huertas B,Prieto D,Pitarch A,Gil C,Pla J,Díez-Orejas R

    更新日期：2017-01-06 00:00:00

  • In-gel derivatization of proteins for cysteine-specific cleavages and their analysis by mass spectrometry.

    abstract：:As a potential tool for proteomics and protein characterization, in-gel cysteine- and arginine-specific cleavage is demonstrated by means of trypsin or endoproteinase Lys-C for six model proteins (lysozyme, alpha-lactalbumin, beta-lactoglobulin, ribonuclease A, albumin, and transferrin), ranging in size from 14 kDa to...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr025568g

    authors： Thevis M,Ogorzalek Loo RR,Loo JA

    更新日期：2003-03-01 00:00:00

  • Proteomics of neuroendocrine secretory vesicles reveal distinct functional systems for biosynthesis and exocytosis of peptide hormones and neurotransmitters.

    abstract：:Regulated secretory vesicles produce, store, and secrete active peptide hormones and neurotransmitters that function in cell-cell communication. To gain knowledge of the protein systems involved in such secretory vesicle functions, we analyzed proteins in the soluble and membrane fractions of dense core secretory vesi...

    journal_title：Journal of proteome research

    pub_type： 杂志文章

    doi：10.1021/pr060503p

    authors： Wegrzyn J,Lee J,Neveu JM,Lane WS,Hook V

    更新日期：2007-05-01 00:00:00