Abstract：
:The impact of low-dose ionizing radiation (IR) on the human brain has recently attracted attention due to the increased use of IR for diagnostic purposes. The aim of this study was to investigate low-dose radiation response in the hippocampus. Female B6C3F1 mice were exposed to total body irradiation with 0 (control), 0.063, 0.125, or 0.5 Gy. Quantitative label-free proteomic analysis of the hippocampus was performed after 24 months. CREB signaling and CREB-associated pathways were affected at all doses. The lower doses (0.063 and 0.125 Gy) induced the CREB pathway, whereas the exposure to 0.5 Gy deactivated CREB. Similarly, the lowest dose (0.063 Gy) was anti-inflammatory, reducing the number of activated microglia. In contrast, induction of activated microglia and reactive astroglia was found at 0.5 Gy, suggesting increased inflammation and astrogliosis, respectively. The apoptotic markers BAX and cleaved CASP-3 and oxidative stress markers were increased only at the highest dose. Since the activated CREB pathway plays a central role in learning and memory, these data suggest neuroprotection at the lowest dose (0.063 Gy) but neurodegeneration at 0.5 Gy. The response to 0.5 Gy resembles alterations found in healthy aging and thus may represent radiation-induced accelerated aging of the brain.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Hladik D,Dalke C,von Toerne C,Hauck SM,Azimzadeh O,Philipp J,Ung MC,Schlattl H,Rößler U,Graw J,Atkinson MJ,Tapio Sdoi
10.1021/acs.jproteome.9b00552subject
Has Abstractpub_date
2020-01-03 00:00:00pages
337-345issue
1eissn
1535-3893issn
1535-3907journal_volume
19pub_type
杂志文章abstract：:A rate-limiting and costly step in many proteomics analyses is the cloning of all of the ORFs for an organism into technique-specific vectors. Here, we describe the generation of a Campylobacter jejuni expression clone set using a high-throughput cloning approach based on recombination in E. coli. The approach uses na...
journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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更新日期：2008-03-01 00:00:00
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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doi：10.1021/acs.jproteome.5b00443
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journal_title：Journal of proteome research
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更新日期：2017-08-04 00:00:00
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journal_title：Journal of proteome research
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更新日期：2014-12-05 00:00:00
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journal_title：Journal of proteome research
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更新日期：2010-07-02 00:00:00
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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更新日期：2007-11-01 00:00:00
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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更新日期：2011-02-04 00:00:00
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journal_title：Journal of proteome research
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journal_title：Journal of proteome research
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