CREB Signaling Mediates Dose-Dependent Radiation Response in the Murine Hippocampus Two Years after Total Body Exposure.


:The impact of low-dose ionizing radiation (IR) on the human brain has recently attracted attention due to the increased use of IR for diagnostic purposes. The aim of this study was to investigate low-dose radiation response in the hippocampus. Female B6C3F1 mice were exposed to total body irradiation with 0 (control), 0.063, 0.125, or 0.5 Gy. Quantitative label-free proteomic analysis of the hippocampus was performed after 24 months. CREB signaling and CREB-associated pathways were affected at all doses. The lower doses (0.063 and 0.125 Gy) induced the CREB pathway, whereas the exposure to 0.5 Gy deactivated CREB. Similarly, the lowest dose (0.063 Gy) was anti-inflammatory, reducing the number of activated microglia. In contrast, induction of activated microglia and reactive astroglia was found at 0.5 Gy, suggesting increased inflammation and astrogliosis, respectively. The apoptotic markers BAX and cleaved CASP-3 and oxidative stress markers were increased only at the highest dose. Since the activated CREB pathway plays a central role in learning and memory, these data suggest neuroprotection at the lowest dose (0.063 Gy) but neurodegeneration at 0.5 Gy. The response to 0.5 Gy resembles alterations found in healthy aging and thus may represent radiation-induced accelerated aging of the brain.


J Proteome Res


Hladik D,Dalke C,von Toerne C,Hauck SM,Azimzadeh O,Philipp J,Ung MC,Schlattl H,Rößler U,Graw J,Atkinson MJ,Tapio S




Has Abstract


2020-01-03 00:00:00












  • Insight into the salivary transcriptome and proteome of Dipetalogaster maxima.

    abstract::Dipetalogaster maxima is a blood-sucking Hemiptera that inhabits sylvatic areas in Mexico. It usually takes its blood meal from lizards, but following human population growth, it invaded suburban areas, feeding also on humans and domestic animals. Hematophagous insect salivary glands produce potent pharmacologic compo...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Assumpção TC,Charneau S,Santiago PB,Francischetti IM,Meng Z,Araújo CN,Pham VM,Queiroz RM,de Castro CN,Ricart CA,Santana JM,Ribeiro JM

    更新日期:2011-02-04 00:00:00

  • Gain-Scanning for Protein Microarray Assays.

    abstract::Protein microarrays consist of known proteins spotted onto solid substrates and are used to perform highly multivariate assessments of protein-binding interactions. Human protein arrays are routinely applied to pathogen detection, immune response biomarker profiling, and antibody specificity profiling. Here, we descri...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Feng F,Ataca ST,Ran M,Wang Y,Breen M,Kepler TB

    更新日期:2020-07-02 00:00:00

  • Radioactive Chernobyl environment has produced high-oil flax seeds that show proteome alterations related to carbon metabolism during seed development.

    abstract::Starting in 2007, we have grown soybean (Glycine max [L.] Merr. variety Soniachna) and flax (Linum usitatissimum, L. variety Kyivskyi) in the radio-contaminated Chernobyl area and analyzed the seed proteomes. In the second-generation flax seeds, we detected a 12% increase in oil content. To characterize the bases for ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Klubicová K,Danchenko M,Skultety L,Berezhna VV,Rashydov NM,Hajduch M

    更新日期:2013-11-01 00:00:00

  • A new workflow for proteomic analysis of urinary exosomes and assessment in cystinuria patients.

    abstract::Cystinuria is a purely renal, rare genetic disease caused by mutations in cystine transporter genes and characterized by defective cystine reabsorption leading to kidney stones. In 14% of cases, patients undergo nephrectomy, but given the difficulty to predict the evolution of the disease, the identification of marker...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Bourderioux M,Nguyen-Khoa T,Chhuon C,Jeanson L,Tondelier D,Walczak M,Ollero M,Bekri S,Knebelmann B,Escudier E,Escudier B,Edelman A,Guerrera IC

    更新日期:2015-01-02 00:00:00

  • Quantitative Proteomics Reveals Potential Crosstalk between a Small RNA CoaR and a Two-Component Regulator Slr1037 in Synechocystis sp. PCC6803.

    abstract::Bacterial small RNAs (sRNAs) and two-component systems (TCSs) were two vital regulatory mechanisms employed by microorganisms to respond to environmental changes and stresses. As a promising "autotrophic cell factory", photosynthetic cyanobacteria have attracted a lot of attention these years. Although most studies fo...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Sun T,Chen L,Zhang W

    更新日期:2017-08-04 00:00:00

  • Identification of novel protein biomarkers of preterm birth in human cervical-vaginal fluid.

    abstract::Spontaneous preterm birth (SPTB) is a major contributor to perinatal morbidity and mortality. However, the diagnosis of preterm labor (PTL) that leads to preterm birth is difficult, and there is a pressing need for improved diagnosis. We utilized multidimensional liquid chromatography-tandem mass spectrometry (LC/LC-M...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Pereira L,Reddy AP,Jacob T,Thomas A,Schneider KA,Dasari S,Lapidus JA,Lu X,Rodland M,Roberts CT Jr,Gravett MG,Nagalla SR

    更新日期:2007-04-01 00:00:00

  • Automated N-glycopeptide identification using a combination of single- and tandem-MS.

    abstract::We describe Peptoonist, a program that can automatically identify the glycans (sugars) present at each N-glycosylation site of a protein. The input to Peptoonist is a series of mass spectra, both MS and MS/MS, obtained from a liquid chromatography (LC) run of proteolytically digested purified glycoproteins. The progra...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Goldberg D,Bern M,Parry S,Sutton-Smith M,Panico M,Morris HR,Dell A

    更新日期:2007-10-01 00:00:00

  • Proteomic evidence for roles for nucleolin and poly[ADP-ribosyl] transferase in drug resistance.

    abstract::One-hundred twenty-four proteins have been identified in the soluble nuclear protein mixture from MCF-7 human breast cancer cells, of which more than 90% are classically categorized as nuclear proteins. Proteins were also studied from three drug resistant MDF-7 lines, selected previously from the same parent line by e...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Fu Z,Fenselau C

    更新日期:2005-09-01 00:00:00

  • Parallel tandem: a program for parallel processing of tandem mass spectra using PVM or MPI and X!Tandem.

    abstract::A method for the rapid correlation of tandem mass spectra to a list of protein sequences in a database has been developed. The combination of the fast and accurate computational search algorithm, X!Tandem, and a Linux cluster parallel computing environment with PVM or MPI, significantly reduces the time required to pe...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Duncan DT,Craig R,Link AJ

    更新日期:2005-09-01 00:00:00

  • Feasibility of detecting prostate cancer by ultraperformance liquid chromatography-mass spectrometry serum metabolomics.

    abstract::Prostate cancer (PCa) is the second leading cause of cancer-related mortality in men. The prevalent diagnosis method is based on the serum prostate-specific antigen (PSA) screening test, which suffers from low specificity, overdiagnosis, and overtreatment. In this work, untargeted metabolomic profiling of age-matched ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Zang X,Jones CM,Long TQ,Monge ME,Zhou M,Walker LD,Mezencev R,Gray A,McDonald JF,Fernández FM

    更新日期:2014-07-03 00:00:00

  • Advances in Higher Order Multiplexing Techniques in Proteomics.

    abstract::Proteomics by mass spectrometry (MS) allows the large-scale identification and quantitation of the cellular proteins in a given biological context. Systems biology studies from proteomics data are largely limited by the accuracy and coverage of quantitative proteomics along with missing values. Toward this end, statis...

    journal_title:Journal of proteome research

    pub_type: 杂志文章,评审


    authors: Aggarwal S,Talukdar NC,Yadav AK

    更新日期:2019-06-07 00:00:00

  • PhosphoScore: an open-source phosphorylation site assignment tool for MSn data.

    abstract::Correct phosphorylation site assignment is a critical aspect of phosphoproteomic analysis. Large-scale phosphopeptide data sets that are generated through liquid chromatography-coupled tandem mass spectrometry (LC-MS/MS) analysis often contain hundreds or thousands of phosphorylation sites that require validation. To ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Ruttenberg BE,Pisitkun T,Knepper MA,Hoffert JD

    更新日期:2008-07-01 00:00:00

  • Snake venomics of Bothriechis nigroviridis reveals extreme variability among palm pitviper venoms: different evolutionary solutions for the same trophic purpose.

    abstract::We report the proteomic characterization and biological activities of the venom of the black-speckled palm pitviper, Bothriechis nigroviridis, a neotropical arboreal pitviper from Costa Rica. In marked contrast to other Bothriechis species investigated, the venom of B. nigroviridis does not possess detectable Zn(2+)-d...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Fernández J,Lomonte B,Sanz L,Angulo Y,Gutiérrez JM,Calvete JJ

    更新日期:2010-08-06 00:00:00

  • Proteins associated with immunopurified granules from a model pancreatic islet beta-cell system: proteomic snapshot of an endocrine secretory granule.

    abstract::beta-Cell granules contain proteins involved in fuel regulation, which when altered, contribute to metabolic disorders including diabetes mellitus. We analyzed proteins present in purified granules from the INS-1E beta-cell model. Fifty-one component proteins were identified by LC-MS/MS including hormones, granins, pr...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Hickey AJ,Bradley JW,Skea GL,Middleditch MJ,Buchanan CM,Phillips AR,Cooper GJ

    更新日期:2009-01-01 00:00:00

  • Membrane proteomic analysis of Arabidopsis thaliana using alternative solubilization techniques.

    abstract::This study presents a comparative proteomic analysis of the membrane subproteome of whole Arabidopsis seedlings using 2% Brij-58 or 60% methanol to enrich and solubilize membrane proteins for strong cation exchange fractionation and reversed-phase liquid chromatography-tandem mass spectrometry (LC-MS/MS). A total of 4...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Mitra SK,Gantt JA,Ruby JF,Clouse SD,Goshe MB

    更新日期:2007-05-01 00:00:00

  • Workflow for Integrated Processing of Multicohort Untargeted 1H NMR Metabolomics Data in Large-Scale Metabolic Epidemiology.

    abstract::Large-scale metabolomics studies involving thousands of samples present multiple challenges in data analysis, particularly when an untargeted platform is used. Studies with multiple cohorts and analysis platforms exacerbate existing problems such as peak alignment and normalization. Therefore, there is a need for robu...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Karaman I,Ferreira DL,Boulangé CL,Kaluarachchi MR,Herrington D,Dona AC,Castagné R,Moayyeri A,Lehne B,Loh M,de Vries PS,Dehghan A,Franco OH,Hofman A,Evangelou E,Tzoulaki I,Elliott P,Lindon JC,Ebbels TM

    更新日期:2016-12-02 00:00:00

  • Nontargeted urinary metabolite profiling of a mouse model of Crohn's disease.

    abstract::Crohn's disease is an inflammatory disorder of the bowel, believed to arise from the dysregulation of intestinal mucosal immunity. The interleukin-10-deficient (IL10-/-) mouse, which develops intestinal inflammation in the presence of gut microflora, serves as a mouse model of Crohn's disease. Nontargeted urinary meta...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Lin HM,Edmunds SI,Helsby NA,Ferguson LR,Rowan DD

    更新日期:2009-04-01 00:00:00

  • Plasma protein N-glycan profiles are associated with calendar age, familial longevity and health.

    abstract::The development of medical interventions for the preservation of disease-free longevity would be facilitated by markers that predict healthy aging. Altered protein N-glycosylation patterns have been found with increasing age and several disease states. Here we investigate whether glycans derived from the total glycopr...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Ruhaak LR,Uh HW,Beekman M,Hokke CH,Westendorp RG,Houwing-Duistermaat J,Wuhrer M,Deelder AM,Slagboom PE

    更新日期:2011-04-01 00:00:00

  • Computational and Mass-Spectrometry-Based Workflow for the Discovery and Validation of Missing Human Proteins: Application to Chromosomes 2 and 14.

    abstract::In the framework of the C-HPP, our Franco-Swiss consortium has adopted chromosomes 2 and 14, coding for a total of 382 missing proteins (proteins for which evidence is lacking at protein level). Over the last 4 years, the French proteomics infrastructure has collected high-quality data sets from 40 human samples, incl...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Carapito C,Lane L,Benama M,Opsomer A,Mouton-Barbosa E,Garrigues L,Gonzalez de Peredo A,Burel A,Bruley C,Gateau A,Bouyssié D,Jaquinod M,Cianferani S,Burlet-Schiltz O,Van Dorsselaer A,Garin J,Vandenbrouck Y

    更新日期:2015-09-04 00:00:00

  • gFinder: A Web-Based Bioinformatics Tool for the Analysis of N-Glycopeptides.

    abstract::Glycoproteins influence numerous indispensable biological functions, and changes in protein glycosylation have been observed in various diseases. The identification and characterization of glycoprotein and glycosylation sites by mass spectrometry (MS) remain challenging tasks, and great efforts have been devoted to th...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Kim JW,Hwang H,Lim JS,Lee HJ,Jeong SK,Yoo JS,Paik YK

    更新日期:2016-11-04 00:00:00

  • Proteomic Analysis of Peripheral Blood Mononuclear Cells after a High-Fat, High-Carbohydrate Meal with Orange Juice.

    abstract::Oxidative stress and inflammation play a role in the physiopathology of insulin resistance, diabetes and cardiovascular disease. A single high-fat, high-carbohydrate (HFHC) meal induces an increase in inflammatory and oxidative stress markers in peripheral blood mononuclear cells (PBMC). Previous studies have shown th...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Chaves DFS,Carvalho PC,Brasili E,Rogero MM,Hassimotto NA,Diedrich JK,Moresco JJ,Yates JR 3rd,Lajolo FM

    更新日期:2017-11-03 00:00:00

  • Improved immobilized metal affinity chromatography for large-scale phosphoproteomics applications.

    abstract::Dysregulated protein phosphorylation is a primary culprit in multiple physiopathological states. Hence, although analysis of signaling cascades on a proteome-wide scale would provide significant insight into both normal and aberrant cellular function, such studies are simultaneously limited by sheer biological complex...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Ndassa YM,Orsi C,Marto JA,Chen S,Ross MM

    更新日期:2006-10-01 00:00:00

  • Altered chaperone and protein turnover regulators expression in cultured skin fibroblasts from type 1 diabetes mellitus with nephropathy.

    abstract::In type-1 diabetes mellitus (T1DM) with diabetic nephropathy (DN), accumulation of abnormal proteins in the kidney and other tissues may derive from constitutive alterations of intracellular protein recognition, assembly, and turnover. We characterized the proteins involved in these functions in cultured skin fibrobla...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Tessari P,Puricelli L,Iori E,Arrigoni G,Vedovato M,James P,Coracina A,Millioni R

    更新日期:2007-03-01 00:00:00

  • Detecting Modifications in Proteomics Experiments with Param-Medic.

    abstract::Searching tandem mass spectra against a peptide database requires accurate knowledge of various experimental parameters, including machine settings and details of the sample preparation protocol. In some cases, such as in reanalysis of public data sets, this experimental metadata may be missing or inaccurate. We descr...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: May DH,Tamura K,Noble WS

    更新日期:2019-04-05 00:00:00

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    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Emmott E,Wise H,Loucaides EM,Matthews DA,Digard P,Hiscox JA

    更新日期:2010-10-01 00:00:00

  • Comparison of three quantitative phosphoproteomic strategies to study receptor tyrosine kinase signaling.

    abstract::There are three quantitative phosphoproteomic strategies most commonly used to study receptor tyrosine kinase (RTK) signaling. These strategies quantify changes in: (1) all three forms of phosphosites (phosphoserine, phosphothreonine and phosphotyrosine) following enrichment of phosphopeptides by titanium dioxide or i...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Zhang G,Neubert TA

    更新日期:2011-12-02 00:00:00

  • Proteomic analysis of high-grade dysplastic cervical cells obtained from ThinPrep slides using laser capture microdissection and mass spectrometry.

    abstract::The purpose of this discovery phase study was to identify candidate protein biomarkers for high-grade dysplastic cervical cells using mass spectrometry. Laser Capture Microdissection (LCM) was utilized to isolate high-grade dysplastic and normal cells from ThinPrep slides prepared from cervical cytological specimens. ...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Gu Y,Wu SL,Meyer JL,Hancock WS,Burg LJ,Linder J,Hanlon DW,Karger BL

    更新日期:2007-11-01 00:00:00

  • Red Blood Cell Metabolic Responses to Torpor and Arousal in the Hibernator Arctic Ground Squirrel.

    abstract::Arctic ground squirrels provide a unique model to investigate metabolic responses to hibernation in mammals. During winter months these rodents are exposed to severe hypothermia, prolonged fasting, and hypoxemia. In the light of their role in oxygen transport/off-loading and owing to the absence of nuclei and organell...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Gehrke S,Rice S,Stefanoni D,Wilkerson RB,Nemkov T,Reisz JA,Hansen KC,Lucas A,Cabrales P,Drew K,D'Alessandro A

    更新日期:2019-04-05 00:00:00

  • Exoproteome Heterogeneity among Closely Related Staphylococcus aureus t437 Isolates and Possible Implications for Virulence.

    abstract::Staphylococcus aureus with spa-type t437 has been identified as a predominant community-associated methicillin-resistant S. aureus clone from Asia, which is also encountered in Europe. Molecular typing has previously shown that t437 isolates are highly similar regardless of geographical regions or host environments. T...

    journal_title:Journal of proteome research

    pub_type: 杂志文章


    authors: Zhao X,Palma Medina LM,Stobernack T,Glasner C,de Jong A,Utari P,Setroikromo R,Quax WJ,Otto A,Becher D,Buist G,van Dijl JM

    更新日期:2019-07-05 00:00:00

  • Mitochondrial Function, Metabolic Regulation, and Human Disease Viewed through the Prism of Sirtuin 4 (SIRT4) Functions.

    abstract::As cellular metabolic hubs, mitochondria are the main energy producers for the cell. These organelles host essential energy producing biochemical processes, including the TCA cycle, fatty acid oxidation, and oxidative phosphorylation. An accumulating body of literature has demonstrated that a majority of mitochondrial...

    journal_title:Journal of proteome research

    pub_type: 杂志文章,评审


    authors: Betsinger CN,Cristea IM

    更新日期:2019-05-03 00:00:00