Abstract:
:Porcine reproductive and respiratory syndrome virus (PRRSV) is a major threat to the swine industry worldwide and hence global food security, exacerbated by a newly emerged highly pathogenic (HP-PRRSV) strain from China. PRRSV nonstructural protein 2 (nsp2) is a multifunctional polypeptide with strain-dependent influences on pathogenicity. A number of discrete functional regions have been identified on the protein. Quantitative label free proteomics was used to identify cellular binding partners of nsp2 expressed by HP-PRRSV. This allowed the identification of potential cellular interacting partners and the discrimination of nonspecific interactions. The interactome data were further investigated and validated using biological replicates and also compared with nsp2 from a low pathogenic (LP) strain of PRRSV. Validation included both forward and reverse pulldowns and confocal microscopy. The data indicated that nsp2 interacted with a number of cellular proteins including 14-3-3, CD2AP, and other components of cellular aggresomes. The hyper-variable region of nsp2 protein was identified as a binding platform for association with 14-3-3 proteins.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Xiao Y,Wu W,Gao J,Smith N,Burkard C,Xia D,Zhang M,Wang C,Archibald A,Digard P,Zhou EM,Hiscox JAdoi
10.1021/acs.jproteome.5b00396subject
Has Abstractpub_date
2016-05-06 00:00:00pages
1388-401issue
5eissn
1535-3893issn
1535-3907journal_volume
15pub_type
杂志文章abstract::A major source of intestinal metabolites results from both host and microbial processing of dietary nutrients. (1)H NMR-based metabolic profiling of mouse feces was carried out over time in different microbiome mouse models, including conventional (n = 9), conventionalized (n = 10), and "humanized" gnotobiotic mice in...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr100554m
更新日期:2010-10-01 00:00:00
abstract::The use of matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) to acquire spectral profiles has become a common approach to detect proteomic biomarkers of disease. MALDI-MS signals may represent both intact proteins as well as proteolysis products. Liquid chromatography-tandem mass spectrometry (L...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr050138m
更新日期:2005-09-01 00:00:00
abstract::Proteomics has been applied to study intracellular bacteria and phagocytic vacuoles in different host cell lines, especially macrophages (Mφs). For mycobacterial phagosomes, few studies have identified over several hundred proteins for systems assessment of the phagosome maturation and antigen presentation pathways. M...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr101245u
更新日期:2011-05-06 00:00:00
abstract::Propranolol is a nonselective beta-blocker of the beta-adrenergic receptors, and the S-enantiomer is more active compared with the R-enantiomer. Clinically, it has been shown to be effective in hypermetabolic burn patients by decreasing cardiac work, protein catabolism, and lipolysis. While gene expression profiles ha...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr0605926
更新日期:2007-05-01 00:00:00
abstract::Target-decoy approach (TDA) is the dominant strategy for false discovery rate (FDR) estimation in mass-spectrometry-based proteomics. One of its main applications is direct FDR estimation based on counting of decoy matches above a certain score threshold. The corresponding equations are widely employed for filtering o...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.6b00144
更新日期:2017-02-03 00:00:00
abstract::Triply and doubly charged iTRAQ ( isobaric tagging for relative and absolute quantitation) labeled peptide cations from a tryptic peptide mixture of bovine carbonic anhydrase II were subjected to electron transfer ion/ion reactions to investigate the effect of charge bearing modifications associated with iTRAQ on the ...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr8001113
更新日期:2008-09-01 00:00:00
abstract::The low selectivity of Kv1 peptide inhibitors for specific isoforms makes them poor candidates for the development of theraputics. Using combined approaches, we showed that the Kv1 turret is the critical determinant for ADWX-1 peptide inhibitor selectivity of Kv1.3 over Kv1.1. Mutation of Kv1.1 turret residues to matc...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr800494a
更新日期:2008-11-01 00:00:00
abstract::Lactic acid bacteria are broadly employed as starter cultures in the manufacture of foods. Upon technological preparation, they are confronted with drying stress that amalgamates numerous stress conditions resulting in losses of fitness and survival. To better understand and differentiate physiological stress response...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.7b00474
更新日期:2017-10-06 00:00:00
abstract::Hepatocellular carcinoma (HCC) is a major liver malignancy possessing a high mortality rate and is particularly prevalent in China and Asia. While surgery is the most effective treatment for liver tumor, about 80% of HCC patients are inoperable at presentation and die early due to late diagnosis. For early cancer dete...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr9002118
更新日期:2010-01-01 00:00:00
abstract::Preeclampsia is a pregnancy-specific hypertensive syndrome and a major cause of maternal and fetal morbidity and mortality. At the present time, no reliable screening tests to identify women at risk are available. We have compared the amniotic fluids (AF) proteomic maps of five preeclamptic patients with those of five...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr060315z
更新日期:2007-01-01 00:00:00
abstract::Biomarkers for colorectal cancer (CRC) early diagnosis are currently lacking. The purpose of this study was to interpret molecular events in the early stage of CRC that may bring about new biomarkers for early diagnosis. Methylation isotope labeling assistant gel-enhanced liquid chromatography-mass spectrometry (GeLC-...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr100406z
更新日期:2010-09-03 00:00:00
abstract::The mechanisms whereby Mycobacterium tuberculosis (Mtb) rewires the host metabolism in vivo are surprisingly unexplored. Here, we used three high-resolution mass spectrometry platforms to track altered lung metabolic changes associated with Mtb infection of mice. The multiplatform data sets were merged using consensus...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.9b00868
更新日期:2020-05-01 00:00:00
abstract::As a potential tool for proteomics and protein characterization, in-gel cysteine- and arginine-specific cleavage is demonstrated by means of trypsin or endoproteinase Lys-C for six model proteins (lysozyme, alpha-lactalbumin, beta-lactoglobulin, ribonuclease A, albumin, and transferrin), ranging in size from 14 kDa to...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr025568g
更新日期:2003-03-01 00:00:00
abstract::Bacillus Amyloliquefaciens SQR9 is a well-investigated plant growth-promoting rhizobacteria with strong root colonization capability. To identify the key proteins involved in in situ root colonization and biofilm formation, the proteomic profiles of planktonic and root colonized SQR9 cells were compared. A total of 75...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr500565m
更新日期:2014-12-05 00:00:00
abstract::Amyotrophic lateral sclerosis (ALS) is characterized by the absence of reliable diagnostic biomarkers. The aim of the study was to (i) devise an untargeted metabolomics methodology that reliably compares cerebrospinal fluid (CSF) from ALS patients and controls by liquid chromatography coupled to high-resolution mass s...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr400376e
更新日期:2013-08-02 00:00:00
abstract::Histones are well-conserved proteins that form the basic structure of chromatin in eukaryotes and undergo several post-translational modifications, which are important for the control of transcription, replication, DNA damage repair, and chromosome condensation. In early branched organisms, histones are less conserved...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.6b00208
更新日期:2016-06-03 00:00:00
abstract::One of the most thoroughly studied insect species, with respect to locomotion behavior, is the stick insect Carausius morosus. Although detailed information exists on premotor networks controlling walking, surprisingly little is known about neuropeptides, which are certainly involved in motor activity generation and m...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.8b00155
更新日期:2018-06-01 00:00:00
abstract::Acinetobacter baumannii causes severe infections in compromised patients. We combined SDS-PAGE, two-dimensional gel electrophoresis and mass spectrometry (LC-MS/MS and MALDI-TOF) to separate and characterize the proteins of the cell envelope of this bacterium. In total, 135 proteins (inner and outer membrane proteins)...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr060372s
更新日期:2006-12-01 00:00:00
abstract::Fulminant type 1 diabetes (FT1DM) is a relatively new clinical entity featured by acute destruction of pancreatic beta cells. Clinical consequences of FT1DM could be fatal when timely medications are not provided, suggesting the particular importance of rapid and accurate diagnosis. Here we report a serum metabonomics...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr300523x
更新日期:2012-09-07 00:00:00
abstract::We assessed proteomic patterns in breast cancer using MALDI MS and laser capture microdissected cells. Protein and peptide expression in invasive mammary carcinoma versus normal mammary epithelium and estrogen-receptor positive versus estrogen-receptor negative tumors were compared. Biomarker candidates were identifie...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr7008109
更新日期:2008-04-01 00:00:00
abstract::High-affinity antibodies binding to linear peptides in solution are a prerequisite for performing immuno-MRM, an emerging technology for protein quantitation with high precision and specificity using peptide immunoaffinity enrichment coupled to stable isotope dilution and targeted mass spectrometry. Recombinant antibo...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr4009404
更新日期:2014-04-04 00:00:00
abstract::The ThinPrep cervical smear is widely used in clinical practice for the cytological and molecular screening against abnormal cells and Human Papillomavirus (HPV) infection. Current advancements made to LC-MS proteomics include the use of stable isotope labeling for the in-depth analysis of proteins in complex clinical...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr301067r
更新日期:2013-05-03 00:00:00
abstract::The development of plasma biomarkers has proven to be more challenging than initially anticipated. Many studies have reported lists of candidate proteins rather than validated candidate markers with an assigned performance to a specific clinical objective. Biomarker research necessitates a clear rational framework wit...
journal_title:Journal of proteome research
pub_type: 杂志文章,评审
doi:10.1021/pr1008515
更新日期:2011-01-07 00:00:00
abstract::Correct phosphorylation site assignment is a critical aspect of phosphoproteomic analysis. Large-scale phosphopeptide data sets that are generated through liquid chromatography-coupled tandem mass spectrometry (LC-MS/MS) analysis often contain hundreds or thousands of phosphorylation sites that require validation. To ...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr800169k
更新日期:2008-07-01 00:00:00
abstract::Defects in the gene encoding the persulfide dioxygenase ETHE1 are known to cause the severe inherited metabolic disorder ethylmalonic encephalopathy (EE). In spite of known clinical characteristics, the molecular mechanisms underlying the ETHE1 deficiency are still obscure. Herein, to further analyze the molecular phe...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.6b00100
更新日期:2016-05-06 00:00:00
abstract::Huntington's disease (HD) is an autosomal dominant neurodegenerative disorder caused by a mutation in the Huntingtin gene, where excessive (≥ 36) CAG repeats encode for glutamine expansion in the huntingtin protein. Research using mouse models and human pathological material has indicated dysfunctions in a myriad of s...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr500649m
更新日期:2014-12-05 00:00:00
abstract::Regulated secretory vesicles produce, store, and secrete active peptide hormones and neurotransmitters that function in cell-cell communication. To gain knowledge of the protein systems involved in such secretory vesicle functions, we analyzed proteins in the soluble and membrane fractions of dense core secretory vesi...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr060503p
更新日期:2007-05-01 00:00:00
abstract::Cancers are initiated and developed from a small population of stem-like cells termed cancer stem cells (CSCs). There is heterogeneity among this CSC population that leads to multiple subpopulations with their own distinct biological features and protein expression. The protein expression and function may be impacted ...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/acs.jproteome.6b00824
更新日期:2017-02-03 00:00:00
abstract::Increased serum haptoglobin concentration and changes in its glycosylation have been reported in certain cancer types. Information for hepatocellular carcinoma (HCC) has not yet been available. In this study, we aimed to carry out a systematic analysis of serum concentrations of haptoglobin (Hp) and its glycoforms in ...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr060109r
更新日期:2006-10-01 00:00:00
abstract::We report the proteomic characterization and biological activities of the venom of the black-speckled palm pitviper, Bothriechis nigroviridis, a neotropical arboreal pitviper from Costa Rica. In marked contrast to other Bothriechis species investigated, the venom of B. nigroviridis does not possess detectable Zn(2+)-d...
journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr100545d
更新日期:2010-08-06 00:00:00