Abstract:
:"Dry eye" is a multifactorial inflammatory disease affecting the ocular surface. Tear hyperosmolarity in dry eye contributes to inflammation and cell damage. Recent research efforts on dry eye have been directed toward biomarker discovery for diagnosis, response to treatment, and disease mechanisms. This study employed a spontaneously immortalized normal human conjunctival cell line, IOBA-NHC, as a model to investigate hyperosmotic stress-induced changes of metabolites and proteins. Global and targeted metabonomic analyses as well as proteomic analysis were performed on IOBA-NHC cells incubated in serum-free media at 280 (control), 380, and 480 mOsm for 24 h. Twenty-one metabolites and seventy-six iTRAQ-identified proteins showed significant changes under at least one hyperosmotic stress treatment as compared with controls. SWATH-based proteomic analysis further confirmed the involvement of inflammatory pathways such as prostaglandin 2 synthesis in IOBA-NHC cells under hyperosmotic stress. This study is the first to identify glycerophosphocholine synthesis and O-linked β-N-acetylglucosamine glycosylation as key activated pathways in ocular surface cells under hyperosmotic stress. These findings extend the current knowledge in metabolite markers of dry eye and provide potential therapeutic targets for its treatment.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Chen L,Li J,Guo T,Ghosh S,Koh SK,Tian D,Zhang L,Jia D,Beuerman RW,Aebersold R,Chan EC,Zhou Ldoi
10.1021/acs.jproteome.5b00443subject
Has Abstractpub_date
2015-09-04 00:00:00pages
3982-95issue
9eissn
1535-3893issn
1535-3907journal_volume
14pub_type
杂志文章abstract::A proper sample preparation, in particular, abundant protein removal is crucial in the characterization of low-abundance proteins including those harboring post-translational modifications. In human cerebrospinal fluid (CSF), approximately 80% of proteins originate from serum, and removal of major proteins is necessar...
journal_title:Journal of proteome research
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pub_type: 杂志文章
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journal_title:Journal of proteome research
pub_type: 杂志文章
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更新日期:2015-12-04 00:00:00
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journal_title:Journal of proteome research
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journal_title:Journal of proteome research
pub_type: 杂志文章
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更新日期:2018-07-06 00:00:00
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journal_title:Journal of proteome research
pub_type: 杂志文章
doi:10.1021/pr9005662
更新日期:2009-10-01 00:00:00
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journal_title:Journal of proteome research
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doi:10.1021/pr0499040
更新日期:2004-11-01 00:00:00
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journal_title:Journal of proteome research
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journal_title:Journal of proteome research
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更新日期:2012-11-02 00:00:00
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journal_title:Journal of proteome research
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journal_title:Journal of proteome research
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