Abstract：
:Tear proteome profiling may generate useful information for the understanding of the interaction between an eye and its contacting objects, such as a contact lens or a lens implant. This is important for designing improved eye-care devices and maintaining the health of an eye. Proteome profiles of tear fluids may also be used for disease diagnosis and prognosis. However, only a small volume of tear fluid (<5 microL) can be collected in a clinical laboratory under normal operational conditions, which makes proteome profiling a challenge. In this work we apply several proteomic analysis techniques, including gel-based and solution-based approaches with LC-ESI and LC-MALDI MS and MS/MS to gauge the relative merits of producing proteome profiles and to generate as broad a coverage of the tear proteome as possible from this small amount of sample. It is shown that a total of 54 proteins can be confidently identified using less than 5 microL of tear fluid. Of these, 44 proteins can be detected by LC-MALDI MS alone with a consumption of 2 microL of tear fluid. Furthermore, LC-MALDI can be used to determine post-translational modifications (PTMs), such as glycosylation and phosphorylation, without any sample enrichment or treatment. This work represents one of the most extensive proteome profiles (i.e., proteins identified and PTMs characterized) generated from tear fluids using clinically relevant amounts of sample.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Li N,Wang N,Zheng J,Liu XM,Lever OW,Erickson PM,Li Ldoi
10.1021/pr0501970keywords:
subject
Has Abstractpub_date
2005-11-01 00:00:00pages
2052-61issue
6eissn
1535-3893issn
1535-3907journal_volume
4pub_type
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更新日期：2007-04-01 00:00:00
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journal_title：Journal of proteome research
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更新日期：2004-05-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.5b01031
更新日期：2016-07-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/acs.jproteome.5b00219
更新日期：2015-07-02 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
doi：10.1021/pr800854t
更新日期：2009-05-01 00:00:00
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更新日期：2014-09-05 00:00:00
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更新日期：2007-03-01 00:00:00
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journal_title：Journal of proteome research
pub_type： 杂志文章
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更新日期：2014-03-07 00:00:00
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journal_title：Journal of proteome research
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doi：10.1021/acs.jproteome.6b00022
更新日期：2016-04-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2019-05-03 00:00:00
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更新日期：2008-03-01 00:00:00
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更新日期：2012-11-02 00:00:00
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更新日期：2007-05-01 00:00:00
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pub_type： 杂志文章
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更新日期：2017-09-01 00:00:00
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更新日期：2019-12-06 00:00:00
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更新日期：2010-04-05 00:00:00
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