Abstract:
:Antibody reagents have been remained as a standard approach to characterize blood group (BG) antigens in clinic. The specificity and cross-reactivity of these BG antibodies are routine detected using the gel microcolumn assay (GMA). However, the GMA is neither specific nor sensitive, thus increasing the risk of improperly matched RBC transfusions. In this work, we describe a bead-based RBC membrane antigen array to detect BG antibody-antigen binding with ∼700-fold higher sensitivity and dynamic range than the GMA. RBC membrane antigen arrays were fabricated using fragmented RBC membranes highly enriched in BG panel antigens. The arrays were then used to screen the interactions of 15 BG reagents to three antigen panels. The majority of the antibody reactions (i.e., 86.7%; 39/45) aligned with those obtained with the GMA. The six cross-reactive, nonspecific antibody reactions identified only by our arrays (i.e., 13.3%; 6/45) were confirmed by agglutination inhibition and genotyping assays. These results demonstrate that our RBC membrane antigen array has great potential in screening BG antibodies and improving the safety of RBC transfusions.
journal_name
J Proteome Resjournal_title
Journal of proteome researchauthors
Yang L,Yu Y,Ma C,Wang H,Dai J,Duan H,Fu Z,Wu P,Wang D,Yu Xdoi
10.1021/acs.jproteome.8b00370subject
Has Abstractpub_date
2018-09-07 00:00:00pages
3237-3245issue
9eissn
1535-3893issn
1535-3907journal_volume
17pub_type
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