Advantages of tandem LC-MS for the rapid assessment of tissue-specific metabolic complexity using a pentafluorophenylpropyl stationary phase.

abstract：:Linear, short-chain polyfluorinated and perfluorinated alkyl compounds, often referred to as PFCs, have been in worldwide use as surfactants and polymer precursors for decades, and environmental dispersal of these highly persistent compounds represents a public health threat. Whereas ubiquitous low-level exposure to t...

journal_title：Journal of proteome research

authors： Hansmeier N,Chao TC,Herbstman JB,Goldman LR,Witter FR,Halden RU

更新日期：2015-01-02 00:00:00

abstract：:In humans, the olfactory epithelium is located in two narrow passages, the olfactory clefts, at the upper part of the nasal cavities. The olfactory epithelium is covered by a mucus layer which is essential for the function of the olfactory neurons that are directly connected with the brain through the cribriform plate...

journal_title：Journal of proteome research

authors： Débat H,Eloit C,Blon F,Sarazin B,Henry C,Huet JC,Trotier D,Pernollet JC

更新日期：2007-05-01 00:00:00

abstract：:Human embryonic stem cells (hESCs) are promising in regenerative medicine (RM) due to their differentiation plasticity and proliferation potential. However, a major challenge in RM is the generation of a vascular system to support nutrient flow to newly synthesized tissues. Here we refined an existing method to genera...

journal_title：Journal of proteome research

authors： Tsolis KC,Bagli E,Kanaki K,Zografou S,Carpentier S,Bei ES,Christoforidis S,Zervakis M,Murphy C,Fotsis T,Economou A

更新日期：2016-06-03 00:00:00

abstract：:Tryptic digestion of biotinylated Lys-C peptides followed by affinity chromatography allows the selective isolation of lysine-free tryptic peptides delimited by arginine residues (RRnK peptides). In silico analysis revealed that RRnK peptides represent 87% of the whole proteomes and their specific isolation simplifies...

journal_title：Journal of proteome research

authors： Sánchez A,González LJ,Ramos Y,Betancourt L,Gil J,Besada V,Fernández-de-Cossio J,Alvarez F,Padrón G

更新日期：2006-05-01 00:00:00

abstract：:Grapes are among the widely cultivated fruit crops in the world. Grape berries like other nonclimacteric fruits undergo a complex set of dynamic, physical, physiological, and biochemical changes during ripening. Muscadine grapes are widely cultivated in the southern United States for fresh fruit and wine. To date, cha...

journal_title：Journal of proteome research

authors： Kambiranda D,Katam R,Basha SM,Siebert S

更新日期：2014-02-07 00:00:00

abstract：:Proteins and peptides present within clinical samples represent a valuable library of information regarding the ongoing processes within cells and tissues in health and disease. We have developed and validated novel technology applications that can be used to characterize the patterns of global protein expression in t...

journal_title：Journal of proteome research

authors： Marko-Varga G,Lindberg H,Löfdahl CG,Jönsson P,Hansson L,Dahlbäck M,Lindquist E,Johansson L,Foster M,Fehniger TE

更新日期：2005-07-01 00:00:00

abstract：:De Palma et al. published a research paper in which they describe the effect of chronic hypoxia on rat skeletal muscle metabolism by means of a comparative proteomic analysis (J. Proteome Res. 2007, 6(5), 1974-1984). For this, relatively young animals were used. In our communication, we note that, based on other liter...

journal_title：Journal of proteome research

authors： Gosker HR,Schols AM

更新日期：2007-09-01 00:00:00

abstract：:Since 2012, missing proteins (MPs) investigation has been one of the critical missions of Chromosome-Centric Human Proteome Project (C-HPP) through various biochemical strategies. On the basis of our previous testis MPs study, faster scanning and higher resolution mass-spectrometry-based proteomics might be conducive ...

journal_title：Journal of proteome research

authors： Wei W,Luo W,Wu F,Peng X,Zhang Y,Zhang M,Zhao Y,Su N,Qi Y,Chen L,Zhang Y,Wen B,He F,Xu P

更新日期：2016-11-04 00:00:00

abstract：:Elevated atmospheric CO2 and nitrogen are major environmental factors affecting shoot growth. The objectives of this study are to determine the interactive effects of elevated CO2 and nitrogen on leaf growth in tall fescue ( Festuca arundinacea) and to identify major proteins and associated metabolic pathways underlyi...

journal_title：Journal of proteome research

authors： Yu J,Fan N,Li R,Zhuang L,Xu Q,Huang B

更新日期：2019-06-07 00:00:00

abstract：:Interstitial cystitis/painful bladder syndrome (IC) is a chronic syndrome of unknown etiology that presents with bladder pain, urinary frequency, and urgency. The lack of specific biomarkers and a poor understanding of underlying molecular mechanisms present challenges for disease diagnosis and therapy. The goals of t...

journal_title：Journal of proteome research

authors： Wen H,Lee T,You S,Park SH,Song H,Eilber KS,Anger JT,Freeman MR,Park S,Kim J

更新日期：2015-01-02 00:00:00

abstract：:Ubiquitin ligases (E3s) serve as key regulators for the ubiquitylation-mediated pathway. The identification of the corresponding relationship between E3 and its substrates is challenging but required for understanding the regulatory network of ubiquitylation. The low abundance of ubiquitinated conjugates and high redu...

journal_title：Journal of proteome research

authors： Lan Q,Wang Y,Sun Z,Li Y,Zhang C,Chang L,Gao Y,Wu J,Wang F,Xu P

更新日期：2020-01-03 00:00:00

abstract：:Our previous study showed that concomitant use of berberine (BBR) and fluconazole (FLC) provided a synergistic action against FLC-resistant Candida albicans (C. albicans) clinical strains in vitro. To clarify the mechanism underlying this action, we performed a comparative proteomic study in untreated control cells an...

journal_title：Journal of proteome research

authors： Xu Y,Wang Y,Yan L,Liang RM,Dai BD,Tang RJ,Gao PH,Jiang YY

更新日期：2009-11-01 00:00:00

abstract：:Recent progress in genomics, proteomics, and bioinformatics enables unprecedented opportunities to examine the evolutionary history of molecular, cellular, and developmental pathways through phylogenomics. Accordingly, we have developed a motif analysis tool for phylogenomics (Phylomat, http://alg.ncsa.uiuc.edu/pmat) ...

journal_title：Journal of proteome research

authors： Graham WV,Tcheng DK,Shirk AL,Attene-Ramos MS,Welge ME,Gaskins HR

更新日期：2004-11-01 00:00:00

abstract：:Breast cancer is a leading cancer in women and is considered to be the second-most common metastatic cancer following lung cancer. An estimated 10-16% of breast cancer patients are suffering from brain metastasis, and the diagnostic cases of breast cancer brain metastasis are increasing. Nevertheless, the mechanisms b...

journal_title：Journal of proteome research

authors： Peng W,Goli M,Mirzaei P,Mechref Y

更新日期：2019-10-04 00:00:00

abstract：:Our cyanylation (CN)-based methodology for determining disulfide structure of cystinyl proteins overcomes the limitations of conventional proteolytic methods. However, the CN-based method has the potential drawback that occasionally some CN-induced cleavage fragments may not be detected. We show that CN-based methods ...

journal_title：Journal of proteome research

authors： Wu W,Huang W,Qi J,Chou YT,Torng E,Watson JT

更新日期：2004-07-01 00:00:00

abstract：:Stroke remains a major public health problem worldwide; it causes severe disability and is associated with high mortality rates. However, early diagnosis of stroke is difficult, and no reliable biomarkers are currently established. In this study, mass-spectrometry-based metabolomics was utilized to characterize the me...

journal_title：Journal of proteome research

authors： Liu P,Li R,Antonov AA,Wang L,Li W,Hua Y,Guo H,Wang L,Liu P,Chen L,Tian Y,Xu F,Zhang Z,Zhu Y,Huang Y

更新日期：2017-02-03 00:00:00

abstract：:Chinese hamster ovary (CHO) cell lines are widely used for the expression of therapeutic recombinant proteins, including monoclonal antibodies and other biologics. For manufacturing, cells derived from a single-cell clone are typically used to ensure product consistency. Presently, fetal bovine serum (FBS) is commonly...

journal_title：Journal of proteome research

authors： Lim UM,Yap MG,Lim YP,Goh LT,Ng SK

更新日期：2013-07-05 00:00:00

abstract：:Mass-spectrometry-based proteomics has become the standard approach for identifying and quantifying proteins. A vital step consists of analyzing experimentally generated mass spectra to identify the underlying peptide sequences for later mapping to the originating proteins. We here present the latest developments in S...

journal_title：Journal of proteome research

authors： Barsnes H,Vaudel M

更新日期：2018-07-06 00:00:00

abstract：:To identify proteins that are involved in the molecular mechanisms of oxygen-induced retinopathy (OIR), a well-established model of blinding ischemic retinopathy, we quantitatively analyzed the retinal proteome in a mouse model of OIR. OIR was induced by exposing C57BL/6 mice on postnatal day 7 (P7) to 75% hyperoxia f...

journal_title：Journal of proteome research

authors： Kim SJ,Jin J,Kim YJ,Kim Y,Yu HG

更新日期：2012-11-02 00:00:00

abstract：:Antibody reagents have been remained as a standard approach to characterize blood group (BG) antigens in clinic. The specificity and cross-reactivity of these BG antibodies are routine detected using the gel microcolumn assay (GMA). However, the GMA is neither specific nor sensitive, thus increasing the risk of improp...

journal_title：Journal of proteome research

authors： Yang L,Yu Y,Ma C,Wang H,Dai J,Duan H,Fu Z,Wu P,Wang D,Yu X

更新日期：2018-09-07 00:00:00

abstract：:A biphasic monolithic capillary column with 10 cm segment of strong-cation exchange monolith and 65 cm segment of reversed-phase monolith was prepared within a single 100 microm i.d. capillary. Separation performance of this column was evaluated by a five-cycle online multidimensional separation of 10 microg tryptic d...

journal_title：Journal of proteome research

authors： Wang F,Dong J,Ye M,Jiang X,Wu R,Zou H

更新日期：2008-01-01 00:00:00

abstract：:Activation of AMP-activated protein kinase (AMPK) upon energy depletion stimulates energy production and limits energy utilization. Erythrocytes lacking AMPK are susceptible to suicidal cell death (eryptosis). A hallmark of eryptosis is cell membrane scrambling with phosphatidylserine exposure at the erythrocyte surfa...

journal_title：Journal of proteome research

authors： Zelenak C,Föller M,Velic A,Krug K,Qadri SM,Viollet B,Lang F,Macek B

更新日期：2011-04-01 00:00:00

abstract：:Pancreas ductal adenocarcinoma (PDAC) remains a deadly malignancy with poor early diagnostic and no effective therapy. Although several proteomic studies have performed comparative analysis between normal and malignant tissues, there is a lack of clear characterization of proteins that could be of clinical value. Syst...

journal_title：Journal of proteome research

authors： Turtoi A,Musmeci D,Wang Y,Dumont B,Somja J,Bevilacqua G,De Pauw E,Delvenne P,Castronovo V

更新日期：2011-09-02 00:00:00

abstract：:The balance between comprehensively analyzing the proteome and using valuable mass spectrometry time is a genuine challenge in the field of proteomics. Multidimensional fractionation strategies have significantly increased proteome coverage, but often at the cost of increased mass analysis time, despite advances in ma...

journal_title：Journal of proteome research

authors： Krieger JR,Wybenga-Groot LE,Tong J,Bache N,Tsao MS,Moran MF

更新日期：2019-05-03 00:00:00

abstract：:Glioblastoma (GBM) is the most common adult primary brain tumor. Despite aggressive multimodal therapy, the survival of patients with GBM remains dismal. However, recent evidence has demonstrated the promise of bone marrow-derived mesenchymal stem cells (BM-hMSCs) as a therapeutic delivery vehicle for anti-glioma agen...

journal_title：Journal of proteome research

authors： Wildburger NC,Wood PL,Gumin J,Lichti CF,Emmett MR,Lang FF,Nilsson CL

更新日期：2015-06-05 00:00:00

abstract：:Many tumors display significant cellular heterogeneity as well as molecular heterogeneity. Sensitive biomarkers that differentiate between diagnostically challenging tumors must contend with this heterogeneity. Mass spectrometry-based molecular histology of a patient series of heterogeneous, microscopically identical ...

journal_title：Journal of proteome research

authors： Jones EA,Schmitz N,Waaijer CJ,Frese CK,van Remoortere A,van Zeijl RJ,Heck AJ,Hogendoorn PC,Deelder AM,Altelaar AF,Bovée JV,McDonnell LA

更新日期：2013-04-05 00:00:00

abstract：:Tandem mass spectrometry (MS/MS) combined with bioinformatics tools have enabled fast and systematic protein identification based on peptide-to-spectrum matches. However, it remains challenging to obtain accurate identification of endogenous peptides, such as neuropeptides, peptide hormones, peptide pheromones, venom ...

journal_title：Journal of proteome research

authors： Hayakawa E,Menschaert G,De Bock PJ,Luyten W,Gevaert K,Baggerman G,Schoofs L

更新日期：2013-12-06 00:00:00

abstract：:As cellular metabolic hubs, mitochondria are the main energy producers for the cell. These organelles host essential energy producing biochemical processes, including the TCA cycle, fatty acid oxidation, and oxidative phosphorylation. An accumulating body of literature has demonstrated that a majority of mitochondrial...

journal_title：Journal of proteome research

authors： Betsinger CN,Cristea IM

更新日期：2019-05-03 00:00:00

abstract：:Quantifying peptides based on unique peptide fragment ions avoids the issue of ratio distortion that is commonly observed for reporter ion-based quantification approaches. Herein, we present a collision-induced dissociation-cleavable, isobaric acetyl-isoleucine-proline-glycine (Ac-IPG) tag, which conserves the merits ...

journal_title：Journal of proteome research

authors： Tian X,de Vries MP,Permentier HP,Bischoff R

更新日期：2020-09-04 00:00:00

abstract：:Whole cell protein and outer membrane protein (OMP) extracts were compared for their ability to differentiate and delineate the correct database organism to an experimental sample and for the degree of dissimilarity to the nearest neighbor database organism strains. These extracts were isolated from pathogenic and non...

journal_title：Journal of proteome research

authors： Jabbour RE,Wade MM,Deshpande SV,Stanford MF,Wick CH,Zulich AW,Snyder AP

更新日期：2010-07-02 00:00:00