Phosphoproteome Response to Dithiothreitol Reveals Unique Versus Shared Features of Saccharomyces cerevisiae Stress Responses.

abstract：:In this study, a tandem LC-MS (Waters Xevo TQ) MRM-based MS method was developed for rapid, broad profiling of hydrophilic metabolites from biological samples, in either positive or negative ion modes without the need for an ion pairing reagent, using a reversed-phase pentafluorophenylpropyl (PFPP) column. The develop...

journal_title：Journal of proteome research

authors： Lv H,Palacios G,Hartil K,Kurland IJ

更新日期：2011-04-01 00:00:00

abstract：:Quantifying peptides based on unique peptide fragment ions avoids the issue of ratio distortion that is commonly observed for reporter ion-based quantification approaches. Herein, we present a collision-induced dissociation-cleavable, isobaric acetyl-isoleucine-proline-glycine (Ac-IPG) tag, which conserves the merits ...

journal_title：Journal of proteome research

authors： Tian X,de Vries MP,Permentier HP,Bischoff R

更新日期：2020-09-04 00:00:00

abstract：:Type 1 diabetes, in human patients and NOD mice, results from an immune attack on insulin-producing beta-cells of the pancreas by autoreactive T lymphocytes. In NOD mice, genetically controlled perturbations in the signaling pathways downstream of the antigen-specific T cell receptor (TCR) may be instrumental in the a...

journal_title：Journal of proteome research

authors： Iwai LK,Benoist C,Mathis D,White FM

更新日期：2010-06-04 00:00:00

abstract：:Several high-throughput statistical methods were evaluated for processing data generated by two-dimensional polyacrylamide gel electrophoresis, including how to handle missing data, normalization, and statistical analysis of data obtained from 2-D gels. Quantile normalization combined with a nonparametric permutation ...

journal_title：Journal of proteome research

authors： Chang J,Van Remmen H,Ward WF,Regnier FE,Richardson A,Cornell J

更新日期：2004-11-01 00:00:00

abstract：:Stroke remains a major public health problem worldwide; it causes severe disability and is associated with high mortality rates. However, early diagnosis of stroke is difficult, and no reliable biomarkers are currently established. In this study, mass-spectrometry-based metabolomics was utilized to characterize the me...

journal_title：Journal of proteome research

authors： Liu P,Li R,Antonov AA,Wang L,Li W,Hua Y,Guo H,Wang L,Liu P,Chen L,Tian Y,Xu F,Zhang Z,Zhu Y,Huang Y

更新日期：2017-02-03 00:00:00

abstract：:Hematopoietic stem cell transplantation is the oldest and most successful form of stem cell therapy. High dose therapy (HDT) followed by hematopoietic stem cell transplantation allows physicians to administer increased amounts of chemotherapy and/or radiation while minimizing negative side effects such as damage to bl...

journal_title：Journal of proteome research

authors： Cano KE,Li L,Bhatia S,Bhatia R,Forman SJ,Chen Y

更新日期：2011-06-03 00:00:00

abstract：:In this paper, we describe a method for analyzing large-volume protein samples using capillary electrophoresis in conjunction with laser-induced fluorescence detection (CE-LIF). To improve the stacking and separation efficiencies of proteins, we added either 0.01% sodium dodecyl sulfate (SDS) or 0.01% poly(ethylene ox...

journal_title：Journal of proteome research

authors： Huang YF,Hsieh MM,Tseng WL,Chang HT

更新日期：2006-02-01 00:00:00

abstract：:To further our understanding of the biology of the thermophilic bacterium Geobacillus thermoleovorans T80, we now report the first proteomic analysis of the insoluble subproteome of this isolate. A combination of both shotgun and multidimensional methodologies were utilized, and a total of 8628 peptides was initially ...

journal_title：Journal of proteome research

authors： Graham RL,O'Loughlin SN,Pollock CE,Ternan NG,Weatherly DB,Jackson PJ,Tarleton RL,McMullan G

更新日期：2006-09-01 00:00:00

abstract：:The nation-wide electronic health record database acts as an interoperable repository of health data obtained throughout citizen contacts with health care providers. This system improves accessibility for citizens and researchers to health data with the ability to assign context to disease development. In that system,...

journal_title：Journal of proteome research

authors： Sepper R,Ross P,Tiik M

更新日期：2011-01-07 00:00:00

abstract：:The aim of current study was to investigate the metabolic changes associated with histidine supplementation in serum and urine metabolic signatures and serum amino acid (AA) profiles. Serum and urine 1H NMR-based metabolomics and serum AA profiles were employed in 32 and 37 obese women with metabolic syndrome (MetS) i...

journal_title：Journal of proteome research

authors： Du S,Sun S,Liu L,Zhang Q,Guo F,Li C,Feng R,Sun C

更新日期：2017-06-02 00:00:00

abstract：:POPX2 is a serine/threonine phosphatase belonging to the protein phosphatase 2C (PP2C) family that has been found to be elevated in invasive breast cancer cells. Silencing of POPX2 results in lower cell motility and invasiveness. The molecular mechanism of POPX2-regulated cell motility is not well understood. To ident...

journal_title：Journal of proteome research

authors： Zhang S,Guo T,Chan H,Sze SK,Koh CG

更新日期：2013-06-07 00:00:00

abstract：:To estimate the potential of the state-of-the-art proteomics technologies on full coverage of the encoding gene products, the Chinese Human Chromosome Proteome Consortium (CCPC) applied a multiomics strategy to systematically analyze the transciptome, translatome, and proteome of the same cultured hepatoma cells with ...

journal_title：Journal of proteome research

authors： Chang C,Li L,Zhang C,Wu S,Guo K,Zi J,Chen Z,Jiang J,Ma J,Yu Q,Fan F,Qin P,Han M,Su N,Chen T,Wang K,Zhai L,Zhang T,Ying W,Xu Z,Zhang Y,Liu Y,Liu X,Zhong F,Shen H,Wang Q,Hou G,Zhao H,Li G,Liu S,G

更新日期：2014-01-03 00:00:00

abstract：:Cyadox is an antibiotic drug and has the potential to be used as a feedstuff additive in promoting the growth of animals. However, the toxicity of cyadox should be fully assessed before application, and this has prompted the current investigation on the metabolic responses of mice to cyadox exposure, using a metabonom...

journal_title：Journal of proteome research

authors： Huang C,Lei H,Zhao X,Tang H,Wang Y

更新日期：2013-01-04 00:00:00

abstract：:Peptide amidination labeling using S-methyl thioacetimidate (SMTA) is investigated in an attempt to increase the number and types of peptides that can be detected in a bottom-up proteomics experiment. This derivatization method affects the basicity of lysine residues and is shown here to significantly impact the idios...

journal_title：Journal of proteome research

authors： Li S,Dabir A,Misal SA,Tang H,Radivojac P,Reilly JP

更新日期：2016-10-07 00:00:00

abstract：:The present study was undertaken to identify proteins interacting with PrP(C) that could provide new insights into its physiological functions and pathological role. Human PrP(C) was expressed in prion protein-deficient murine hippocampus (HpL3-4) neuronal cells. The PrP(C) along with its interacting proteins were aff...

journal_title：Journal of proteome research

authors： Zafar S,von Ahsen N,Oellerich M,Zerr I,Schulz-Schaeffer WJ,Armstrong VW,Asif AR

更新日期：2011-07-01 00:00:00

abstract：:Our previous study showed that concomitant use of berberine (BBR) and fluconazole (FLC) provided a synergistic action against FLC-resistant Candida albicans (C. albicans) clinical strains in vitro. To clarify the mechanism underlying this action, we performed a comparative proteomic study in untreated control cells an...

journal_title：Journal of proteome research

authors： Xu Y,Wang Y,Yan L,Liang RM,Dai BD,Tang RJ,Gao PH,Jiang YY

更新日期：2009-11-01 00:00:00

abstract：:Spontaneous equine recurrent uveitis (ERU) is an incurable autoimmune disease affecting the eye. Although retinal-autoantigen specific T-helper 1 cells have been demonstrated to trigger disease progression and relapses, the molecular processes leading to retinal degeneration and consequent blindness remain unknown. To...

journal_title：Journal of proteome research

authors： Hauck SM,Schoeffmann S,Amann B,Stangassinger M,Gerhards H,Ueffing M,Deeg CA

更新日期：2007-06-01 00:00:00

abstract：:Middle-aged and old left ventricles (LVs) are structurally and functionally very similar. Compared to a young LV, both show increased wall thickness and increased cavity size, with preserved cardiac function. However, when a stressor such as myocardial infarction occurs, striking differences are revealed between young...

journal_title：Journal of proteome research

authors： Dai Q,Escobar GP,Hakala KW,Lambert JM,Weintraub ST,Lindsey ML

更新日期：2008-02-01 00:00:00

abstract：:Protein lysine acylation, including acetylation and succinylation, has been found to be a major post-translational modification (PTM) and is associated with the regulation of cellular processes that are widespread in bacteria. Vibrio parahemolyticus is a model marine bacterium that causes seafood-borne illness in huma...

journal_title：Journal of proteome research

authors： Pan J,Chen R,Li C,Li W,Ye Z

更新日期：2015-10-02 00:00:00

abstract：:Improvements in proteome analysis of erythrocyte membrane proteins by two-dimensional electrophoresis are here reported. In particular, a differential extraction procedure was set up allowing separation of integral membrane proteins from peripheral species. Moreover, the use of dilute Immobiline gels (down to as low a...

journal_title：Journal of proteome research

authors： Bruschi M,Seppi C,Arena S,Musante L,Santucci L,Balduini C,Scaloni A,Lanciotti M,Righetti PG,Candiano G

更新日期：2005-07-01 00:00:00

abstract：:Protein phosphorylation is a globally adopted and tightly controlled post-translational modification, and represents one of the most important molecular switching mechanisms that govern the entire spectrum of biological processes. In the central nervous system, it has been demonstrated that phosphorylation of key prot...

journal_title：Journal of proteome research

authors： Liao L,McClatchy DB,Park SK,Xu T,Lu B,Yates JR 3rd

更新日期：2008-11-01 00:00:00

abstract：:The plant cell wall is a dynamic cellular compartment consisting of a complex matrix of components that can change dramatically in response to environmental stresses. During pathogen attack, for instance, a wide spectrum of proteins that participate in various sequential processes involved in plant defense is secreted...

journal_title：Journal of proteome research

authors： Cheng FY,Blackburn K,Lin YM,Goshe MB,Williamson JD

更新日期：2009-01-01 00:00:00

abstract：:Moving from macroscale preparative systems in proteomics to micro- and nanotechnologies offers researchers the ability to deeply profile smaller numbers of cells that are more likely to be encountered in clinical settings. Herein a recently developed microscale proteomic method, microdroplet processing in one pot for ...

journal_title：Journal of proteome research

authors： Weke K,Singh A,Uwugiaren N,Alfaro JA,Wang T,Hupp TR,O'Neill JR,Vojtesek B,Goodlett DR,Williams SM,Zhou M,Kelly RT,Zhu Y,Dapic I

更新日期：2021-01-25 00:00:00

abstract：:The low selectivity of Kv1 peptide inhibitors for specific isoforms makes them poor candidates for the development of theraputics. Using combined approaches, we showed that the Kv1 turret is the critical determinant for ADWX-1 peptide inhibitor selectivity of Kv1.3 over Kv1.1. Mutation of Kv1.1 turret residues to matc...

journal_title：Journal of proteome research

authors： Yin SJ,Jiang L,Yi H,Han S,Yang DW,Liu ML,Liu H,Cao ZJ,Wu YL,Li WX

更新日期：2008-11-01 00:00:00

abstract：:An alternative sieving matrix, replaceable cross-linked polyacrylamide (rCPA), was developed for sodium dodecyl sulfate capillary gel electrophoresis (SDS-CGE) separation of proteins. This rCPA could be conveniently pressurized into separation capillaries under a pressure of 80 psi. SDS-CGE separations using this matr...

journal_title：Journal of proteome research

authors： Lu JJ,Liu S,Pu Q

更新日期：2005-05-01 00:00:00

abstract：:The paper describes the implementation of a software system based on the Fenyö disulfide bond assignment algorithm. The system allows an investigator to enter data derived from mass spectrum peak assignments, a target protein sequence and other experimental conditions. The output of the system is the set of disulfide ...

journal_title：Journal of proteome research

authors： Craig R,Krokhin O,Wilkins J,Beavis RC

更新日期：2003-11-01 00:00:00

abstract：:Many pressing medical challenges, such as diagnosing disease, enhancing directed stem-cell differentiation, and classifying cancers, have long been hindered by limitations in our ability to quantify proteins in single cells. Mass spectrometry (MS) is poised to transcend these limitations by developing powerful methods...

journal_title：Journal of proteome research

authors： Specht H,Slavov N

更新日期：2018-08-03 00:00:00

abstract：:MS-based de novo peptide sequencing has been improved remarkably with significant development of mass-spectrometry and computational approaches but still lacks quality-control methods. Here we proposed a novel algorithm pSite to evaluate the confidence of each amino acid rather than the full-length peptides obtained b...

journal_title：Journal of proteome research

authors： Yang H,Chi H,Zhou WJ,Zeng WF,Liu C,Wang RM,Wang ZW,Niu XN,Chen ZL,He SM

更新日期：2018-01-05 00:00:00

abstract：:Chronic Obstructive Pulmonary Disease (COPD), a lung disease related to smoking, is one of the leading causes of chronic morbidity and mortality around the world. One goal in COPD research is the identification of biomarkers for early diagnosis of the disease. Here, we sought COPD-specific changes in the proteome from...

journal_title：Journal of proteome research

authors： Ohlmeier S,Vuolanto M,Toljamo T,Vuopala K,Salmenkivi K,Myllärniemi M,Kinnula VL

更新日期：2008-12-01 00:00:00

abstract：:Huntington's disease (HD) causes widespread CNS changes and systemic abnormalities including endocrine and immune dysfunction. HD biomarkers are needed to power clinical trials of potential treatments. We used multiplatform proteomic profiling to reveal plasma changes with HD progression. Proteins of interest were eva...

journal_title：Journal of proteome research

authors： Dalrymple A,Wild EJ,Joubert R,Sathasivam K,Björkqvist M,Petersén A,Jackson GS,Isaacs JD,Kristiansen M,Bates GP,Leavitt BR,Keir G,Ward M,Tabrizi SJ

更新日期：2007-07-01 00:00:00