Replaceable cross-linked polyacrylamide for high performance separation of proteins.

abstract：:Many proteins possess intrinsic disorder (ID) and lack a rigid three-dimensional structure in at least part of their sequence. ID has been hypothesized to influence protein-protein and protein-ligand interactions. We calculated ID for nearly 400 vertebrate and invertebrate members of the biomedically important nuclear...

journal_title：Journal of proteome research

authors： Krasowski MD,Reschly EJ,Ekins S

更新日期：2008-10-01 00:00:00

abstract：:Oligomeric amyloid-β is currently of interest in amyloid-β mediated toxicity and the pathogenesis of Alzheimer's disease. Mapping the amyloid-β interaction partners could help to discover novel pathways in disease pathogenesis. To discover the amyloid-β interaction partners, we applied a protein array with more than 8...

journal_title：Journal of proteome research

authors： Virok DP,Simon D,Bozsó Z,Rajkó R,Datki Z,Bálint É,Szegedi V,Janáky T,Penke B,Fülöp L

更新日期：2011-04-01 00:00:00

abstract：:Mass spectrometry (MS) has become an accessible tool for whole proteome quantitation with the ability to characterize protein expression across thousands of proteins within a single experiment. A subset of MS quantification methods (e.g., SILAC and label-free) monitor the relative intensity of intact peptides, where t...

journal_title：Journal of proteome research

authors： O'Brien JJ,O'Connell JD,Paulo JA,Thakurta S,Rose CM,Weekes MP,Huttlin EL,Gygi SP

更新日期：2018-01-05 00:00:00

abstract：:A database of nonredundant structures of EF-hand domains--i.e., pairs of helix-loop-helix motifs--has been assembled, and the six angles among the four helices re-determined. A principal component analysis of these angles allows us to use two such components (PC1 and PC2) to describe the system retaining 80% of the to...

journal_title：Journal of proteome research

authors： Babini E,Bertini I,Capozzi F,Luchinat C,Quattrone A,Turano M

更新日期：2005-11-01 00:00:00

abstract：:Clostridium difficile in recent years has undergone rapid evolution and has emerged as a serious human pathogen. Proteomic approaches can improve the understanding of the diversity of this important pathogen, especially in comparing the adaptive ability of different C. difficile strains. In this study, TMT labeling an...

journal_title：Journal of proteome research

authors： Chen JW,Scaria J,Mao C,Sobral B,Zhang S,Lawley T,Chang YF

更新日期：2013-03-01 00:00:00

abstract：:Francisella tularensis causes disease (tularemia) in a large number of mammals, including man. We previously demonstrated enhanced efficacy of conventional antibiotic therapy for tularemia by postexposure passive transfer of immune sera developed against a F. tularensis LVS membrane protein fraction (MPF). However, th...

journal_title：Journal of proteome research

authors： Chandler JC,Sutherland MD,Harton MR,Molins CR,Anderson RV,Heaslip DG,Bosio CM,Belisle JT

更新日期：2015-02-06 00:00:00

abstract：:Oxidative stress and inflammation play a role in the physiopathology of insulin resistance, diabetes and cardiovascular disease. A single high-fat, high-carbohydrate (HFHC) meal induces an increase in inflammatory and oxidative stress markers in peripheral blood mononuclear cells (PBMC). Previous studies have shown th...

journal_title：Journal of proteome research

authors： Chaves DFS,Carvalho PC,Brasili E,Rogero MM,Hassimotto NA,Diedrich JK,Moresco JJ,Yates JR 3rd,Lajolo FM

更新日期：2017-11-03 00:00:00

abstract：:A comprehensive study of the molecular active landscape of human cells can be undertaken to integrate two different but complementary perspectives: transcriptomics, and proteomics. After the genome era, proteomics has emerged as a powerful tool to simultaneously identify and characterize the compendium of thousands of...

journal_title：Journal of proteome research

authors： Díez P,Droste C,Dégano RM,González-Muñoz M,Ibarrola N,Pérez-Andrés M,Garin-Muga A,Segura V,Marko-Varga G,LaBaer J,Orfao A,Corrales FJ,De Las Rivas J,Fuentes M

更新日期：2015-09-04 00:00:00

abstract：:New mass-tagging reagents for quantitative proteomics measurements have been designed using solid phase peptide synthesis technology. The solid phase mass tags have been used to accurately measure the relative amounts of cysteine-containing peptides in model peptide mixtures as well as in mixtures of tryptic digests i...

journal_title：Journal of proteome research

authors： Shi Y,Xiang R,Crawford JK,Colangelo CM,Horváth C,Wilkins JA

更新日期：2004-01-01 00:00:00

abstract：:The chromatophore membrane of the photosynthetic diazotroph Rhodospirillum rubrum is of vital importance for a number of central processes, including nitrogen fixation. Using a novel amphiphile, we have identified protein complexes present under different nitrogen availability conditions by the use of two-dimensional ...

journal_title：Journal of proteome research

authors： Selao TT,Branca R,Chae PS,Lehtiö J,Gellman SH,Rasmussen SG,Nordlund S,Norén A

更新日期：2011-06-03 00:00:00

abstract：:To further our understanding of the biology of the thermophilic bacterium Geobacillus thermoleovorans T80, we now report the first proteomic analysis of the insoluble subproteome of this isolate. A combination of both shotgun and multidimensional methodologies were utilized, and a total of 8628 peptides was initially ...

journal_title：Journal of proteome research

authors： Graham RL,O'Loughlin SN,Pollock CE,Ternan NG,Weatherly DB,Jackson PJ,Tarleton RL,McMullan G

更新日期：2006-09-01 00:00:00

abstract：:High-affinity antibodies binding to linear peptides in solution are a prerequisite for performing immuno-MRM, an emerging technology for protein quantitation with high precision and specificity using peptide immunoaffinity enrichment coupled to stable isotope dilution and targeted mass spectrometry. Recombinant antibo...

journal_title：Journal of proteome research

authors： Whiteaker JR,Zhao L,Frisch C,Ylera F,Harth S,Knappik A,Paulovich AG

更新日期：2014-04-04 00:00:00

abstract：:The interaction between the immune system and invading bacteria is sufficient to eradicate microorganisms for the majority of bacterial infections, but suppression of the microbicidal response leads to reactivation or chronic evolution of infections and to bacterial persistence. To identify the cellular pathways affec...

journal_title：Journal of proteome research

authors： Vranakis I,De Bock PJ,Papadioti A,Samoilis G,Tselentis Y,Gevaert K,Tsiotis G,Psaroulaki A

更新日期：2011-09-02 00:00:00

abstract：:Populations around the world are aging rapidly. Age-related loss of physiological functions negatively affects quality of life. A major contributor to the frailty syndrome of aging is loss of skeletal muscle. In this study we assessed the skeletal muscle biopsy metabolome of healthy young, healthy older and frail olde...

journal_title：Journal of proteome research

authors： Fazelzadeh P,Hangelbroek RW,Tieland M,de Groot LC,Verdijk LB,van Loon LJ,Smilde AK,Alves RD,Vervoort J,Müller M,van Duynhoven JP,Boekschoten MV

更新日期：2016-02-05 00:00:00

abstract：:Renal cell carcinoma (RCC) tissue is composed of a mixture of neoplastic and normal cells, which complicate proteome analysis. The aim of our study was to investigate whether it is feasible to establish primary cell cultures of RCC and of renal cortex maintaining the tissue phenotype along with a more homogeneous and ...

journal_title：Journal of proteome research

authors： Perego RA,Bianchi C,Corizzato M,Eroini B,Torsello B,Valsecchi C,Di Fonzo A,Cordani N,Favini P,Ferrero S,Pitto M,Sarto C,Magni F,Rocco F,Mocarelli P

更新日期：2005-09-01 00:00:00

abstract：:Imatinib is the first molecular targeted therapy that has shown clinical success, but imatinib acquired resistance, although a rare event, is critical during the therapy of chronic myelogenous leukaemia (CML). With the aim of better understanding the molecular mechanisms accompanying acquisition of resistance to this ...

journal_title：Journal of proteome research

authors： Ferrari G,Pastorelli R,Buchi F,Spinelli E,Gozzini A,Bosi A,Santini V

更新日期：2007-01-01 00:00:00

abstract：:Birch pollen allergy is diagnosed and treated with aqueous extracts from birch pollen, which contain a mixture of allergens and nonallergenic proteins, including large numbers of closely related sequence variants, so-called iso-allergens of the major allergen, Bet v 1. The quality of therapeutic and diagnostic allerge...

journal_title：Journal of proteome research

authors： Spiric J,Schulenborg T,Schwaben L,Engin AM,Karas M,Reuter A

更新日期：2017-10-06 00:00:00

abstract：:Salt stress is a major abiotic factor limiting maize yield. To characterize the mechanism underlying maize salt tolerance, we compared the seedling root proteomes of salt-tolerant Jing724 and salt-sensitive D9H. The germination rate and growth parameter values (weight and length) were higher for Jing724 than for D9H u...

journal_title：Journal of proteome research

authors： Luo M,Zhao Y,Wang Y,Shi Z,Zhang P,Zhang Y,Song W,Zhao J

更新日期：2018-01-05 00:00:00

abstract：:Antibodies are a primary research tool for a diverse range of experiments in biology, from development to pathology. Their utility is derived from their ability to specifically identify proteins at a high level of sensitivity. This diversity of experimental requirements stretches the capabilities of these key research...

journal_title：Journal of proteome research

authors： Haslam NJ,Gibson TJ

更新日期：2010-07-02 00:00:00

abstract：:O-Linked N-acetyl-β-d-glucosamine (O-GlcNAc) is a dynamic post-translational modification that modifies and regulates over 3000 nuclear, cytoplasmic, and mitochondrial proteins. Upon exposure to stress and injury, cells and tissues increase the O-GlcNAc modification, or O-GlcNAcylation, of numerous proteins promoting ...

journal_title：Journal of proteome research

authors： Lee A,Miller D,Henry R,Paruchuri VD,O'Meally RN,Boronina T,Cole RN,Zachara NE

更新日期：2016-12-02 00:00:00

abstract：:Genomic-scale analysis of protein function is currently limited by the ability to rapidly express the enormous diversity of protein targets in their active form. We describe a method to construct transcriptionally active expression templates (ETs) in parallel using a single PCR step wherein the overlap-extension react...

journal_title：Journal of proteome research

authors： Woodrow KA,Airen IO,Swartz JR

更新日期：2006-12-01 00:00:00

abstract：:Protein biomarker discovery in the low concentration range of human body fluids requires the enrichment of the proteins of interest. Here we report on a tandem affinity strategy: In the first step, we isolated a human plasma glyco-subproteome of healthy individuals by wheat germ agglutinin (WGA) lectin affinity chroma...

journal_title：Journal of proteome research

authors： Mortezai N,Harder S,Schnabel C,Moors E,Gauly M,Schlüter H,Wagener C,Buck F

更新日期：2010-12-03 00:00:00

abstract：:A fast and robust method for lipid profiling utilizing liquid chromatography coupled with mass spectrometry has been demonstrated and validated for the analysis of human plasma. This method allowed quantification and identification of lipids in human plasma using parallel alternating low energy and high energy collisi...

journal_title：Journal of proteome research

authors： Castro-Perez JM,Kamphorst J,DeGroot J,Lafeber F,Goshawk J,Yu K,Shockcor JP,Vreeken RJ,Hankemeier T

更新日期：2010-05-07 00:00:00

abstract：:Oxidative injury is believed to play an important role in the pathogenesis of lung diseases such as emphysema and lung cancer. We examined the effects of a classic reactive oxygen species, H 2O 2, on the hydrogen peroxide response proteins (HPRP) in human pneumocytes using comparative two-dimensional gel electrophores...

journal_title：Journal of proteome research

authors： Duan X,Kelsen SG,Merali S

更新日期：2008-11-01 00:00:00

abstract：:Plant secretory (Class III) peroxidases are redox enzymes that rely on N-glycosylation for full enzyme activity and stability. Peroxidases from palm tree leaves comprise the most stable and active plant peroxidases characterized to date. Herein, site-specific glycosylation and microheterogeneity of windmill palm tree ...

journal_title：Journal of proteome research

authors： Baker MR,Tabb DL,Ching T,Zimmerman LJ,Sakharov IY,Li QX

更新日期：2016-06-03 00:00:00

abstract：:Reversible phosphorylations play a critical role in most biological pathways. Hence, in signaling studies great effort has been put into identification of a maximum number of phosphosites per experiment. Mass spectrometry (MS)-based phosphoproteomics approaches have been proven to be an ideal analytical method for map...

journal_title：Journal of proteome research

authors： Zarei M,Sprenger A,Metzger F,Gretzmeier C,Dengjel J

更新日期：2011-08-05 00:00:00

abstract：:Understanding how bacteria survive periods of cell wall stress is of fundamental interest and can help generate ideas for improved antibacterial treatments. In this study we use tandem mass tagging to characterize the proteomic response of vancomycin resistant Streptomyces coelicolor to the exposure to sublethal level...

journal_title：Journal of proteome research

authors： Hesketh A,Deery MJ,Hong HJ

更新日期：2015-07-02 00:00:00

abstract：:Diosgenin (DIO), the starting material for the synthesis of steroidal anti-inflammatory drugs in the pharmaceutical industry, has been previously demonstrated to display pharmaceutical effects against cerebral ischemic reperfusion (I/R). However, the alterations of brain proteome profiles underlying this treatment rem...

journal_title：Journal of proteome research

authors： Zhang X,Wang X,Khurm M,Zhan G,Zhang H,Ito Y,Guo Z

更新日期：2020-03-06 00:00:00

abstract：:Facing the explosion of newly generated protein sequences in the post genomic era, we are challenged to develop an automated method for fast and reliably annotating their subcellular locations. Knowledge of subcellular locations of proteins can provide useful hints for revealing their functions and understanding how t...

journal_title：Journal of proteome research

authors： Chou KC,Shen HB

更新日期：2006-08-01 00:00:00

abstract：:An efficient means for the identification of prognostic and predictive biomarkers is essential in today's cancer management. A new approach toward biomarker discovery has therefore been proposed, where pathways instead of individual proteins would be monitored and targeted. Recently, the 'secretome', a biological flui...

journal_title：Journal of proteome research

authors： Lawlor K,Nazarian A,Lacomis L,Tempst P,Villanueva J

更新日期：2009-03-01 00:00:00